Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21880 and PS417_21885 are separated by 155 nucleotides PS417_21885 and PS417_21890 are separated by 389 nucleotides PS417_21890 and PS417_21895 are separated by 102 nucleotides
PS417_21880: PS417_21880 - fatty acid hydroxylase, at 4,748,756 to 4,749,619
_21880
PS417_21885: PS417_21885 - peptidase M4, at 4,749,775 to 4,750,083
_21885
PS417_21890: PS417_21890 - magnesium transporter CorA, at 4,750,473 to 4,751,495
_21890
PS417_21895: PS417_21895 - nuclease PIN, at 4,751,598 to 4,753,214
_21895
Position (kb) 4750
4751
4752 Strain fitness (log2 ratio) -1
0
1 at 4749.705 kb on + strand at 4749.706 kb on - strand at 4749.745 kb on + strand at 4749.745 kb on + strand at 4749.768 kb on - strand at 4749.980 kb on + strand, within PS417_21885 at 4750.202 kb on - strand at 4750.205 kb on + strand at 4750.206 kb on - strand at 4750.226 kb on + strand at 4750.280 kb on + strand at 4750.377 kb on + strand at 4750.814 kb on + strand, within PS417_21890 at 4750.948 kb on + strand, within PS417_21890 at 4751.032 kb on + strand, within PS417_21890 at 4751.491 kb on - strand at 4751.529 kb on + strand at 4751.529 kb on + strand at 4751.529 kb on + strand at 4751.529 kb on + strand at 4751.529 kb on + strand at 4751.530 kb on - strand at 4751.530 kb on - strand at 4751.883 kb on + strand, within PS417_21895 at 4751.883 kb on + strand, within PS417_21895 at 4751.883 kb on + strand, within PS417_21895 at 4751.883 kb on + strand, within PS417_21895 at 4751.883 kb on + strand, within PS417_21895 at 4751.884 kb on - strand, within PS417_21895 at 4751.884 kb on - strand, within PS417_21895 at 4751.884 kb on - strand, within PS417_21895 at 4752.312 kb on + strand, within PS417_21895 at 4752.313 kb on - strand, within PS417_21895 at 4752.313 kb on - strand, within PS417_21895
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 4,749,705 + +0.9 4,749,706 - +0.2 4,749,745 + -0.5 4,749,745 + +0.5 4,749,768 - -1.6 4,749,980 + PS417_21885 0.66 -1.1 4,750,202 - +0.6 4,750,205 + +0.2 4,750,206 - -0.6 4,750,226 + +0.5 4,750,280 + -0.0 4,750,377 + -0.1 4,750,814 + PS417_21890 0.33 -1.5 4,750,948 + PS417_21890 0.46 +0.1 4,751,032 + PS417_21890 0.55 -0.9 4,751,491 - -0.3 4,751,529 + -0.9 4,751,529 + -0.4 4,751,529 + -0.4 4,751,529 + -0.1 4,751,529 + +0.6 4,751,530 - -0.2 4,751,530 - -0.7 4,751,883 + PS417_21895 0.18 +0.3 4,751,883 + PS417_21895 0.18 -0.4 4,751,883 + PS417_21895 0.18 -0.6 4,751,883 + PS417_21895 0.18 -1.3 4,751,883 + PS417_21895 0.18 -0.0 4,751,884 - PS417_21895 0.18 +0.3 4,751,884 - PS417_21895 0.18 +0.3 4,751,884 - PS417_21895 0.18 +0.5 4,752,312 + PS417_21895 0.44 -1.3 4,752,313 - PS417_21895 0.44 -0.1 4,752,313 - PS417_21895 0.44 +0.1
Or see this region's nucleotide sequence