Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ylcH and ybcN overlap by 4 nucleotides ybcN and ninE overlap by 1 nucleotides ninE and ybcO overlap by 8 nucleotides ybcO and rusA overlap by 4 nucleotides rusA and ylcG overlap by 4 nucleotides ylcG and ybcQ are separated by 85 nucleotides ybcQ and nmpC are separated by 189 nucleotides
b4588: ylcH - hypothetical protein, DLP12 prophage (RefSeq), at 571,591 to 571,692
ylcH
b0547: ybcN - DLP12 prophage; predicted protein (NCBI), at 571,689 to 572,144
ybcN
b0548: ninE - DLP12 prophage; conserved protein (NCBI), at 572,144 to 572,314
ninE
b0549: ybcO - DLP12 prophage; predicted protein (NCBI), at 572,307 to 572,597
ybcO
b0550: rusA - DLP12 prophage; endonuclease RUS (NCBI), at 572,594 to 572,956
rusA
b4509: ylcG - DLP12 prophage; predicted protein (NCBI), at 572,953 to 573,093
ylcG
b0551: ybcQ - DLP12 prophage; predicted antitermination protein (NCBI), at 573,179 to 573,562
ybcQ
b0553: nmpC - DLP12 prophage; truncated outer membrane porin (pseudogene) (RefSeq), at 573,752 to 573,809
nmpC
Position (kb) 572
573 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 571.596 kb on + strand at 571.596 kb on - strand at 571.596 kb on - strand at 571.602 kb on - strand, within ylcH at 571.618 kb on + strand, within ylcH at 571.623 kb on - strand, within ylcH at 571.629 kb on + strand, within ylcH at 571.638 kb on - strand, within ylcH at 571.671 kb on + strand, within ylcH at 571.681 kb on + strand, within ylcH at 571.681 kb on + strand, within ylcH at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.682 kb on - strand at 571.687 kb on + strand at 571.694 kb on - strand at 571.695 kb on - strand at 571.752 kb on + strand, within ybcN at 571.752 kb on + strand, within ybcN at 571.909 kb on + strand, within ybcN at 571.931 kb on + strand, within ybcN at 571.937 kb on - strand, within ybcN at 571.939 kb on - strand, within ybcN at 571.939 kb on - strand, within ybcN at 572.055 kb on + strand, within ybcN at 572.068 kb on - strand, within ybcN at 572.179 kb on + strand, within ninE at 572.179 kb on + strand, within ninE at 572.179 kb on + strand, within ninE at 572.195 kb on - strand, within ninE at 572.214 kb on - strand, within ninE at 572.343 kb on - strand, within ybcO at 572.557 kb on - strand, within ybcO at 572.617 kb on + strand at 572.625 kb on - strand at 572.679 kb on - strand, within rusA at 572.679 kb on - strand, within rusA at 572.697 kb on - strand, within rusA at 572.697 kb on - strand, within rusA at 572.780 kb on + strand, within rusA at 572.899 kb on - strand, within rusA at 572.981 kb on - strand, within ylcG at 573.031 kb on + strand, within ylcG at 573.031 kb on + strand, within ylcG at 573.078 kb on - strand, within ylcG at 573.080 kb on + strand at 573.088 kb on - strand at 573.094 kb on + strand at 573.141 kb on + strand at 573.164 kb on + strand at 573.164 kb on + strand at 573.183 kb on + strand at 573.186 kb on + strand at 573.224 kb on + strand, within ybcQ at 573.224 kb on + strand, within ybcQ at 573.231 kb on + strand, within ybcQ at 573.231 kb on + strand, within ybcQ at 573.239 kb on + strand, within ybcQ at 573.242 kb on + strand, within ybcQ at 573.247 kb on - strand, within ybcQ at 573.267 kb on + strand, within ybcQ at 573.267 kb on + strand, within ybcQ at 573.310 kb on + strand, within ybcQ at 573.310 kb on + strand, within ybcQ at 573.331 kb on - strand, within ybcQ at 573.335 kb on + strand, within ybcQ at 573.335 kb on + strand, within ybcQ at 