Strain Fitness in Escherichia coli BW25113 around b3391

Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaroB and aroK are separated by 56 nucleotidesaroK and hofQ are separated by 400 nucleotideshofQ and yrfA overlap by 89 nucleotidesyrfA and yrfB overlap by 11 nucleotidesyrfB and yrfC overlap by 17 nucleotides b3389: aroB - 3-dehydroquinate synthase (NCBI), at 3,515,420 to 3,516,508 aroB b3390: aroK - shikimate kinase I (NCBI), at 3,516,565 to 3,517,086 aroK b3391: hofQ - predicted fimbrial transporter (NCBI), at 3,517,487 to 3,518,725 hofQ b3392: yrfA - orf, hypothetical protein (VIMSS), at 3,518,637 to 3,519,041 yrfA b3393: yrfB - hypothetical protein (NCBI), at 3,519,031 to 3,519,471 yrfB b3394: yrfC - predicted fimbrial assembly protein (NCBI), at 3,519,455 to 3,519,994 yrfC Position (kb) 3517 3518 3519Strain fitness (log2 ratio) -2 -1 0 1at 3516.496 kb on - strandat 3516.501 kb on - strandat 3516.549 kb on + strandat 3516.554 kb on - strandat 3516.554 kb on - strandat 3516.554 kb on - strandat 3516.626 kb on + strand, within aroKat 3516.661 kb on - strand, within aroKat 3516.793 kb on + strand, within aroKat 3516.801 kb on + strand, within aroKat 3516.801 kb on + strand, within aroKat 3516.858 kb on - strand, within aroKat 3517.041 kb on - strandat 3517.046 kb on + strandat 3517.054 kb on - strandat 3517.066 kb on - strandat 3517.107 kb on - strandat 3517.144 kb on + strandat 3517.152 kb on - strandat 3517.164 kb on + strandat 3517.207 kb on + strandat 3517.224 kb on - strandat 3517.228 kb on + strandat 3517.247 kb on + strandat 3517.283 kb on - strandat 3517.360 kb on - strandat 3517.389 kb on - strandat 3517.426 kb on - strandat 3517.437 kb on + strandat 3517.441 kb on - strandat 3517.441 kb on - strandat 3517.530 kb on - strandat 3517.615 kb on - strand, within hofQat 3517.615 kb on - strand, within hofQat 3517.706 kb on + strand, within hofQat 3517.706 kb on + strand, within hofQat 3517.711 kb on + strand, within hofQat 3517.711 kb on + strand, within hofQat 3517.730 kb on + strand, within hofQat 3517.730 kb on + strand, within hofQat 3517.730 kb on + strand, within hofQat 3517.798 kb on + strand, within hofQat 3517.817 kb on + strand, within hofQat 3517.817 kb on - strand, within hofQat 3517.825 kb on - strand, within hofQat 3517.911 kb on + strand, within hofQat 3517.911 kb on + strand, within hofQat 3517.928 kb on - strand, within hofQat 3518.047 kb on + strand, within hofQat 3518.136 kb on - strand, within hofQat 3518.136 kb on - strand, within hofQat 3518.169 kb on - strand, within hofQat 3518.180 kb on - strand, within hofQat 3518.211 kb on + strand, within hofQat 3518.221 kb on + strand, within hofQat 3518.221 kb on + strand, within hofQat 3518.294 kb on + strand, within hofQat 3518.294 kb on - strand, within hofQat 3518.294 kb on - strand, within hofQat 3518.304 kb on - strand, within hofQat 3518.314 kb on - strand, within hofQat 3518.358 kb on + strand, within hofQat 3518.358 kb on + strand, within hofQat 