Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00390 and PS417_00395 are separated by 102 nucleotides PS417_00395 and PS417_00400 are separated by 56 nucleotides PS417_00400 and PS417_00405 are separated by 74 nucleotides
PS417_00390: PS417_00390 - ABC transporter substrate-binding protein, at 75,580 to 76,500
_00390
PS417_00395: PS417_00395 - serine kinase, at 76,603 to 77,556
_00395
PS417_00400: PS417_00400 - hypothetical protein, at 77,613 to 77,903
_00400
PS417_00405: PS417_00405 - DNA polymerase I, at 77,978 to 80,755
_00405
Position (kb) 76
77
78 Strain fitness (log2 ratio) -1
0
1
2 at 75.660 kb on - strand at 75.756 kb on + strand, within PS417_00390 at 75.756 kb on + strand, within PS417_00390 at 75.757 kb on - strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.761 kb on - strand, within PS417_00390 at 76.099 kb on - strand, within PS417_00390 at 76.174 kb on + strand, within PS417_00390 at 76.259 kb on + strand, within PS417_00390 at 76.532 kb on + strand at 76.532 kb on + strand at 76.605 kb on - strand at 76.808 kb on + strand, within PS417_00395 at 77.186 kb on - strand, within PS417_00395 at 77.607 kb on - strand at 77.652 kb on - strand, within PS417_00400 at 77.897 kb on + strand at 77.897 kb on + strand at 77.897 kb on + strand at 77.898 kb on - strand at 77.898 kb on - strand at 77.898 kb on - strand at 77.906 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 75,660 - -0.5 75,756 + PS417_00390 0.19 -1.3 75,756 + PS417_00390 0.19 -0.9 75,757 - PS417_00390 0.19 -0.1 75,760 + PS417_00390 0.20 -0.1 75,760 + PS417_00390 0.20 -0.9 75,761 - PS417_00390 0.20 +0.2 76,099 - PS417_00390 0.56 -0.7 76,174 + PS417_00390 0.64 -0.9 76,259 + PS417_00390 0.74 -0.9 76,532 + -0.6 76,532 + -0.9 76,605 - +0.8 76,808 + PS417_00395 0.21 +2.7 77,186 - PS417_00395 0.61 -0.2 77,607 - -0.9 77,652 - PS417_00400 0.13 +0.5 77,897 + +0.2 77,897 + +0.1 77,897 + -0.7 77,898 - -0.9 77,898 - -0.7 77,898 - -1.3 77,906 + -0.3
Or see this region's nucleotide sequence