Strain Fitness in Escherichia coli BW25113 around b2083

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyegQ and cyaR are separated by 84 nucleotidescyaR and ogrK are separated by 101 nucleotidesogrK and yegZ are separated by 81 nucleotidesyegZ and b2085 are separated by 174 nucleotidesb2085 and yegS are separated by 405 nucleotides b2081: yegQ - predicted peptidase (NCBI), at 2,163,692 to 2,165,053 yegQ b4438: cyaR - sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq), at 2,165,138 to 2,165,224 cyaR b2082: ogrK - DNA-binding transcriptional regulator prophage P2 remnant (NCBI), at 2,165,326 to 2,165,544 ogrK b2083: yegZ - orf, hypothetical protein (VIMSS), at 2,165,626 to 2,165,838 yegZ b2085: b2085 - orf, hypothetical protein (VIMSS), at 2,166,013 to 2,166,330 b2085 b2086: yegS - hypothetical protein (NCBI), at 2,166,736 to 2,167,635 yegS Position (kb) 2165 2166Strain fitness (log2 ratio) -2 -1 0 1 2at 2164.653 kb on + strand, within yegQat 2164.657 kb on + strand, within yegQat 2164.713 kb on + strand, within yegQat 2164.713 kb on + strand, within yegQat 2164.798 kb on + strand, within yegQat 2164.927 kb on - strandat 2164.950 kb on + strandat 2165.031 kb on + strandat 2165.109 kb on - strandat 2165.109 kb on - strandat 2165.177 kb on - strand, within cyaRat 2165.184 kb on + strand, within cyaRat 2165.184 kb on + strand, within cyaRat 2165.383 kb on - strand, within ogrKat 2165.423 kb on - strand, within ogrKat 2165.658 kb on - strand, within yegZat 2165.732 kb on + strand, within yegZat 2165.732 kb on + strand, within yegZat 2165.872 kb on + strandat 2165.894 kb on - strandat 2165.907 kb on + strandat 2165.929 kb on + strandat 2165.929 kb on + strandat 2165.964 kb on + strandat 2165.968 kb on + strandat 2166.018 kb on + strandat 2166.018 kb on + strandat 2166.023 kb on + strandat 2166.047 kb on + strand, within b2085at 2166.047 kb on + strand, within b2085at 2166.047 kb on - strand, within b2085at 2166.047 kb on - strand, within b2085at 2166.052 kb on - strand, within b2085at 2166.054 kb on + strand, within b2085at 2166.054 kb on + strand, within b2085at 2166.054 kb on + strand, within b2085at 2166.054 kb on + strand, within b2085at 2166.054 kb on + strand, within b2085at 2166.054 kb on + strand, within b2085at 2166.055 kb on + strand, within b2085at 2166.062 kb on - strand, within b2085at 2166.102 kb on + strand, within b2085at 2166.102 kb on + strand, within b2085at 2166.155 kb on + strand, within b2085at 2166.188 kb on + strand, within b2085at 2166.188 kb on + strand, within b2085at 2166.588 kb on - strandat 2166.634 kb on - strandat 2166.634 kb on - strandat 2166.635 kb on - strandat 2166.636 kb on - strandat 2166.661 kb on - strandat 2166.693 kb on + strandat 2166.693 kb on + strandat 2166.698 kb on + strandat 2166.708 kb on - strandat 2166.713 kb on - strandat 2166.723 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM
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2,164,653 + yegQ b2081 0.71 -0.6
2,164,657 + yegQ b2081 0.71 +0.6
2,164,713 + yegQ b2081 0.75 +0.6
2,164,713 + yegQ b2081 0.75 +0.1
2,164,798 + yegQ b2081 0.81 +0.2
2,164,927 - -0.5
2,164,950 + +0.8
2,165,031 + -1.6
2,165,109 - -0.6
2,165,109 - +1.7
2,165,177 - cyaR b4438 0.45 -0.9
2,165,184 + cyaR b4438 0.53 +0.5
2,165,184 + cyaR b4438 0.53 +1.1
2,165,383 - ogrK b2082 0.26 +0.4
2,165,423 - ogrK b2082 0.44 -0.2
2,165,658 - yegZ b2083 0.15 +0.5
2,165,732 + yegZ b2083 0.50 +0.7
2,165,732 + yegZ b2083 0.50 +0.1
2,165,872 + +0.6
2,165,894 - +0.6
2,165,907 + -0.1
2,165,929 + -0.2
2,165,929 + +0.7
2,165,964 + -0.7
2,165,968 + -1.6
2,166,018 + -2.0
2,166,018 + +1.2
2,166,023 + +2.7
2,166,047 + b2085 b2085 0.11 +0.4
2,166,047 + b2085 b2085 0.11 +0.5
2,166,047 - b2085 b2085 0.11 +1.0
2,166,047 - b2085 b2085 0.11 -1.0
2,166,052 - b2085 b2085 0.12 +1.4
2,166,054 + b2085 b2085 0.13 -1.2
2,166,054 + b2085 b2085 0.13 +1.0
2,166,054 + b2085 b2085 0.13 +0.1
2,166,054 + b2085 b2085 0.13 -0.9
2,166,054 + b2085 b2085 0.13 +0.4
2,166,054 + b2085 b2085 0.13 +1.1
2,166,055 + b2085 b2085 0.13 +1.0
2,166,062 - b2085 b2085 0.15 +2.6
2,166,102 + b2085 b2085 0.28 -1.2
2,166,102 + b2085 b2085 0.28 -0.7
2,166,155 + b2085 b2085 0.45 +0.4
2,166,188 + b2085 b2085 0.55 +2.0
2,166,188 + b2085 b2085 0.55 -0.0
2,166,588 - +1.6
2,166,634 - -1.7
2,166,634 - +2.0
2,166,635 - -2.0
2,166,636 - -1.0
2,166,661 - +0.7
2,166,693 + -0.6
2,166,693 + -0.0
2,166,698 + -0.0
2,166,708 - -0.7
2,166,713 - -0.0
2,166,723 - -0.7

Or see this region's nucleotide sequence