573.343 kb on + strand, within ybcQ at 573.343 kb on + strand, within ybcQ at 573.343 kb on + strand, within ybcQ at 573.344 kb on + strand, within ybcQ at 573.344 kb on + strand, within ybcQ at 573.344 kb on + strand, within ybcQ at 573.358 kb on + strand, within ybcQ at 573.358 kb on + strand, within ybcQ at 573.411 kb on + strand, within ybcQ at 573.423 kb on + strand, within ybcQ at 573.423 kb on + strand, within ybcQ at 573.424 kb on + strand, within ybcQ at 573.430 kb on + strand, within ybcQ at 573.430 kb on + strand, within ybcQ at 573.434 kb on + strand, within ybcQ at 573.434 kb on + strand, within ybcQ at 573.434 kb on + strand, within ybcQ at 573.437 kb on + strand, within ybcQ at 573.437 kb on + strand, within ybcQ at 573.442 kb on + strand, within ybcQ at 573.442 kb on + strand, within ybcQ at 573.442 kb on + strand, within ybcQ at 573.442 kb on + strand, within ybcQ at 573.442 kb on + strand, within ybcQ at 573.442 kb on + strand, within ybcQ at 573.470 kb on + strand, within ybcQ at 573.488 kb on + strand, within ybcQ at 573.512 kb on + strand, within ybcQ at 573.652 kb on - strand at 573.652 kb on - strand at 573.665 kb on - strand at 573.665 kb on - strand at 573.665 kb on - strand at 573.665 kb on - strand at 573.665 kb on - strand at 573.678 kb on - strand at 573.678 kb on - strand at 573.678 kb on - strand at 573.684 kb on + strand at 573.687 kb on - strand at 573.687 kb on - strand at 573.691 kb on - strand at 573.706 kb on - strand at 573.707 kb on - strand at 573.707 kb on - strand at 573.712 kb on - strand at 573.731 kb on - strand at 573.731 kb on - strand at 573.731 kb on - strand at 573.731 kb on - strand at 573.758 kb on - strand, within nmpC at 573.758 kb on - strand, within nmpC at 573.758 kb on - strand, within nmpC at 573.767 kb on - strand, within nmpC at 573.767 kb on - strand, within nmpC at 573.767 kb on - strand, within nmpC at 573.767 kb on - strand, within nmpC at 573.767 kb on - strand, within nmpC at 573.768 kb on - strand, within nmpC at 573.777 kb on - strand, within nmpC at 573.777 kb on - strand, within nmpC at 573.777 kb on - strand, within nmpC at 573.777 kb on - strand, within nmpC at 573.777 kb on - strand, within nmpC at 573.777 kb on - strand, within nmpC at 573.777 kb on - strand, within nmpC at 573.778 kb on - strand, within nmpC at 573.778 kb on - strand, within nmpC at 573.780 kb on - strand, within nmpC at 573.780 kb on - strand, within nmpC at 573.792 kb on + strand, within nmpC at 573.792 kb on + strand, within nmpC at 573.795 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.801 kb on - strand, within nmpC at 573.803 kb on - strand, within nmpC at 573.816 kb on - strand at 573.827 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM and Betaine 100 uM remove 571,596 + -1.1 571,596 - -0.6 571,596 - +0.4 571,602 - ylcH b4588 0.11 +0.2 571,618 + ylcH b4588 0.26 -0.8 571,623 - ylcH b4588 0.31 -0.0 571,629 + ylcH b4588 0.37 -0.3 571,638 - ylcH b4588 0.46 -0.3 571,671 + ylcH b4588 0.78 -0.5 571,681 + ylcH b4588 0.88 +0.1 571,681 + ylcH b4588 0.88 -0.1 571,682 - -0.4 571,682 - -0.7 571,682 - -1.8 571,682 - +0.6 571,682 - -0.2 571,682 - +0.0 571,682 - +1.5 571,687 + -0.1 571,694 - +0.5 571,695 - +0.4 571,752 + ybcN b0547 0.14 +0.1 571,752 + ybcN b0547 0.14 -0.6 571,909 + ybcN b0547 0.48 +0.3 571,931 + ybcN b0547 0.53 -0.4 571,937 - ybcN b0547 0.54 -0.3 571,939 - ybcN b0547 0.55 -0.3 571,939 - ybcN b0547 0.55 +0.5 572,055 + ybcN b0547 0.80 -0.6 572,068 - ybcN b0547 0.83 +0.4 