Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03880 and PS417_03885 overlap by 4 nucleotides PS417_03885 and PS417_03890 overlap by 4 nucleotides PS417_03890 and PS417_03895 overlap by 4 nucleotides PS417_03895 and PS417_03900 overlap by 4 nucleotides
PS417_03880: PS417_03880 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 870,418 to 870,633
_03880
PS417_03885: PS417_03885 - DNA gyrase inhibitor, at 870,630 to 870,830
_03885
PS417_03890: PS417_03890 - dephospho-CoA kinase, at 870,827 to 871,450
_03890
PS417_03895: PS417_03895 - methyltransferase, at 871,447 to 872,313
_03895
PS417_03900: PS417_03900 - type II secretion system protein F, at 872,310 to 873,518
_03900
Position (kb) 871
872
873 Strain fitness (log2 ratio) -2
-1
0
1 at 870.613 kb on + strand at 870.613 kb on + strand at 870.622 kb on - strand at 870.850 kb on + strand at 870.851 kb on - strand at 871.473 kb on - strand at 871.843 kb on + strand, within PS417_03895 at 871.844 kb on - strand, within PS417_03895 at 871.844 kb on - strand, within PS417_03895 at 872.052 kb on - strand, within PS417_03895 at 872.083 kb on + strand, within PS417_03895 at 872.132 kb on + strand, within PS417_03895 at 872.344 kb on + strand at 872.344 kb on + strand at 872.449 kb on + strand, within PS417_03900 at 872.450 kb on - strand, within PS417_03900 at 872.450 kb on - strand, within PS417_03900 at 872.580 kb on + strand, within PS417_03900 at 872.580 kb on + strand, within PS417_03900 at 873.010 kb on + strand, within PS417_03900 at 873.011 kb on - strand, within PS417_03900 at 873.039 kb on + strand, within PS417_03900 at 873.131 kb on - strand, within PS417_03900 at 873.131 kb on - strand, within PS417_03900 at 873.281 kb on + strand, within PS417_03900
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 870,613 + +0.2 870,613 + +0.1 870,622 - -0.1 870,850 + +0.7 870,851 - +0.9 871,473 - +0.3 871,843 + PS417_03895 0.46 -0.3 871,844 - PS417_03895 0.46 -0.6 871,844 - PS417_03895 0.46 -0.7 872,052 - PS417_03895 0.70 -0.2 872,083 + PS417_03895 0.73 -0.2 872,132 + PS417_03895 0.79 -0.4 872,344 + -0.0 872,344 + -0.2 872,449 + PS417_03900 0.11 -0.1 872,450 - PS417_03900 0.12 -0.5 872,450 - PS417_03900 0.12 -0.3 872,580 + PS417_03900 0.22 +0.4 872,580 + PS417_03900 0.22 -2.0 873,010 + PS417_03900 0.58 -0.6 873,011 - PS417_03900 0.58 -0.2 873,039 + PS417_03900 0.60 +0.7 873,131 - PS417_03900 0.68 +0.9 873,131 - PS417_03900 0.68 -0.6 873,281 + PS417_03900 0.80 +0.1
Or see this region's nucleotide sequence