Strain Fitness in Escherichia coli BW25113 around b1842

Experiment: RCH2_defined_1percent_glucose with Chloride 100 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyebZ and yobA are separated by 3 nucleotidesyobA and holE are separated by 138 nucleotidesholE and yobB are separated by 101 nucleotidesyobB and exoX are separated by 23 nucleotides b1840: yebZ - predicted inner membrane protein (NCBI), at 1,921,743 to 1,922,615 yebZ b1841: yobA - hypothetical protein (NCBI), at 1,922,619 to 1,922,993 yobA b1842: holE - DNA polymerase III, theta subunit (NCBI), at 1,923,132 to 1,923,362 holE b1843: yobB - hypothetical protein (NCBI), at 1,923,464 to 1,924,120 yobB b1844: exoX - exodeoxyribonuclease X (NCBI), at 1,924,144 to 1,924,806 exoX Position (kb) 1923 1924Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1922.363 kb on - strand, within yebZat 1922.363 kb on - strand, within yebZat 1922.430 kb on + strand, within yebZat 1922.430 kb on + strand, within yebZat 1922.438 kb on + strand, within yebZat 1922.446 kb on - strand, within yebZat 1922.482 kb on - strand, within yebZat 1922.528 kb on + strandat 1922.536 kb on - strandat 1922.642 kb on - strandat 1922.681 kb on + strand, within yobAat 1922.681 kb on + strand, within yobAat 1923.028 kb on + strandat 1923.059 kb on - strandat 1923.246 kb on - strand, within holEat 1923.246 kb on - strand, within holEat 1923.296 kb on + strand, within holEat 1923.364 kb on + strandat 1923.364 kb on + strandat 1923.493 kb on + strandat 1923.493 kb on + strandat 1923.666 kb on - strand, within yobBat 1923.831 kb on - strand, within yobBat 1923.840 kb on - strand, within yobBat 1923.840 kb on - strand, within yobBat 1923.846 kb on + strand, within yobBat 1923.995 kb on + strand, within yobBat 1924.003 kb on - strand, within yobBat 1924.274 kb on + strand, within exoXat 1924.274 kb on + strand, within exoXat 1924.274 kb on - strand, within exoXat 1924.309 kb on - strand, within exoXat 1924.361 kb on - strand, within exoXat 1924.361 kb on - strand, within exoX

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_1percent_glucose with Chloride 100 mM
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1,922,363 - yebZ b1840 0.71 +0.3
1,922,363 - yebZ b1840 0.71 -0.2
1,922,430 + yebZ b1840 0.79 +0.0
1,922,430 + yebZ b1840 0.79 -0.7
1,922,438 + yebZ b1840 0.80 +0.2
1,922,446 - yebZ b1840 0.81 -0.3
1,922,482 - yebZ b1840 0.85 -0.5
1,922,528 + -0.2
1,922,536 - -0.7
1,922,642 - +0.3
1,922,681 + yobA b1841 0.17 +1.1
1,922,681 + yobA b1841 0.17 +0.2
1,923,028 + +0.7
1,923,059 - +0.2
1,923,246 - holE b1842 0.49 +0.5
1,923,246 - holE b1842 0.49 +1.7
1,923,296 + holE b1842 0.71 -0.4
1,923,364 + +1.4
1,923,364 + -0.9
1,923,493 + -0.6
1,923,493 + +0.7
1,923,666 - yobB b1843 0.31 +0.6
1,923,831 - yobB b1843 0.56 -0.1
1,923,840 - yobB b1843 0.57 +0.7
1,923,840 - yobB b1843 0.57 -0.3
1,923,846 + yobB b1843 0.58 +0.0
1,923,995 + yobB b1843 0.81 -0.5
1,924,003 - yobB b1843 0.82 +0.0
1,924,274 + exoX b1844 0.20 -0.3
1,924,274 + exoX b1844 0.20 -0.8
1,924,274 - exoX b1844 0.20 -0.2
1,924,309 - exoX b1844 0.25 +4.4
1,924,361 - exoX b1844 0.33 -1.9
1,924,361 - exoX b1844 0.33 +0.1

Or see this region's nucleotide sequence