Strain Fitness in Pseudomonas simiae WCS417 around PS417_00045

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00040 and PS417_00045 are separated by 182 nucleotidesPS417_00045 and PS417_00050 are separated by 82 nucleotidesPS417_00050 and PS417_00055 are separated by 5 nucleotides PS417_00040: PS417_00040 - ATPase, at 8,436 to 10,715 _00040 PS417_00045: PS417_00045 - glycerol acyltransferase, at 10,898 to 11,668 _00045 PS417_00050: PS417_00050 - histidinol phosphatase, at 11,751 to 12,284 _00050 PS417_00055: PS417_00055 - glycine-tRNA synthetase subunit beta, at 12,290 to 14,344 _00055 Position (kb) 10 11 12Strain fitness (log2 ratio) -3 -2 -1 0 1at 9.935 kb on - strand, within PS417_00040at 9.935 kb on - strand, within PS417_00040at 10.043 kb on + strand, within PS417_00040at 10.175 kb on - strand, within PS417_00040at 10.175 kb on - strand, within PS417_00040at 10.275 kb on - strand, within PS417_00040at 10.275 kb on - strand, within PS417_00040at 10.275 kb on - strand, within PS417_00040at 10.303 kb on + strand, within PS417_00040at 10.323 kb on - strand, within PS417_00040at 10.328 kb on + strand, within PS417_00040at 10.328 kb on + strand, within PS417_00040at 10.434 kb on + strand, within PS417_00040at 10.435 kb on - strand, within PS417_00040at 10.435 kb on - strand, within PS417_00040at 10.472 kb on + strand, within PS417_00040at 10.627 kb on + strandat 10.741 kb on - strandat 10.900 kb on - strandat 10.900 kb on - strandat 11.028 kb on + strand, within PS417_00045at 11.679 kb on - strandat 11.679 kb on - strandat 11.753 kb on - strandat 11.763 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
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9,935 - PS417_00040 0.66 -0.2
9,935 - PS417_00040 0.66 -0.1
10,043 + PS417_00040 0.70 -0.4
10,175 - PS417_00040 0.76 -0.5
10,175 - PS417_00040 0.76 -0.4
10,275 - PS417_00040 0.81 -0.9
10,275 - PS417_00040 0.81 -0.1
10,275 - PS417_00040 0.81 -0.3
10,303 + PS417_00040 0.82 -0.2
10,323 - PS417_00040 0.83 -0.1
10,328 + PS417_00040 0.83 -0.2
10,328 + PS417_00040 0.83 -0.4
10,434 + PS417_00040 0.88 +0.1
10,435 - PS417_00040 0.88 +0.3
10,435 - PS417_00040 0.88 +1.3
10,472 + PS417_00040 0.89 -0.8
10,627 + +0.4
10,741 - +0.3
10,900 - +0.2
10,900 - -0.1
11,028 + PS417_00045 0.17 -2.9
11,679 - -2.8
11,679 - -0.8
11,753 - -0.5
11,763 - +0.3

Or see this region's nucleotide sequence