572,179 + ninE b0548 0.20 +0.7 572,179 + ninE b0548 0.20 +0.5 572,179 + ninE b0548 0.20 +0.0 572,195 - ninE b0548 0.30 -0.4 572,214 - ninE b0548 0.41 -0.1 572,343 - ybcO b0549 0.12 -0.5 572,557 - ybcO b0549 0.86 +0.8 572,617 + +0.0 572,625 - -1.0 572,679 - rusA b0550 0.23 +1.1 572,679 - rusA b0550 0.23 +0.5 572,697 - rusA b0550 0.28 +2.0 572,697 - rusA b0550 0.28 -1.3 572,780 + rusA b0550 0.51 -0.2 572,899 - rusA b0550 0.84 -1.6 572,981 - ylcG b4509 0.20 -1.2 573,031 + ylcG b4509 0.55 -0.8 573,031 + ylcG b4509 0.55 -0.1 573,078 - ylcG b4509 0.89 +0.9 573,080 + +0.2 573,088 - -0.1 573,094 + +0.1 573,141 + -0.7 573,164 + +0.6 573,164 + +0.7 573,183 + +1.0 573,186 + -0.3 573,224 + ybcQ b0551 0.12 +0.5 573,224 + ybcQ b0551 0.12 -1.5 573,231 + ybcQ b0551 0.14 -0.1 573,231 + ybcQ b0551 0.14 -0.9 573,239 + ybcQ b0551 0.16 -0.4 573,242 + ybcQ b0551 0.16 +0.3 573,247 - ybcQ b0551 0.18 +1.5 573,267 + ybcQ b0551 0.23 +0.4 573,267 + ybcQ b0551 0.23 +0.4 573,310 + ybcQ b0551 0.34 +1.4 573,310 + ybcQ b0551 0.34 +0.0 573,331 - ybcQ b0551 0.40 +1.5 573,335 + ybcQ b0551 0.41 +0.6 573,335 + ybcQ b0551 0.41 +0.9 573,343 + ybcQ b0551 0.43 +0.1 573,343 + ybcQ b0551 0.43 -0.4 573,343 + ybcQ b0551 0.43 -0.3 573,344 + ybcQ b0551 0.43 -0.2 573,344 + ybcQ b0551 0.43 -0.4 573,344 + ybcQ b0551 0.43 +0.0 573,358 + ybcQ b0551 0.47 +0.7 573,358 + ybcQ b0551 0.47 -0.5 573,411 + ybcQ b0551 0.60 -0.4 573,423 + ybcQ b0551 0.64 -0.0 573,423 + ybcQ b0551 0.64 +0.8 573,424 + ybcQ b0551 0.64 +0.2 573,430 + ybcQ b0551 0.65 +0.4 573,430 + ybcQ b0551 0.65 -0.5 573,434 + ybcQ b0551 0.66 +0.1 573,434 + ybcQ b0551 0.66 +0.1 573,434 + ybcQ b0551 0.66 +0.3 573,437 + ybcQ b0551 0.67 -0.2 573,437 + ybcQ b0551 0.67 -1.6 573,442 + ybcQ b0551 0.68 +0.2 573,442 + ybcQ b0551 0.68 +0.4 573,442 + ybcQ b0551 0.68 +0.6 573,442 + ybcQ b0551 0.68 +0.4 573,442 + ybcQ b0551 0.68 +0.4 573,442 + ybcQ b0551 0.68 +0.5 573,470 + ybcQ b0551 0.76 -0.1 573,488 + ybcQ b0551 0.80 -0.7 573,512 + ybcQ b0551 0.87 -1.1 573,652 - -1.7 573,652 - +0.4 573,665 - +1.9 573,665 - +0.5 573,665 - -0.1 573,665 - -0.6 573,665 - -0.1 573,678 - -0.0 573,678 - +0.3 573,678 - +0.1 573,684 + +0.9 573,687 - -0.0 573,687 - -0.7 573,691 - -0.1 573,706 - +0.7 573,707 - -0.1 573,707 - -0.2 573,712 - -0.7 573,731 - -1.5 573,731 - +0.8 573,731 - -0.0 573,731 - +0.9 573,758 - nmpC b0553 0.10 +0.1 573,758 - nmpC b0553 0.10 +0.6 573,758 - nmpC b0553 0.10 -0.5 573,767 - nmpC b0553 0.26 +0.0 573,767 - nmpC b0553 0.26 +0.5 573,767 - nmpC b0553 0.26 +0.3 573,767 - nmpC b0553 0.26 +0.1 573,767 - nmpC b0553 0.26 +0.1 573,768 - nmpC b0553 0.28 +0.0 573,777 - nmpC b0553 0.43 +0.0 573,777 - nmpC b0553 0.43 -0.2 573,777 - nmpC b0553 0.43 -0.3 573,777 - nmpC b0553 0.43 +0.0 573,777 - nmpC b0553 0.43 +0.5 573,777 - nmpC b0553 0.43 +0.6 573,777 - nmpC b0553 0.43 +0.0 573,778 - nmpC b0553 0.45 +0.3 573,778 - nmpC b0553 0.45 +0.1 573,780 - nmpC b0553 0.48 -0.0 573,780 - nmpC b0553 0.48 +0.5 573,792 + nmpC b0553 0.69 +1.5 573,792 + nmpC b0553 0.69 +1.5 573,795 - nmpC b0553 0.74 +0.1 573,801 - nmpC b0553 0.84 -1.7 573,801 - nmpC b0553 0.84 +0.3 573,801 - nmpC b0553 0.84 -0.1 573,801 - nmpC b0553 0.84 -0.9 573,801 - nmpC b0553 0.84 +0.1 573,801 - nmpC b0553 0.84 +0.3 573,801 - nmpC b0553 0.84 -4.2 573,801 - nmpC b0553 0.84 -0.1 573,801 - nmpC b0553 0.84 +0.5 573,801 - nmpC b0553 0.84 -0.7 573,801 - nmpC b0553 0.84 -0.2 573,801 - nmpC b0553 0.84 +0.1 573,803 - nmpC b0553 0.88 -0.3 573,816 - +0.2 573,827 - +0.2
Or see this region's nucleotide sequence