Strain Fitness in Escherichia coli BW25113 around b1103

Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfhuE and ycfF are separated by 333 nucleotidesycfF and ycfL are separated by 2 nucleotidesycfL and ycfM are separated by 13 nucleotides b1102: fhuE - ferric-rhodotorulic acid outer membrane transporter (NCBI), at 1,158,585 to 1,160,774 fhuE b1103: ycfF - orf, hypothetical protein (VIMSS), at 1,161,108 to 1,161,467 ycfF b1104: ycfL - hypothetical protein (NCBI), at 1,161,470 to 1,161,847 ycfL b1105: ycfM - predicted outer membrane lipoprotein (NCBI), at 1,161,861 to 1,162,502 ycfM Position (kb) 1161 1162Strain fitness (log2 ratio) -3 -2 -1 0 1at 1160.304 kb on - strand, within fhuEat 1160.304 kb on - strand, within fhuEat 1160.420 kb on - strand, within fhuEat 1160.735 kb on - strandat 1160.799 kb on + strandat 1161.023 kb on + strandat 1161.023 kb on + strandat 1161.034 kb on + strandat 1161.048 kb on - strandat 1161.048 kb on - strandat 1161.180 kb on - strand, within ycfFat 1161.414 kb on - strand, within ycfFat 1161.508 kb on - strand, within ycfLat 1161.648 kb on - strand, within ycfLat 1161.659 kb on + strand, within ycfLat 1161.996 kb on + strand, within ycfMat 1162.014 kb on + strand, within ycfMat 1162.014 kb on + strand, within ycfMat 1162.190 kb on - strand, within ycfMat 1162.347 kb on - strand, within ycfMat 1162.456 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
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1,160,304 - fhuE b1102 0.78 -0.1
1,160,304 - fhuE b1102 0.78 -0.6
1,160,420 - fhuE b1102 0.84 +0.2
1,160,735 - -0.4
1,160,799 + +0.3
1,161,023 + -3.4
1,161,023 + -0.2
1,161,034 + +0.2
1,161,048 - -0.4
1,161,048 - -1.4
1,161,180 - ycfF b1103 0.20 +0.8
1,161,414 - ycfF b1103 0.85 +0.3
1,161,508 - ycfL b1104 0.10 -0.3
1,161,648 - ycfL b1104 0.47 +0.7
1,161,659 + ycfL b1104 0.50 -0.1
1,161,996 + ycfM b1105 0.21 -0.4
1,162,014 + ycfM b1105 0.24 -0.9
1,162,014 + ycfM b1105 0.24 -0.2
1,162,190 - ycfM b1105 0.51 +0.3
1,162,347 - ycfM b1105 0.76 +0.2
1,162,456 - -0.2

Or see this region's nucleotide sequence