Strain Fitness in Escherichia coli BW25113 around b1960

Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyedA and vsr overlap by 12 nucleotidesvsr and dcm overlap by 20 nucleotides b1959: yedA - predicted inner membrane protein (NCBI), at 2,027,563 to 2,028,483 yedA b1960: vsr - DNA mismatch endonuclease of very short patch repair (NCBI), at 2,028,472 to 2,028,942 vsr b1961: dcm - DNA cytosine methylase (NCBI), at 2,028,923 to 2,030,341 dcm Position (kb) 2028 2029Strain fitness (log2 ratio) -1 0 1at 2027.608 kb on + strandat 2027.686 kb on + strand, within yedAat 2027.723 kb on + strand, within yedAat 2028.038 kb on + strand, within yedAat 2028.081 kb on - strand, within yedAat 2028.322 kb on + strand, within yedAat 2028.398 kb on + strandat 2028.717 kb on + strand, within vsrat 2028.727 kb on - strand, within vsrat 2028.727 kb on - strand, within vsrat 2028.753 kb on + strand, within vsrat 2028.753 kb on + strand, within vsrat 2028.819 kb on - strand, within vsrat 2028.819 kb on - strand, within vsrat 2028.943 kb on + strandat 2028.966 kb on + strandat 2028.966 kb on - strandat 2029.342 kb on + strandat 2029.361 kb on - strand, within dcm

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
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2,027,608 + +0.3
2,027,686 + yedA b1959 0.13 +0.1
2,027,723 + yedA b1959 0.17 -0.3
2,028,038 + yedA b1959 0.52 +0.4
2,028,081 - yedA b1959 0.56 -0.3
2,028,322 + yedA b1959 0.82 +0.7
2,028,398 + +0.5
2,028,717 + vsr b1960 0.52 +0.2
2,028,727 - vsr b1960 0.54 +0.7
2,028,727 - vsr b1960 0.54 +0.3
2,028,753 + vsr b1960 0.60 +0.8
2,028,753 + vsr b1960 0.60 -0.2
2,028,819 - vsr b1960 0.74 +1.0
2,028,819 - vsr b1960 0.74 +0.3
2,028,943 + +0.7
2,028,966 + +0.0
2,028,966 - +0.2
2,029,342 + -0.3
2,029,361 - dcm b1961 0.31 +1.1

Or see this region's nucleotide sequence