Strain Fitness in Escherichia coli BW25113 around b1888

Experiment: RCH2_defined_1percent_glucose with Chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttar and cheW are separated by 144 nucleotidescheW and cheA are separated by 20 nucleotidescheA and motB are separated by 4 nucleotidesmotB and motA overlap by 4 nucleotides b1886: tar - methyl-accepting chemotaxis protein II (NCBI), at 1,969,054 to 1,970,715 tar b1887: cheW - purine-binding chemotaxis protein (NCBI), at 1,970,860 to 1,971,363 cheW b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348 cheA b1889: motB - flagellar motor protein MotB (NCBI), at 1,973,353 to 1,974,279 motB b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163 motA Position (kb) 1971 1972 1973 1974Strain fitness (log2 ratio) -2 -1 0 1at 1970.390 kb on - strand, within tarat 1970.488 kb on - strand, within tarat 1970.489 kb on + strand, within tarat 1970.497 kb on + strand, within tarat 1970.502 kb on + strand, within tarat 1970.602 kb on + strandat 1970.656 kb on - strandat 1970.666 kb on + strandat 1970.676 kb on + strandat 1970.765 kb on - strandat 1970.884 kb on + strandat 1970.989 kb on - strand, within cheWat 1971.037 kb on - strand, within cheWat 1971.075 kb on + strand, within cheWat 1971.116 kb on + strand, within cheWat 1971.129 kb on - strand, within cheWat 1971.140 kb on - strand, within cheWat 1971.165 kb on + strand, within cheWat 1971.165 kb on + strand, within cheWat 1971.173 kb on - strand, within cheWat 1971.247 kb on + strand, within cheWat 1971.247 kb on + strand, within cheWat 1971.250 kb on + strand, within cheWat 1971.251 kb on + strand, within cheWat 1971.251 kb on + strand, within cheWat 1971.272 kb on + strand, within cheWat 1971.275 kb on - strand, within cheWat 1971.539 kb on + strandat 1971.750 kb on + strand, within cheAat 1971.957 kb on - strand, within cheAat 1972.064 kb on + strand, within cheAat 1972.278 kb on - strand, within cheAat 1972.498 kb on + strand, within cheAat 1972.631 kb on + strand, within cheAat 1972.777 kb on + strand, within cheAat 1972.859 kb on + strand, within cheAat 1972.874 kb on + strand, within cheAat 1972.963 kb on + strand, within cheAat 1973.015 kb on - strand, within cheAat 1973.103 kb on - strand, within cheAat 1973.279 kb on - strandat 1973.279 kb on - strandat 1973.443 kb on - strandat 1973.443 kb on - strandat 1973.577 kb on - strand, within motBat 1973.595 kb on - strand, within motBat 1973.697 kb on + strand, within motBat 1973.846 kb on - strand, within motBat 1973.850 kb on - strand, within motBat 1974.089 kb on - strand, within motBat 1974.089 kb on - strand, within motBat 1974.197 kb on + strandat 1974.226 kb on + strandat 1974.233 kb on + strandat 1974.233 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 200 mM
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1,970,390 - tar b1886 0.80 -0.3
1,970,488 - tar b1886 0.86 -0.1
1,970,489 + tar b1886 0.86 -0.3
1,970,497 + tar b1886 0.87 +0.4
1,970,502 + tar b1886 0.87 -0.3
1,970,602 + +0.9
1,970,656 - -1.0
1,970,666 + +0.6
1,970,676 + +0.0
1,970,765 - +0.4
1,970,884 + -0.1
1,970,989 - cheW b1887 0.26 +0.1
1,971,037 - cheW b1887 0.35 +0.4
1,971,075 + cheW b1887 0.43 +0.6
1,971,116 + cheW b1887 0.51 -0.2
1,971,129 - cheW b1887 0.53 +0.4
1,971,140 - cheW b1887 0.56 -0.5
1,971,165 + cheW b1887 0.61 +1.0
1,971,165 + cheW b1887 0.61 -2.0
1,971,173 - cheW b1887 0.62 +0.0
1,971,247 + cheW b1887 0.77 +0.1
1,971,247 + cheW b1887 0.77 -0.6
1,971,250 + cheW b1887 0.77 -0.5
1,971,251 + cheW b1887 0.78 +0.7
1,971,251 + cheW b1887 0.78 +0.5
1,971,272 + cheW b1887 0.82 +0.1
1,971,275 - cheW b1887 0.82 -0.1
1,971,539 + +0.5
1,971,750 + cheA b1888 0.19 -0.1
1,971,957 - cheA b1888 0.29 +0.5
1,972,064 + cheA b1888 0.35 +0.4
1,972,278 - cheA b1888 0.45 +0.9
1,972,498 + cheA b1888 0.57 +0.5
1,972,631 + cheA b1888 0.63 +1.6
1,972,777 + cheA b1888 0.71 -0.1
1,972,859 + cheA b1888 0.75 +0.2
1,972,874 + cheA b1888 0.76 -0.2
1,972,963 + cheA b1888 0.80 -0.1
1,973,015 - cheA b1888 0.83 +0.3
1,973,103 - cheA b1888 0.87 +0.1
1,973,279 - -0.1
1,973,279 - +0.5
1,973,443 - +1.4
1,973,443 - +0.2
1,973,577 - motB b1889 0.24 -0.4
1,973,595 - motB b1889 0.26 -0.4
1,973,697 + motB b1889 0.37 +0.2
1,973,846 - motB b1889 0.53 +0.3
1,973,850 - motB b1889 0.54 +0.9
1,974,089 - motB b1889 0.79 -0.0
1,974,089 - motB b1889 0.79 -0.1
1,974,197 + +0.1
1,974,226 + -0.4
1,974,233 + -1.0
1,974,233 + +0.1

Or see this region's nucleotide sequence