Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01490 and PS417_01495 are separated by 74 nucleotides PS417_01495 and PS417_01500 are separated by 13 nucleotides PS417_01500 and PS417_01505 are separated by 554 nucleotides
PS417_01490: PS417_01490 - ABC transporter permease, at 330,843 to 331,538
_01490
PS417_01495: PS417_01495 - amino acid ABC transporter, at 331,613 to 332,365
_01495
PS417_01500: PS417_01500 - amino acid transporter, at 332,379 to 333,152
_01500
PS417_01505: PS417_01505 - gamma-aminobutyrate transporter, at 333,707 to 335,098
_01505
Position (kb) 332
333
334 Strain fitness (log2 ratio) -1
0
1
2 at 331.405 kb on + strand, within PS417_01490 at 331.406 kb on - strand, within PS417_01490 at 331.441 kb on - strand, within PS417_01490 at 331.635 kb on + strand at 331.635 kb on + strand at 331.635 kb on + strand at 331.636 kb on - strand at 331.636 kb on - strand at 331.656 kb on + strand at 331.656 kb on + strand at 331.657 kb on - strand at 331.752 kb on - strand, within PS417_01495 at 331.809 kb on + strand, within PS417_01495 at 331.818 kb on + strand, within PS417_01495 at 331.818 kb on + strand, within PS417_01495 at 331.818 kb on + strand, within PS417_01495 at 331.818 kb on + strand, within PS417_01495 at 331.819 kb on - strand, within PS417_01495 at 331.819 kb on - strand, within PS417_01495 at 331.819 kb on - strand, within PS417_01495 at 331.926 kb on + strand, within PS417_01495 at 331.926 kb on + strand, within PS417_01495 at 331.927 kb on - strand at 331.927 kb on - strand, within PS417_01495 at 332.041 kb on - strand, within PS417_01495 at 332.041 kb on - strand, within PS417_01495 at 332.160 kb on + strand, within PS417_01495 at 332.160 kb on + strand, within PS417_01495 at 332.160 kb on + strand, within PS417_01495 at 332.160 kb on + strand, within PS417_01495 at 332.160 kb on + strand, within PS417_01495 at 332.161 kb on - strand, within PS417_01495 at 332.161 kb on - strand, within PS417_01495 at 332.161 kb on - strand, within PS417_01495 at 332.161 kb on - strand, within PS417_01495 at 332.488 kb on + strand, within PS417_01500 at 332.573 kb on + strand, within PS417_01500 at 332.573 kb on + strand, within PS417_01500 at 332.573 kb on + strand, within PS417_01500 at 332.574 kb on - strand, within PS417_01500 at 332.574 kb on - strand, within PS417_01500 at 332.574 kb on - strand, within PS417_01500 at 332.636 kb on + strand, within PS417_01500 at 332.637 kb on - strand, within PS417_01500 at 333.077 kb on + strand at 333.087 kb on + strand at 333.103 kb on + strand at 333.104 kb on - strand at 333.218 kb on + strand at 333.218 kb on + strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.219 kb on - strand at 333.223 kb on + strand at 333.223 kb on + strand at 333.224 kb on - strand at 333.224 kb on - strand at 333.224 kb on - strand at 333.427 kb on - strand at 333.427 kb on - strand at 333.513 kb on + strand at 333.513 kb on + strand at 333.712 kb on + strand at 333.712 kb on + strand at 333.715 kb on + strand at 333.715 kb on + strand at 333.716 kb on - strand at 333.716 kb on - strand at 333.716 kb on - strand at 333.947 kb on + strand, within PS417_01505 at 333.948 kb on - strand, within PS417_01505 at 333.948 kb on - strand, within PS417_01505 at 333.948 kb on - strand, within PS417_01505 at 333.948 kb on - strand, within PS417_01505 at 333.998 kb on + strand, within PS417_01505 at 333.998 kb on + strand, within PS417_01505 at 333.999 kb on - strand, within PS417_01505 at 333.999 kb on - strand, within PS417_01505 at 334.105 kb on + strand, within PS417_01505
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 331,405 + PS417_01490 0.81 +2.2 331,406 - PS417_01490 0.81 -0.1 331,441 - PS417_01490 0.86 -0.1 331,635 + +1.5 331,635 + +0.6 331,635 + +0.0 331,636 - +0.7 331,636 - -0.0 331,656 + -0.7 331,656 + -0.3 331,657 - -1.0 331,752 - PS417_01495 0.18 -0.5 331,809 + PS417_01495 0.26 -0.4 331,818 + PS417_01495 0.27 -0.3 331,818 + PS417_01495 0.27 +0.7 331,818 + PS417_01495 0.27 +0.3 331,818 + PS417_01495 0.27 -0.5 331,819 - PS417_01495 0.27 -0.2 331,819 - PS417_01495 0.27 +0.9 331,819 - PS417_01495 0.27 -1.5 331,926 + PS417_01495 0.42 -0.3 331,926 + PS417_01495 0.42 +0.4 331,927 - +0.7 331,927 - PS417_01495 0.42 +0.2 332,041 - PS417_01495 0.57 -0.4 332,041 - PS417_01495 0.57 +0.4 332,160 + PS417_01495 0.73 +0.2 332,160 + PS417_01495 0.73 -0.2 332,160 + PS417_01495 0.73 +0.4 332,160 + PS417_01495 0.73 -0.8 332,160 + PS417_01495 0.73 +0.5 332,161 - PS417_01495 0.73 -0.1 332,161 - PS417_01495 0.73 -0.1 332,161 - PS417_01495 0.73 -1.1 332,161 - PS417_01495 0.73 -0.3 332,488 + PS417_01500 0.14 +0.0 332,573 + PS417_01500 0.25 -0.2 332,573 + PS417_01500 0.25 -0.1 332,573 + PS417_01500 0.25 -0.1 332,574 - PS417_01500 0.25 -0.1 332,574 - PS417_01500 0.25 -0.2 332,574 - PS417_01500 0.25 -0.5 332,636 + PS417_01500 0.33 -0.2 332,637 - PS417_01500 0.33 +0.3 333,077 + -0.5 333,087 + -0.4 333,103 + +0.1 333,104 - -0.9 333,218 + -0.2 333,218 + +1.2 333,219 - -0.0 333,219 - -0.4 333,219 - -0.6 333,219 - -0.2 333,219 - +2.1 333,219 - -1.3 333,223 + +1.8 333,223 + -0.7 333,224 - +0.5 333,224 - -0.0 333,224 - +0.4 333,427 - -0.4 333,427 - -0.2 333,513 + +0.2 333,513 + +0.6 333,712 + -0.5 333,712 + -0.3 333,715 + -0.4 333,715 + -0.9 333,716 - -0.5 333,716 - -0.8 333,716 - -0.1 333,947 + PS417_01505 0.17 -0.0 333,948 - PS417_01505 0.17 -0.6 333,948 - PS417_01505 0.17 +0.5 333,948 - PS417_01505 0.17 -1.5 333,948 - PS417_01505 0.17 -0.5 333,998 + PS417_01505 0.21 -0.3 333,998 + PS417_01505 0.21 +1.0 333,999 - PS417_01505 0.21 +0.2 333,999 - PS417_01505 0.21 -0.6 334,105 + PS417_01505 0.29 +0.3
Or see this region's nucleotide sequence