Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10850 and PS417_10855 overlap by 4 nucleotides PS417_10855 and PS417_10860 are separated by 64 nucleotides PS417_10860 and PS417_10865 are separated by 277 nucleotides PS417_10865 and PS417_10870 are separated by 259 nucleotides
PS417_10850: PS417_10850 - histidine kinase, at 2,319,078 to 2,320,139
_10850
PS417_10855: PS417_10855 - chemotaxis protein CheY, at 2,320,136 to 2,320,885
_10855
PS417_10860: PS417_10860 - flagellar basal body rod protein FlgG, at 2,320,950 to 2,321,735
_10860
PS417_10865: PS417_10865 - GNAT family acetyltransferase, at 2,322,013 to 2,322,468
_10865
PS417_10870: PS417_10870 - GABA permease, at 2,322,728 to 2,324,137
_10870
Position (kb)
2320
2321
2322 Strain fitness (log2 ratio)
-1
0
1 at 2320.039 kb on + strand at 2320.040 kb on - strand at 2320.048 kb on + strand at 2320.049 kb on - strand at 2320.132 kb on - strand at 2320.246 kb on + strand, within PS417_10855 at 2320.247 kb on - strand, within PS417_10855 at 2320.247 kb on - strand, within PS417_10855 at 2320.468 kb on - strand, within PS417_10855 at 2320.468 kb on - strand, within PS417_10855 at 2320.539 kb on + strand, within PS417_10855 at 2320.540 kb on - strand, within PS417_10855 at 2320.885 kb on + strand at 2320.885 kb on + strand at 2320.953 kb on + strand at 2320.953 kb on + strand at 2320.953 kb on + strand at 2320.953 kb on + strand at 2321.040 kb on - strand, within PS417_10860 at 2321.395 kb on + strand, within PS417_10860 at 2321.600 kb on - strand, within PS417_10860 at 2321.719 kb on + strand at 2321.720 kb on - strand at 2322.038 kb on + strand at 2322.038 kb on + strand at 2322.039 kb on - strand at 2322.384 kb on - strand, within PS417_10865 at 2322.512 kb on + strand at 2322.512 kb on + strand at 2322.512 kb on + strand at 2322.603 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 2,320,039 + +0.7 2,320,040 - -0.1 2,320,048 + +0.2 2,320,049 - +0.8 2,320,132 - +0.9 2,320,246 + PS417_10855 0.15 -0.3 2,320,247 - PS417_10855 0.15 +0.3 2,320,247 - PS417_10855 0.15 -0.5 2,320,468 - PS417_10855 0.44 -0.1 2,320,468 - PS417_10855 0.44 -0.3 2,320,539 + PS417_10855 0.54 -0.6 2,320,540 - PS417_10855 0.54 -0.5 2,320,885 + -0.2 2,320,885 + +1.4 2,320,953 + -0.9 2,320,953 + -0.2 2,320,953 + -0.7 2,320,953 + -0.7 2,321,040 - PS417_10860 0.11 -0.1 2,321,395 + PS417_10860 0.57 -1.2 2,321,600 - PS417_10860 0.83 -0.3 2,321,719 + -0.2 2,321,720 - -0.4 2,322,038 + +0.5 2,322,038 + +1.2 2,322,039 - -0.0 2,322,384 - PS417_10865 0.81 -0.5 2,322,512 + +0.4 2,322,512 + -0.3 2,322,512 + -0.7 2,322,603 - -0.7
Or see this region's nucleotide sequence