Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01500 and PS417_01505 are separated by 554 nucleotides PS417_01505 and PS417_01510 are separated by 108 nucleotides PS417_01510 and PS417_01515 are separated by 12 nucleotides
PS417_01500: PS417_01500 - amino acid transporter, at 332,379 to 333,152
_01500
PS417_01505: PS417_01505 - gamma-aminobutyrate transporter, at 333,707 to 335,098
_01505
PS417_01510: PS417_01510 - hypothetical protein, at 335,207 to 335,620
_01510
PS417_01515: PS417_01515 - alpha/beta hydrolase, at 335,633 to 336,577
_01515
Position (kb) 333
334
335
336 Strain fitness (log2 ratio) -1
0
1
2 at 333.077 kb on + strand at 333.087 kb on + strand at 333.103 kb on + strand at 333.104 kb on - strand at 333.218 kb on + strand at 333.218 kb on + strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.223 kb on + strand at 333.223 kb on + strand at 333.224 kb on - strand at 333.224 kb on - strand at 333.224 kb on - strand at 333.427 kb on - strand at 333.427 kb on - strand at 333.513 kb on + strand at 333.513 kb on + strand at 333.712 kb on + strand at 333.712 kb on + strand at 333.715 kb on + strand at 333.715 kb on + strand at 333.716 kb on - strand at 333.716 kb on - strand at 333.716 kb on - strand at 333.947 kb on + strand, within PS417_01505 at 333.948 kb on - strand, within PS417_01505 at 333.948 kb on - strand, within PS417_01505 at 333.948 kb on - strand, within PS417_01505 at 333.948 kb on - strand, within PS417_01505 at 333.998 kb on + strand, within PS417_01505 at 333.998 kb on + strand, within PS417_01505 at 333.999 kb on - strand, within PS417_01505 at 333.999 kb on - strand, within PS417_01505 at 334.105 kb on + strand, within PS417_01505 at 334.409 kb on - strand, within PS417_01505 at 334.451 kb on + strand, within PS417_01505 at 334.451 kb on + strand, within PS417_01505 at 334.452 kb on - strand, within PS417_01505 at 334.452 kb on - strand, within PS417_01505 at 334.516 kb on - strand, within PS417_01505 at 334.622 kb on + strand, within PS417_01505 at 334.622 kb on + strand, within PS417_01505 at 334.623 kb on - strand, within PS417_01505 at 334.709 kb on + strand, within PS417_01505 at 334.710 kb on - strand, within PS417_01505 at 334.763 kb on + strand at 334.763 kb on + strand at 334.764 kb on - strand, within PS417_01505 at 334.829 kb on + strand, within PS417_01505 at 334.928 kb on + strand, within PS417_01505 at 334.928 kb on + strand, within PS417_01505 at 334.928 kb on + strand, within PS417_01505 at 334.991 kb on - strand at 335.088 kb on + strand at 335.097 kb on - strand at 335.100 kb on + strand at 335.305 kb on + strand, within PS417_01510 at 335.306 kb on - strand, within PS417_01510 at 335.347 kb on + strand, within PS417_01510 at 335.689 kb on + strand at 335.689 kb on + strand at 335.752 kb on - strand, within PS417_01515 at 335.795 kb on + strand at 335.796 kb on - strand, within PS417_01515 at 335.796 kb on - strand, within PS417_01515 at 335.844 kb on + strand, within PS417_01515 at 335.845 kb on - strand, within PS417_01515 at 335.852 kb on + strand, within PS417_01515 at 335.870 kb on + strand, within PS417_01515 at 335.941 kb on + strand, within PS417_01515
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 333,077 + -0.5 333,087 + -0.4 333,103 + +0.1 333,104 - -0.9 333,218 + -0.2 333,218 + +1.2 333,219 - -0.0 333,219 - -0.4 333,219 - -0.6 333,219 - -0.2 333,219 - +2.1 333,219 - -1.3 333,223 + +1.8 333,223 + -0.7 333,224 - +0.5 333,224 - -0.0 333,224 - +0.4 333,427 - -0.4 333,427 - -0.2 333,513 + +0.2 333,513 + +0.6 333,712 + -0.5 333,712 + -0.3 333,715 + -0.4 333,715 + -0.9 333,716 - -0.5 333,716 - -0.8 333,716 - -0.1 333,947 + PS417_01505 0.17 -0.0 333,948 - PS417_01505 0.17 -0.6 333,948 - PS417_01505 0.17 +0.5 333,948 - PS417_01505 0.17 -1.5 333,948 - PS417_01505 0.17 -0.5 333,998 + PS417_01505 0.21 -0.3 333,998 + PS417_01505 0.21 +1.0 333,999 - PS417_01505 0.21 +0.2 333,999 - PS417_01505 0.21 -0.6 334,105 + PS417_01505 0.29 +0.3 334,409 - PS417_01505 0.50 +0.4 334,451 + PS417_01505 0.53 -0.4 334,451 + PS417_01505 0.53 +1.0 334,452 - PS417_01505 0.54 +0.0 334,452 - PS417_01505 0.54 -0.3 334,516 - PS417_01505 0.58 +0.1 334,622 + PS417_01505 0.66 -0.2 334,622 + PS417_01505 0.66 -0.1 334,623 - PS417_01505 0.66 -0.1 334,709 + PS417_01505 0.72 +0.8 334,710 - PS417_01505 0.72 -0.0 334,763 + +2.2 334,763 + +0.2 334,764 - PS417_01505 0.76 +0.3 334,829 + PS417_01505 0.81 -0.1 334,928 + PS417_01505 0.88 -0.3 334,928 + PS417_01505 0.88 +0.5 334,928 + PS417_01505 0.88 -1.1 334,991 - -0.3 335,088 + +0.2 335,097 - +0.8 335,100 + -0.4 335,305 + PS417_01510 0.24 +0.6 335,306 - PS417_01510 0.24 -0.6 335,347 + PS417_01510 0.34 -0.6 335,689 + +0.7 335,689 + +0.1 335,752 - PS417_01515 0.13 -0.5 335,795 + -0.4 335,796 - PS417_01515 0.17 -0.0 335,796 - PS417_01515 0.17 +0.4 335,844 + PS417_01515 0.22 +0.0 335,845 - PS417_01515 0.22 +0.7 335,852 + PS417_01515 0.23 +0.1 335,870 + PS417_01515 0.25 +0.3 335,941 + PS417_01515 0.33 -0.4
Or see this region's nucleotide sequence