3518.457 kb on + strand, within hofQat 3518.463 kb on - strand, within hofQat 3518.463 kb on - strand, within hofQat 3518.465 kb on - strand, within hofQat 3518.465 kb on - strand, within hofQat 3518.465 kb on - strand, within hofQat 3518.466 kb on - strand, within hofQat 3518.481 kb on - strand, within hofQat 3518.488 kb on + strand, within hofQat 3518.538 kb on + strand, within hofQat 3518.543 kb on + strand, within hofQat 3518.546 kb on + strand, within hofQat 3518.593 kb on - strand, within hofQat 3518.593 kb on - strand, within hofQat 3518.692 kb on - strandat 3518.692 kb on - strandat 3518.709 kb on - strandat 3518.740 kb on - strand, within yrfAat 3518.740 kb on - strand, within yrfAat 3518.751 kb on - strand, within yrfAat 3518.776 kb on + strand, within yrfAat 3518.786 kb on + strand, within yrfAat 3518.786 kb on + strand, within yrfAat 3518.794 kb on - strand, within yrfAat 3518.794 kb on - strand, within yrfAat 3518.841 kb on + strand, within yrfAat 3518.852 kb on - strand, within yrfAat 3518.883 kb on - strand, within yrfAat 3518.883 kb on - strand, within yrfAat 3518.895 kb on - strand, within yrfAat 3518.895 kb on - strand, within yrfAat 3518.915 kb on + strand, within yrfAat 3518.981 kb on + strand, within yrfAat 3518.989 kb on - strand, within yrfAat 3519.000 kb on + strand, within yrfAat 3519.013 kb on - strandat 3519.013 kb on - strandat 3519.013 kb on - strandat 3519.027 kb on + strandat 3519.027 kb on + strandat 3519.027 kb on + strandat 3519.113 kb on + strand, within yrfBat 3519.123 kb on + strand, within yrfBat 3519.123 kb on + strand, within yrfBat 3519.150 kb on + strand, within yrfBat 3519.189 kb on + strand, within yrfBat 3519.189 kb on + strand, within yrfBat 3519.290 kb on + strand, within yrfBat 3519.292 kb on + strand, within yrfBat 3519.363 kb on - strand, within yrfBat 3519.429 kb on + strandat 3519.471 kb on + strandat 3519.471 kb on + strandat 3519.483 kb on + strandat 3519.509 kb on + strand, within yrfCat 3519.509 kb on + strand, within yrfCat 3519.529 kb on + strand, within yrfCat 3519.556 kb on - strand, within yrfCat 3519.556 kb on - strand, within yrfCat 3519.608 kb on - strand, within yrfCat 3519.609 kb on + strand, within yrfCat 3519.609 kb on + strand, within yrfCat 3519.641 kb on - strand, within yrfC

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM
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3,516,496 - -2.2
3,516,501 - -2.4
3,516,549 + -1.3
3,516,554 - -0.4
3,516,554 - -0.1
3,516,554 - -0.2
3,516,626 + aroK b3390 0.12 -1.7
3,516,661 - aroK b3390 0.18 +0.3
3,516,793 + aroK b3390 0.44 -1.4
3,516,801 + aroK b3390 0.45 -0.9
3,516,801 + aroK b3390 0.45 -0.7
3,516,858 - aroK b3390 0.56 +0.1
3,517,041 - -2.6
3,517,046 + -0.9
3,517,054 - -0.3
3,517,066 - -1.7
3,517,107 - -0.4
3,517,144 + -1.6
3,517,152 - +1.0
3,517,164 + -1.5
3,517,207 + -1.0
3,517,224 - +0.9
3,517,228 + -0.4
3,517,247 + -0.2
3,517,283 - +0.7
3,517,360 - +0.9
3,517,389 - +1.1
3,517,426 - +0.4
3,517,437 + -0.7
