Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bglJ and fhuF are separated by 37 nucleotides fhuF and yjjZ are separated by 140 nucleotides yjjZ and leuV are separated by 38 nucleotides leuV and leuP are separated by 34 nucleotides leuP and leuQ are separated by 28 nucleotides leuQ and rsmC are separated by 267 nucleotides
b4366: bglJ - DNA-binding transcriptional activator for silent bgl operon, requires the bglJ4 allele to function; LuxR family (NCBI), at 4,602,183 to 4,602,860
bglJ
b4367: fhuF - ferric iron reductase involved in ferric hydroximate transport (NCBI), at 4,602,898 to 4,603,686
fhuF
b4567: yjjZ - hypothetical protein (NCBI), at 4,603,827 to 4,604,063
yjjZ
b4368: leuV - tRNA-Leu (NCBI), at 4,604,102 to 4,604,188
leuV
b4369: leuP - tRNA-Leu (NCBI), at 4,604,223 to 4,604,309
leuP
b4370: leuQ - tRNA-Leu (NCBI), at 4,604,338 to 4,604,424
leuQ
b4371: rsmC - 16S ribosomal RNA m2G1207 methyltransferase (NCBI), at 4,604,692 to 4,605,723
rsmC
Position (kb)
4603
4604
4605 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4602.835 kb on + strand at 4602.849 kb on - strand at 4602.854 kb on + strand at 4602.860 kb on - strand at 4602.877 kb on - strand at 4602.877 kb on - strand at 4602.877 kb on - strand at 4602.894 kb on + strand at 4602.894 kb on + strand at 4602.904 kb on - strand at 4602.930 kb on - strand at 4602.932 kb on - strand at 4602.938 kb on - strand at 4602.953 kb on + strand at 4602.969 kb on + strand at 4602.969 kb on + strand at 4602.970 kb on - strand at 4602.972 kb on - strand at 4602.972 kb on - strand at 4602.987 kb on + strand, within fhuF at 4602.987 kb on + strand, within fhuF at 4602.987 kb on + strand, within fhuF at 4602.995 kb on - strand, within fhuF at 4602.995 kb on - strand, within fhuF at 4603.006 kb on - strand, within fhuF at 4603.015 kb on - strand, within fhuF at 4603.015 kb on - strand, within fhuF at 4603.068 kb on - strand, within fhuF at 4603.076 kb on + strand, within fhuF at 4603.100 kb on + strand, within fhuF at 4603.102 kb on - strand, within fhuF at 4603.103 kb on - strand, within fhuF at 4603.103 kb on - strand, within fhuF at 4603.165 kb on + strand, within fhuF at 4603.175 kb on - strand, within fhuF at 4603.204 kb on + strand, within fhuF at 4603.207 kb on + strand, within fhuF at 4603.207 kb on + strand, within fhuF at 4603.232 kb on + strand, within fhuF at 4603.312 kb on + strand, within fhuF at 4603.360 kb on - strand, within fhuF at 4603.369 kb on + strand, within fhuF at 4603.385 kb on - strand, within fhuF at 4603.385 kb on - strand, within fhuF at 4603.388 kb on + strand, within fhuF at 4603.388 kb on + strand, within fhuF at 4603.393 kb on + strand, within fhuF at 4603.405 kb on + strand, within fhuF at 4603.519 kb on + strand, within fhuF at 4603.847 kb on - strand at 4603.872 kb on + strand, within yjjZ at 4603.882 kb on + strand, within yjjZ at 4603.927 kb on - strand, within yjjZ at 4603.927 kb on - strand, within yjjZ at 4603.935 kb on - strand, within yjjZ at 4603.937 kb on + strand, within yjjZ at 4604.119 kb on - strand, within leuV at 4604.280 kb on - strand, within