Strain Fitness in Pseudomonas simiae WCS417 around PS417_08125

Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_08120 and PS417_08125 overlap by 1 nucleotidesPS417_08125 and PS417_08130 are separated by 9 nucleotides PS417_08120: PS417_08120 - MFS transporter, at 1,777,978 to 1,779,063 _08120 PS417_08125: PS417_08125 - prephenate dehydratase, at 1,779,063 to 1,780,157 _08125 PS417_08130: PS417_08130 - aspartate aminotransferase, at 1,780,167 to 1,781,279 _08130 Position (kb) 1779 1780 1781Strain fitness (log2 ratio) -1 0 1 2at 1779.643 kb on - strand, within PS417_08125at 1780.163 kb on + strandat 1780.175 kb on + strandat 1780.221 kb on + strandat 1780.222 kb on - strandat 1780.764 kb on + strand, within PS417_08130at 1780.764 kb on + strand, within PS417_08130at 1780.764 kb on + strand, within PS417_08130at 1780.860 kb on + strand, within PS417_08130

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Per-strain Table

Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
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1,779,643 - PS417_08125 0.53 -0.5
1,780,163 + -0.2
1,780,175 + -0.4
1,780,221 + -1.1
1,780,222 - +2.7
1,780,764 + PS417_08130 0.54 +1.7
1,780,764 + PS417_08130 0.54 +2.7
1,780,764 + PS417_08130 0.54 +1.5
1,780,860 + PS417_08130 0.62 -1.2

Or see this region's nucleotide sequence