Strain Fitness in Escherichia coli BW25113 around b1977

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntserU and b1976 are separated by 93 nucleotidesb1976 and asnT are separated by 100 nucleotidesasnT and yeeJ are separated by 313 nucleotides b1975: serU - tRNA-Ser (NCBI), at 2,041,492 to 2,041,581 serU b1976: b1976 - orf, hypothetical protein (VIMSS), at 2,041,675 to 2,042,472 b1976 b1977: asnT - tRNA-Asn (NCBI), at 2,042,573 to 2,042,648 asnT b1978: yeeJ - adhesin (RefSeq), at 2,042,962 to 2,050,038 yeeJ Position (kb) 2042 2043Strain fitness (log2 ratio) -1 0 1 2 3at 2041.871 kb on + strand, within b1976at 2041.999 kb on - strand, within b1976at 2042.029 kb on + strand, within b1976at 2042.037 kb on - strand, within b1976at 2042.042 kb on + strand, within b1976at 2042.055 kb on - strand, within b1976at 2042.095 kb on + strand, within b1976at 2042.160 kb on - strand, within b1976at 2042.160 kb on - strand, within b1976at 2042.403 kb on + strandat 2042.418 kb on - strandat 2042.434 kb on + strandat 2042.434 kb on + strandat 2042.619 kb on + strand, within asnTat 2042.619 kb on + strand, within asnTat 2042.747 kb on + strandat 2042.824 kb on - strandat 2042.917 kb on + strandat 2042.917 kb on + strandat 2042.923 kb on - strandat 2042.923 kb on - strandat 2042.923 kb on - strandat 2042.925 kb on - strandat 2042.963 kb on + strandat 2042.963 kb on + strandat 2042.972 kb on - strandat 2043.094 kb on + strandat 2043.094 kb on + strandat 2043.118 kb on + strandat 2043.176 kb on + strandat 2043.184 kb on - strandat 2043.319 kb on - strandat 2043.354 kb on + strandat 2043.432 kb on - strandat 2043.524 kb on + strandat 2043.524 kb on + strandat 2043.564 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM
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2,041,871 + b1976 b1976 0.25 -0.6
2,041,999 - b1976 b1976 0.41 +0.1
2,042,029 + b1976 b1976 0.44 -0.1
2,042,037 - b1976 b1976 0.45 -0.9
2,042,042 + b1976 b1976 0.46 +0.3
2,042,055 - b1976 b1976 0.48 -0.7
2,042,095 + b1976 b1976 0.53 +0.2
2,042,160 - b1976 b1976 0.61 +3.2
2,042,160 - b1976 b1976 0.61 +0.9
2,042,403 + -0.2
2,042,418 - +0.7
2,042,434 + -0.5
2,042,434 + +0.2
2,042,619 + asnT b1977 0.61 +0.3
2,042,619 + asnT b1977 0.61 +0.9
2,042,747 + -1.0
2,042,824 - +1.9
2,042,917 + -0.4
2,042,917 + -0.5
2,042,923 - +0.4
2,042,923 - +0.5
2,042,923 - -0.4
2,042,925 - +0.7
2,042,963 + -0.7
2,042,963 + +0.2
2,042,972 - +0.5
2,043,094 + -0.8
2,043,094 + +0.8
2,043,118 + -0.8
2,043,176 + -0.9
2,043,184 - -0.0
2,043,319 - +0.5
2,043,354 + +0.4
2,043,432 - +0.9
2,043,524 + +0.1
2,043,524 + -0.8
2,043,564 + +0.2

Or see this region's nucleotide sequence