Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00585 and PS417_00590 are separated by 412 nucleotides PS417_00590 and PS417_00595 are separated by 284 nucleotides
PS417_00585: PS417_00585 - CoA-transferase, at 117,327 to 118,547
_00585
PS417_00590: PS417_00590 - 5S ribosomal RNA, at 118,960 to 119,075
_00590
PS417_00595: PS417_00595 - chemotaxis protein, at 119,360 to 121,240
_00595
Position (kb) 118
119
120 Strain fitness (log2 ratio) -2
-1
0
1 at 118.000 kb on - strand, within PS417_00585 at 118.000 kb on - strand, within PS417_00585 at 118.125 kb on - strand, within PS417_00585 at 118.182 kb on - strand, within PS417_00585 at 118.469 kb on + strand at 118.470 kb on - strand at 118.492 kb on + strand at 118.493 kb on - strand at 118.493 kb on - strand at 118.541 kb on + strand at 118.559 kb on + strand at 118.559 kb on + strand at 118.560 kb on - strand at 118.560 kb on - strand at 118.560 kb on - strand at 118.560 kb on - strand at 118.561 kb on + strand at 118.561 kb on + strand at 118.563 kb on + strand at 118.563 kb on + strand at 118.563 kb on + strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.564 kb on - strand at 118.613 kb on - strand at 118.662 kb on - strand at 118.662 kb on - strand at 118.972 kb on - strand, within PS417_00590 at 118.972 kb on - strand, within PS417_00590 at 119.126 kb on - strand at 119.133 kb on + strand at 119.134 kb on - strand at 119.193 kb on - strand at 119.193 kb on - strand at 119.205 kb on + strand at 119.206 kb on - strand at 119.206 kb on - strand at 119.295 kb on + strand at 119.295 kb on + strand at 119.296 kb on - strand at 119.296 kb on - strand at 119.298 kb on - strand at 119.303 kb on + strand at 119.303 kb on + strand at 119.303 kb on + strand at 119.304 kb on - strand at 119.304 kb on - strand at 119.368 kb on + strand at 119.439 kb on - strand at 119.439 kb on - strand at 119.439 kb on - strand at 119.439 kb on - strand at 119.447 kb on + strand at 119.448 kb on - strand at 119.547 kb on + strand at 119.578 kb on - strand, within PS417_00595 at 119.654 kb on + strand, within PS417_00595 at 119.654 kb on + strand, within PS417_00595 at 119.654 kb on + strand, within PS417_00595 at 119.655 kb on - strand, within PS417_00595 at 119.739 kb on - strand, within PS417_00595 at 119.739 kb on - strand, within PS417_00595 at 119.783 kb on + strand, within PS417_00595 at 119.784 kb on - strand, within PS417_00595 at 119.784 kb on - strand, within PS417_00595 at 119.784 kb on - strand, within PS417_00595 at 119.784 kb on - strand, within PS417_00595 at 119.797 kb on - strand, within PS417_00595 at 120.008 kb on + strand, within PS417_00595 at 120.008 kb on + strand, within PS417_00595 at 120.009 kb on - strand, within PS417_00595 at 120.009 kb on - strand, within PS417_00595
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 118,000 - PS417_00585 0.55 +0.2 118,000 - PS417_00585 0.55 -0.5 118,125 - PS417_00585 0.65 +0.4 118,182 - PS417_00585 0.70 +0.2 118,469 + +0.7 118,470 - +0.4 118,492 + -1.0 118,493 - +0.2 118,493 - +0.1 118,541 + -0.6 118,559 + +0.3 118,559 + -0.7 118,560 - -0.0 118,560 - -0.1 118,560 - +0.1 118,560 - -0.1 118,561 + -0.9 118,561 + -0.4 118,563 + +0.3 118,563 + -0.3 118,563 + -0.1 118,564 - -0.3 118,564 - +0.1 118,564 - -0.8 118,564 - -0.2 118,564 - +0.4 118,564 - -0.1 118,564 - -0.7 118,613 - +0.3 118,662 - -0.1 118,662 - -0.6 118,972 - PS417_00590 0.10 -0.1 118,972 - PS417_00590 0.10 +0.0 119,126 - -0.4 119,133 + +0.5 119,134 - +0.0 119,193 - +0.1 119,193 - +0.4 119,205 + -2.2 119,206 - +0.0 119,206 - +0.3 119,295 + -2.0 119,295 + +0.2 119,296 - +0.1 119,296 - +0.6 119,298 - +0.4 119,303 + -0.4 119,303 + +0.8 119,303 + -0.1 119,304 - -1.3 119,304 - +0.3 119,368 + -1.0 119,439 - -0.3 119,439 - +0.8 119,439 - -0.3 119,439 - -0.3 119,447 + +0.2 119,448 - +0.3 119,547 + -0.2 119,578 - PS417_00595 0.12 -0.1 119,654 + PS417_00595 0.16 +0.2 119,654 + PS417_00595 0.16 +0.0 119,654 + PS417_00595 0.16 +0.2 119,655 - PS417_00595 0.16 +0.2 119,739 - PS417_00595 0.20 -0.7 119,739 - PS417_00595 0.20 -0.2 119,783 + PS417_00595 0.22 -0.4 119,784 - PS417_00595 0.23 +0.0 119,784 - PS417_00595 0.23 +1.3 119,784 - PS417_00595 0.23 +0.2 119,784 - PS417_00595 0.23 -0.0 119,797 - PS417_00595 0.23 -0.0 120,008 + PS417_00595 0.34 -1.0 120,008 + PS417_00595 0.34 -0.9 120,009 - PS417_00595 0.35 -0.3 120,009 - PS417_00595 0.35 -0.7
Or see this region's nucleotide sequence