leuP at 4604.280 kb on - strand, within leuP at 4604.377 kb on - strand, within leuQ at 4604.377 kb on - strand, within leuQ at 4604.381 kb on - strand, within leuQ at 4604.387 kb on + strand, within leuQ at 4604.410 kb on + strand, within leuQ at 4604.467 kb on + strand at 4604.467 kb on + strand at 4604.467 kb on + strand at 4604.508 kb on - strand at 4604.618 kb on - strand at 4604.653 kb on - strand at 4604.732 kb on + strand at 4604.732 kb on + strand at 4604.745 kb on + strand at 4604.758 kb on + strand at 4604.851 kb on + strand, within rsmC at 4604.856 kb on + strand, within rsmC at 4604.884 kb on + strand, within rsmC at 4604.884 kb on + strand, within rsmC at 4604.927 kb on - strand, within rsmC at 4604.927 kb on - strand, within rsmC at 4605.041 kb on + strand, within rsmC
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM remove 4,602,835 + +0.8 4,602,849 - -0.7 4,602,854 + +0.5 4,602,860 - +0.2 4,602,877 - -0.0 4,602,877 - +0.6 4,602,877 - -0.0 4,602,894 + -0.9 4,602,894 + -1.0 4,602,904 - -0.6 4,602,930 - -0.0 4,602,932 - -0.2 4,602,938 - +0.5 4,602,953 + -0.4 4,602,969 + +0.7 4,602,969 + -0.6 4,602,970 - -0.5 4,602,972 - -0.4 4,602,972 - -0.1 4,602,987 + fhuF b4367 0.11 -0.5 4,602,987 + fhuF b4367 0.11 +0.9 4,602,987 + fhuF b4367 0.11 -0.9 4,602,995 - fhuF b4367 0.12 -0.2 4,602,995 - fhuF b4367 0.12 -0.4 4,603,006 - fhuF b4367 0.14 +0.3 4,603,015 - fhuF b4367 0.15 +0.3 4,603,015 - fhuF b4367 0.15 +0.5 4,603,068 - fhuF b4367 0.22 +0.3 4,603,076 + fhuF b4367 0.23 +0.3 4,603,100 + fhuF b4367 0.26 +0.5 4,603,102 - fhuF b4367 0.26 -0.6 4,603,103 - fhuF b4367 0.26 -0.1 4,603,103 - fhuF b4367 0.26 -0.4 4,603,165 + fhuF b4367 0.34 +0.1 4,603,175 - fhuF b4367 0.35 +0.4 4,603,204 + fhuF b4367 0.39 +0.2 4,603,207 + fhuF b4367 0.39 -1.2 4,603,207 + fhuF b4367 0.39 +0.0 4,603,232 + fhuF b4367 0.42 -0.1 4,603,312 + fhuF b4367 0.52 +0.5 4,603,360 - fhuF b4367 0.59 +0.2 4,603,369 + fhuF b4367 0.60 -0.9 4,603,385 - fhuF b4367 0.62 +0.2 4,603,385 - fhuF b4367 0.62 +0.3 4,603,388 + fhuF b4367 0.62 +0.4 4,603,388 + fhuF b4367 0.62 -0.2 4,603,393 + fhuF b4367 0.63 -0.3 4,603,405 + fhuF b4367 0.64 -2.9 4,603,519 + fhuF b4367 0.79 +1.2 4,603,847 - -0.0 4,603,872 + yjjZ b4567 0.19 +0.5 4,603,882 + yjjZ b4567 0.23 +0.6 4,603,927 - yjjZ b4567 0.42 +0.4 4,603,927 - yjjZ b4567 0.42 +0.7 4,603,935 - yjjZ b4567 0.46 +0.3 4,603,937 + yjjZ b4567 0.46 -0.1 4,604,119 - leuV b4368 0.20 +1.9 4,604,280 - leuP b4369 0.66 +0.2 4,604,280 - leuP b4369 0.66 +0.4 4,604,377 - leuQ b4370 0.45 -0.1 4,604,377 - leuQ b4370 0.45 +0.6 4,604,381 - leuQ b4370 0.49 -0.2 4,604,387 + leuQ b4370 0.56 +1.2 4,604,410 + leuQ b4370 0.83 +1.7 4,604,467 + -1.5 4,604,467 + -0.7 4,604,467 + -0.2 4,604,508 - +0.6 4,604,618 - +0.1 4,604,653 - -0.0 4,604,732 + +0.1 4,604,732 + -0.9 4,604,745 + -0.1 4,604,758 + +0.6 4,604,851 + rsmC b4371 0.15 +0.2 4,604,856 + rsmC b4371 0.16 +0.5 4,604,884 + rsmC b4371 0.19 -0.4 4,604,884 + rsmC b4371 0.19 +0.0 4,604,927 - rsmC b4371 0.23 +0.4 4,604,927 - rsmC b4371 0.23 +0.8 4,605,041 + rsmC b4371 0.34 +0.8
Or see this region's nucleotide sequence