3,517,441 - +0.6
3,517,441 - +0.5
3,517,530 - +0.6
3,517,615 - hofQ b3391 0.10 +1.0
3,517,615 - hofQ b3391 0.10 +0.7
3,517,706 + hofQ b3391 0.18 -0.1
3,517,706 + hofQ b3391 0.18 +0.3
3,517,711 + hofQ b3391 0.18 +1.2
3,517,711 + hofQ b3391 0.18 +0.1
3,517,730 + hofQ b3391 0.20 +0.2
3,517,730 + hofQ b3391 0.20 +0.1
3,517,730 + hofQ b3391 0.20 +1.2
3,517,798 + hofQ b3391 0.25 -0.1
3,517,817 + hofQ b3391 0.27 -0.2
3,517,817 - hofQ b3391 0.27 +0.0
3,517,825 - hofQ b3391 0.27 +0.9
3,517,911 + hofQ b3391 0.34 -0.2
3,517,911 + hofQ b3391 0.34 +0.3
3,517,928 - hofQ b3391 0.36 +1.1
3,518,047 + hofQ b3391 0.45 +0.2
3,518,136 - hofQ b3391 0.52 +1.1
3,518,136 - hofQ b3391 0.52 +1.0
3,518,169 - hofQ b3391 0.55 -0.0
3,518,180 - hofQ b3391 0.56 +0.1
3,518,211 + hofQ b3391 0.58 +0.4
3,518,221 + hofQ b3391 0.59 +0.6
3,518,221 + hofQ b3391 0.59 +1.0
3,518,294 + hofQ b3391 0.65 +0.1
3,518,294 - hofQ b3391 0.65 -0.4
3,518,294 - hofQ b3391 0.65 +0.4
3,518,304 - hofQ b3391 0.66 +0.4
3,518,314 - hofQ b3391 0.67 +0.3
3,518,358 + hofQ b3391 0.70 +0.3
3,518,358 + hofQ b3391 0.70 +0.7
3,518,457 + hofQ b3391 0.78 -0.8
3,518,463 - hofQ b3391 0.79 +0.7
3,518,463 - hofQ b3391 0.79 +0.4
3,518,465 - hofQ b3391 0.79 +0.4
3,518,465 - hofQ b3391 0.79 +0.4
3,518,465 - hofQ b3391 0.79 +0.4
3,518,466 - hofQ b3391 0.79 +0.7
3,518,481 - hofQ b3391 0.80 +0.5
3,518,488 + hofQ b3391 0.81 +0.3
3,518,538 + hofQ b3391 0.85 +0.2
3,518,543 + hofQ b3391 0.85 +0.3
3,518,546 + hofQ b3391 0.85 +0.9
3,518,593 - hofQ b3391 0.89 +0.1
3,518,593 - hofQ b3391 0.89 +1.0
3,518,692 - +0.2
3,518,692 - -0.2
3,518,709 - -0.7
3,518,740 - yrfA b3392 0.25 +0.4
3,518,740 - yrfA b3392 0.25 +0.0
3,518,751 - yrfA b3392 0.28 +0.2
3,518,776 + yrfA b3392 0.34 +0.3
3,518,786 + yrfA b3392 0.37 -0.0
3,518,786 + yrfA b3392 0.37 -0.7
3,518,794 - yrfA b3392 0.39 +0.6
3,518,794 - yrfA b3392 0.39 +0.8
3,518,841 + yrfA b3392 0.50 -0.3
3,518,852 - yrfA b3392 0.53 -0.0
3,518,883 - yrfA b3392 0.61 +0.6
3,518,883 - yrfA b3392 0.61 -0.6
3,518,895 - yrfA b3392 0.64 +0.3
3,518,895 - yrfA b3392 0.64 -0.2
3,518,915 + yrfA b3392 0.69 -0.2
3,518,981 + yrfA b3392 0.85 -0.1
3,518,989 - yrfA b3392 0.87 +0.4
3,519,000 + yrfA b3392 0.90 +0.5
3,519,013 - +0.2
3,519,013 - +0.5
3,519,013 - +0.7
3,519,027 + -0.2
3,519,027 + -0.1
3,519,027 + +0.4
3,519,113 + yrfB b3393 0.19 +0.1
3,519,123 + yrfB b3393 0.21 +0.4
3,519,123 + yrfB b3393 0.21 -0.8
3,519,150 + yrfB b3393 0.27 +0.4
3,519,189 + yrfB b3393 0.36 +0.1
3,519,189 + yrfB b3393 0.36 +0.2
3,519,290 + yrfB b3393 0.59 +0.4
3,519,292 + yrfB b3393 0.59 -0.2
3,519,363 - yrfB b3393 0.75 +0.7
3,519,429 + +0.3
3,519,471 + -0.1
3,519,471 + -0.0
3,519,483 + +0.4
3,519,509 + yrfC b3394 0.10 +0.0
3,519,509 + yrfC b3394 0.10 +0.2
3,519,529 + yrfC b3394 0.14 +0.7
3,519,556 - yrfC b3394 0.19 +0.1
3,519,556 - yrfC b3394 0.19 +1.4
3,519,608 - yrfC b3394 0.28 +0.1
3,519,609 + yrfC b3394 0.29 -0.2
3,519,609 + yrfC b3394 0.29 +0.5
3,519,641 - yrfC b3394 0.34 +0.0

Or see this region's nucleotide sequence