Strain Fitness in Pseudomonas simiae WCS417 around PS417_21060

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_21050 and PS417_21055 are separated by 2 nucleotidesPS417_21055 and PS417_21060 are separated by 136 nucleotidesPS417_21060 and PS417_21065 are separated by 119 nucleotides PS417_21050: PS417_21050 - serine acetyltransferase, at 4,579,972 to 4,580,964 _21050 PS417_21055: PS417_21055 - threonyl-tRNA synthetase, at 4,580,967 to 4,581,695 _21055 PS417_21060: PS417_21060 - 2-methylcitrate dehydratase, at 4,581,832 to 4,583,316 _21060 PS417_21065: PS417_21065 - 3-methylitaconate isomerase, at 4,583,436 to 4,584,626 _21065 Position (kb) 4581 4582 4583 4584Strain fitness (log2 ratio) -1 0 1at 4581.016 kb on - strandat 4581.077 kb on + strand, within PS417_21055at 4581.077 kb on + strand, within PS417_21055at 4581.077 kb on + strand, within PS417_21055at 4581.241 kb on + strand, within PS417_21055at 4581.247 kb on + strand, within PS417_21055at 4581.248 kb on - strand, within PS417_21055at 4581.801 kb on - strandat 4581.834 kb on - strandat 4582.238 kb on + strand, within PS417_21060at 4582.238 kb on + strand, within PS417_21060at 4582.238 kb on + strand, within PS417_21060at 4582.239 kb on - strand, within PS417_21060at 4582.239 kb on - strand, within PS417_21060at 4582.435 kb on + strand, within PS417_21060at 4582.452 kb on - strand, within PS417_21060at 4582.452 kb on - strand, within PS417_21060at 4582.452 kb on - strand, within PS417_21060at 4582.452 kb on - strand, within PS417_21060at 4582.452 kb on - strand, within PS417_21060at 4582.489 kb on - strand, within PS417_21060at 4582.542 kb on - strand, within PS417_21060at 4582.798 kb on + strand, within PS417_21060at 4582.798 kb on + strand, within PS417_21060at 4582.798 kb on + strand, within PS417_21060at 4582.798 kb on + strand, within PS417_21060at 4582.799 kb on - strand, within PS417_21060at 4582.799 kb on - strand, within PS417_21060at 4582.799 kb on - strand, within PS417_21060at 4582.799 kb on - strand, within PS417_21060at 4583.012 kb on + strand, within PS417_21060at 4583.237 kb on + strandat 4583.238 kb on - strandat 4583.250 kb on - strandat 4583.335 kb on - strandat 4583.479 kb on - strandat 4583.479 kb on - strandat 4583.764 kb on + strand, within PS417_21065at 4583.765 kb on - strand, within PS417_21065

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
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4,581,016 - -0.1
4,581,077 + PS417_21055 0.15 -0.9
4,581,077 + PS417_21055 0.15 +1.3
4,581,077 + PS417_21055 0.15 -0.0
4,581,241 + PS417_21055 0.38 +0.9
4,581,247 + PS417_21055 0.38 +0.1
4,581,248 - PS417_21055 0.39 -0.0
4,581,801 - +1.1
4,581,834 - -0.5
4,582,238 + PS417_21060 0.27 -0.3
4,582,238 + PS417_21060 0.27 +0.2
4,582,238 + PS417_21060 0.27 +0.2
4,582,239 - PS417_21060 0.27 -1.0
4,582,239 - PS417_21060 0.27 -0.8
4,582,435 + PS417_21060 0.41 -1.5
4,582,452 - PS417_21060 0.42 -0.6
4,582,452 - PS417_21060 0.42 +0.1
4,582,452 - PS417_21060 0.42 -0.4
4,582,452 - PS417_21060 0.42 +0.2
4,582,452 - PS417_21060 0.42 -0.3
4,582,489 - PS417_21060 0.44 -0.0
4,582,542 - PS417_21060 0.48 -0.2
4,582,798 + PS417_21060 0.65 -0.4
4,582,798 + PS417_21060 0.65 +0.0
4,582,798 + PS417_21060 0.65 -0.8
4,582,798 + PS417_21060 0.65 -0.6
4,582,799 - PS417_21060 0.65 -1.2
4,582,799 - PS417_21060 0.65 -0.9
4,582,799 - PS417_21060 0.65 -0.3
4,582,799 - PS417_21060 0.65 -1.3
4,583,012 + PS417_21060 0.79 +1.5
4,583,237 + +0.4
4,583,238 - -0.1
4,583,250 - -0.2
4,583,335 - -0.5
4,583,479 - +0.4
4,583,479 - +0.4
4,583,764 + PS417_21065 0.28 -0.1
4,583,765 - PS417_21065 0.28 +0.2

Or see this region's nucleotide sequence