Strain Fitness in Escherichia coli BW25113 around b0698

Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntkdpB and kdpA are separated by 22 nucleotideskdpA and kdpF overlap by 1 nucleotideskdpF and ybfA are separated by 312 nucleotidesybfA and rhsC are separated by 242 nucleotides b0697: kdpB - potassium-transporting ATPase subunit B (NCBI), at 724,211 to 726,259 kdpB b0698: kdpA - potassium-transporting ATPase subunit A (NCBI), at 726,282 to 727,955 kdpA b4513: kdpF - potassium ion accessory transporter subunit (NCBI), at 727,955 to 728,044 kdpF b0699: ybfA - hypothetical protein (NCBI), at 728,357 to 728,563 ybfA b0700: rhsC - rhsC element core protein RshC (NCBI), at 728,806 to 732,999 rhsC Position (kb) 726 727 728Strain fitness (log2 ratio) -2 -1 0 1at 725.492 kb on + strand, within kdpBat 725.520 kb on - strand, within kdpBat 725.553 kb on - strand, within kdpBat 725.553 kb on - strand, within kdpBat 725.667 kb on + strand, within kdpBat 725.705 kb on + strand, within kdpBat 725.778 kb on - strand, within kdpBat 725.839 kb on + strand, within kdpBat 725.871 kb on - strand, within kdpBat 725.966 kb on + strand, within kdpBat 725.974 kb on - strand, within kdpBat 726.039 kb on - strand, within kdpBat 726.117 kb on - strandat 726.247 kb on - strandat 726.247 kb on - strandat 726.263 kb on - strandat 726.331 kb on - strandat 726.331 kb on - strandat 726.353 kb on - strandat 726.388 kb on + strandat 726.435 kb on - strandat 726.561 kb on - strand, within kdpAat 726.580 kb on + strand, within kdpAat 726.590 kb on - strand, within kdpAat 726.590 kb on - strand, within kdpAat 726.752 kb on + strand, within kdpAat 726.945 kb on - strand, within kdpAat 726.981 kb on + strand, within kdpAat 727.062 kb on - strand, within kdpAat 727.075 kb on - strand, within kdpAat 727.075 kb on - strand, within kdpAat 727.107 kb on - strand, within kdpAat 727.325 kb on - strand, within kdpAat 727.338 kb on + strand, within kdpAat 727.338 kb on + strand, within kdpAat 727.435 kb on - strand, within kdpAat 727.464 kb on + strand, within kdpAat 727.600 kb on + strand, within kdpAat 727.600 kb on + strand, within kdpAat 727.640 kb on - strand, within kdpAat 727.687 kb on - strand, within kdpAat 727.692 kb on - strand, within kdpAat 727.696 kb on + strand, within kdpAat 727.696 kb on + strand, within kdpAat 727.696 kb on + strand, within kdpAat 727.767 kb on + strand, within kdpAat 727.792 kb on + strandat 727.871 kb on + strandat 727.871 kb on + strandat 727.884 kb on + strandat 727.904 kb on - strandat 727.904 kb on - strandat 727.963 kb on + strandat 727.966 kb on - strandat 727.988 kb on - strand, within kdpFat 728.022 kb on - strand, within kdpFat 728.054 kb on - strandat 728.054 kb on - strandat 728.059 kb on - strandat 728.068 kb on - strandat 728.076 kb on - strandat 728.580 kb on + strandat 728.597 kb on + strandat 728.641 kb on + strandat 728.641 kb on + strandat 728.758 kb on - strandat 728.810 kb on - strandat 728.840 kb on - strandat 728.863 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
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725,492 + kdpB b0697 0.63 -1.3
725,520 - kdpB b0697 0.64 -0.8
725,553 - kdpB b0697 0.65 -0.5
725,553 - kdpB b0697 0.65 -0.6
725,667 + kdpB b0697 0.71 -1.2
725,705 + kdpB b0697 0.73 -0.2
725,778 - kdpB b0697 0.76 -0.2
725,839 + kdpB b0697 0.79 -0.4
725,871 - kdpB b0697 0.81 -0.6
725,966 + kdpB b0697 0.86 -0.8
725,974 - kdpB b0697 0.86 -1.0
726,039 - kdpB b0697 0.89 -0.4
726,117 - -0.2
726,247 - -1.0
726,247 - +0.7
726,263 - -1.3
726,331 - -0.0
726,331 - -0.4
726,353 - -0.5
726,388 + -0.6
726,435 - -0.8
726,561 - kdpA b0698 0.17 -0.4
726,580 + kdpA b0698 0.18 -0.5
726,590 - kdpA b0698 0.18 -0.6
726,590 - kdpA b0698 0.18 -1.1
726,752 + kdpA b0698 0.28 +1.1
726,945 - kdpA b0698 0.40 -1.0
726,981 + kdpA b0698 0.42 -0.6
727,062 - kdpA b0698 0.47 -0.1
727,075 - kdpA b0698 0.47 -1.1
727,075 - kdpA b0698 0.47 -0.9
727,107 - kdpA b0698 0.49 -1.7
727,325 - kdpA b0698 0.62 -0.5
727,338 + kdpA b0698 0.63 -0.7
727,338 + kdpA b0698 0.63 -0.9
727,435 - kdpA b0698 0.69 -0.6
727,464 + kdpA b0698 0.71 -1.0
727,600 + kdpA b0698 0.79 -0.0
727,600 + kdpA b0698 0.79 -1.1
727,640 - kdpA b0698 0.81 -0.6
727,687 - kdpA b0698 0.84 -0.6
727,692 - kdpA b0698 0.84 -0.7
727,696 + kdpA b0698 0.84 -0.5
727,696 + kdpA b0698 0.84 +1.2
727,696 + kdpA b0698 0.84 -0.3
727,767 + kdpA b0698 0.89 -1.1
727,792 + -0.9
727,871 + -0.6
727,871 + -0.7
727,884 + -1.9
727,904 - -0.6
727,904 - -0.4
727,963 + -1.0
727,966 - +0.1
727,988 - kdpF b4513 0.37 -0.4
728,022 - kdpF b4513 0.74 -1.2
728,054 - -0.7
728,054 - -0.4
728,059 - -0.1
728,068 - +0.8
728,076 - +1.0
728,580 + +0.5
728,597 + +0.1
728,641 + +0.3
728,641 + +0.0
728,758 - +0.4
728,810 - +0.3
728,840 - +0.2
728,863 - +0.1

Or see this region's nucleotide sequence