Strain Fitness in Escherichia coli BW25113 around b2309

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisM and hisQ overlap by 4 nucleotideshisQ and hisJ are separated by 89 nucleotideshisJ and argT are separated by 220 nucleotides b2307: hisM - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,422,539 to 2,423,255 hisM b2308: hisQ - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,423,252 to 2,423,938 hisQ b2309: hisJ - histidine/lysine/arginine/ornithine transporter subunit (NCBI), at 2,424,028 to 2,424,810 hisJ b2310: argT - lysine/arginine/ornithine transporter subunit (NCBI), at 2,425,031 to 2,425,813 argT Position (kb) 2424 2425Strain fitness (log2 ratio) -1 0 1at 2423.038 kb on + strand, within hisMat 2423.041 kb on - strand, within hisMat 2423.145 kb on + strand, within hisMat 2423.145 kb on + strand, within hisMat 2423.199 kb on - strandat 2423.199 kb on - strandat 2423.233 kb on - strandat 2423.240 kb on - strandat 2423.240 kb on - strandat 2423.265 kb on - strandat 2423.265 kb on - strandat 2423.267 kb on + strandat 2423.280 kb on + strandat 2423.280 kb on + strandat 2423.310 kb on - strandat 2423.310 kb on - strandat 2423.346 kb on + strand, within hisQat 2423.421 kb on - strand, within hisQat 2423.426 kb on + strand, within hisQat 2423.490 kb on + strand, within hisQat 2423.566 kb on + strand, within hisQat 2423.572 kb on - strand, within hisQat 2423.641 kb on + strand, within hisQat 2423.670 kb on + strand, within hisQat 2423.696 kb on + strand, within hisQat 2423.696 kb on + strand, within hisQat 2423.702 kb on - strand, within hisQat 2423.702 kb on - strand, within hisQat 2423.752 kb on - strand, within hisQat 2423.779 kb on + strand, within hisQat 2423.786 kb on - strand, within hisQat 2423.830 kb on - strand, within hisQat 2423.830 kb on - strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.866 kb on + strand, within hisQat 2423.940 kb on + strandat 2424.014 kb on - strandat 2424.158 kb on - strand, within hisJat 2424.158 kb on - strand, within hisJat 2424.158 kb on - strand, within hisJat 2424.239 kb on - strand, within hisJat 2424.383 kb on + strand, within hisJat 2424.384 kb on - strand, within hisJat 2424.406 kb on - strand, within hisJat 2424.863 kb on - strandat 2425.153 kb on + strand, within argTat 2425.153 kb on + strand, within argTat 2425.161 kb on - strand, within argTat 2425.161 kb on - strand, within argTat 2425.253 kb on + strand, within argTat 2425.280 kb on + strand, within argTat 2425.286 kb on - strand, within argTat 2425.319 kb on - strand, within argTat 2425.339 kb on - strand, within argTat 2425.353 kb on - strand, within argTat 2425.353 kb on - strand, within argTat 2425.387 kb on - strand, within argTat 2425.387 kb on - strand, within argTat 2425.402 kb on - strand, within argTat 2425.543 kb on + strand, within argTat 2425.702 kb on + strand, within argTat 2425.705 kb on - strand, within argTat 2425.732 kb on - strand, within argTat 2425.742 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM
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2,423,038 + hisM b2307 0.70 -0.3
2,423,041 - hisM b2307 0.70 +0.6
2,423,145 + hisM b2307 0.85 -0.5
2,423,145 + hisM b2307 0.85 +0.9
2,423,199 - +0.2
2,423,199 - +0.5
2,423,233 - -0.2
2,423,240 - +0.6
2,423,240 - +1.4
2,423,265 - +0.3
2,423,265 - +0.2
2,423,267 + -0.0
2,423,280 + -0.8
2,423,280 + +0.4
2,423,310 - +0.2
2,423,310 - -0.2
2,423,346 + hisQ b2308 0.14 +0.1
2,423,421 - hisQ b2308 0.25 +0.1
2,423,426 + hisQ b2308 0.25 +0.2
2,423,490 + hisQ b2308 0.35 -0.9
2,423,566 + hisQ b2308 0.46 +0.5
2,423,572 - hisQ b2308 0.47 +0.0
2,423,641 + hisQ b2308 0.57 +0.4
2,423,670 + hisQ b2308 0.61 -0.1
2,423,696 + hisQ b2308 0.65 -0.2
2,423,696 + hisQ b2308 0.65 +0.5
2,423,702 - hisQ b2308 0.66 -0.3
2,423,702 - hisQ b2308 0.66 +0.1
2,423,752 - hisQ b2308 0.73 -0.2
2,423,779 + hisQ b2308 0.77 -0.5
2,423,786 - hisQ b2308 0.78 -0.2
2,423,830 - hisQ b2308 0.84 +0.5
2,423,830 - hisQ b2308 0.84 +0.0
2,423,866 + hisQ b2308 0.89 -0.1
2,423,866 + hisQ b2308 0.89 +0.7
2,423,866 + hisQ b2308 0.89 +0.4
2,423,866 + hisQ b2308 0.89 -0.5
2,423,866 + hisQ b2308 0.89 +0.2
2,423,940 + +0.1
2,424,014 - +0.3
2,424,158 - hisJ b2309 0.17 +0.6
2,424,158 - hisJ b2309 0.17 +0.8
2,424,158 - hisJ b2309 0.17 -0.0
2,424,239 - hisJ b2309 0.27 +0.4
2,424,383 + hisJ b2309 0.45 -0.2
2,424,384 - hisJ b2309 0.45 +0.4
2,424,406 - hisJ b2309 0.48 +1.0
2,424,863 - +0.1
2,425,153 + argT b2310 0.16 +0.2
2,425,153 + argT b2310 0.16 +0.4
2,425,161 - argT b2310 0.17 +0.3
2,425,161 - argT b2310 0.17 +0.0
2,425,253 + argT b2310 0.28 -0.3
2,425,280 + argT b2310 0.32 -0.1
2,425,286 - argT b2310 0.33 -0.7
2,425,319 - argT b2310 0.37 +0.3
2,425,339 - argT b2310 0.39 -0.5
2,425,353 - argT b2310 0.41 -0.0
2,425,353 - argT b2310 0.41 +0.3
2,425,387 - argT b2310 0.45 +0.2
2,425,387 - argT b2310 0.45 -1.3
2,425,402 - argT b2310 0.47 +0.4
2,425,543 + argT b2310 0.65 +0.3
2,425,702 + argT b2310 0.86 +0.1
2,425,705 - argT b2310 0.86 +0.1
2,425,732 - argT b2310 0.90 -0.9
2,425,742 - -0.0

Or see this region's nucleotide sequence