Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01560 and PS417_01565 overlap by 1 nucleotides PS417_01565 and PS417_01570 are separated by 0 nucleotides PS417_01570 and PS417_01575 are separated by 39 nucleotides PS417_01575 and PS417_01580 are separated by 9 nucleotides
PS417_01560: PS417_01560 - imidazoleglycerol-phosphate dehydratase, at 346,158 to 346,751
_01560
PS417_01565: PS417_01565 - imidazole glycerol phosphate synthase, at 346,751 to 347,389
_01565
PS417_01570: PS417_01570 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, at 347,390 to 347,647
_01570
PS417_01575: PS417_01575 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) <small>(from data)</small>, at 347,687 to 348,424
_01575
PS417_01580: PS417_01580 - imidazole glycerol phosphate synthase, at 348,434 to 349,204
_01580
Position (kb) 347
348 Strain fitness (log2 ratio) -2
-1
0
1 at 346.406 kb on + strand, within PS417_01560 at 346.407 kb on - strand, within PS417_01560 at 346.446 kb on + strand, within PS417_01560 at 346.446 kb on + strand, within PS417_01560 at 346.524 kb on + strand, within PS417_01560 at 346.822 kb on - strand, within PS417_01565 at 347.216 kb on + strand, within PS417_01565 at 347.216 kb on + strand, within PS417_01565 at 347.217 kb on - strand, within PS417_01565 at 347.492 kb on - strand, within PS417_01570 at 347.558 kb on + strand, within PS417_01570 at 347.559 kb on - strand, within PS417_01570 at 347.730 kb on + strand at 347.806 kb on - strand, within PS417_01575 at 347.964 kb on - strand, within PS417_01575 at 347.985 kb on - strand, within PS417_01575 at 348.365 kb on + strand at 348.365 kb on + strand at 348.366 kb on - strand at 348.414 kb on - strand at 348.578 kb on - strand, within PS417_01580 at 348.578 kb on - strand, within PS417_01580
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 346,406 + PS417_01560 0.42 -0.6 346,407 - PS417_01560 0.42 -1.1 346,446 + PS417_01560 0.48 +1.5 346,446 + PS417_01560 0.48 -0.5 346,524 + PS417_01560 0.62 -0.0 346,822 - PS417_01565 0.11 -0.7 347,216 + PS417_01565 0.73 -0.0 347,216 + PS417_01565 0.73 -0.6 347,217 - PS417_01565 0.73 -0.8 347,492 - PS417_01570 0.40 -0.9 347,558 + PS417_01570 0.65 +0.1 347,559 - PS417_01570 0.66 -1.3 347,730 + -1.2 347,806 - PS417_01575 0.16 -0.9 347,964 - PS417_01575 0.38 -0.7 347,985 - PS417_01575 0.40 -1.6 348,365 + -0.4 348,365 + +1.3 348,366 - -2.3 348,414 - -1.7 348,578 - PS417_01580 0.19 -1.0 348,578 - PS417_01580 0.19 +0.1
Or see this region's nucleotide sequence