Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03545 and PS417_03550 are separated by 6 nucleotides PS417_03550 and PS417_03555 are separated by 473 nucleotides PS417_03555 and PS417_03560 are separated by 392 nucleotides
PS417_03545: PS417_03545 - type III secretion protein, at 808,668 to 809,489
_03545
PS417_03550: PS417_03550 - hypothetical protein, at 809,496 to 809,858
_03550
PS417_03555: PS417_03555 - ATPase AAA, at 810,332 to 811,258
_03555
PS417_03560: PS417_03560 - GTP-binding protein, at 811,651 to 812,751
_03560
Position (kb) 810
811
812 Strain fitness (log2 ratio) -1
0
1
2 at 809.339 kb on + strand, within PS417_03545 at 809.374 kb on - strand, within PS417_03545 at 809.376 kb on + strand, within PS417_03545 at 809.401 kb on - strand, within PS417_03545 at 809.401 kb on - strand, within PS417_03545 at 809.429 kb on + strand at 809.457 kb on + strand at 809.457 kb on + strand at 809.457 kb on + strand at 809.458 kb on - strand at 809.458 kb on - strand at 809.458 kb on - strand at 809.655 kb on - strand, within PS417_03550 at 809.673 kb on + strand, within PS417_03550 at 809.717 kb on + strand, within PS417_03550 at 809.718 kb on - strand, within PS417_03550 at 809.828 kb on + strand at 809.829 kb on - strand at 810.029 kb on + strand at 810.168 kb on - strand at 810.168 kb on - strand at 810.187 kb on - strand at 810.192 kb on + strand at 810.294 kb on + strand at 810.294 kb on + strand at 810.295 kb on - strand at 810.295 kb on - strand at 810.311 kb on - strand at 810.424 kb on + strand at 810.494 kb on + strand, within PS417_03555 at 810.495 kb on - strand, within PS417_03555 at 810.542 kb on + strand, within PS417_03555 at 810.543 kb on - strand, within PS417_03555 at 810.688 kb on + strand, within PS417_03555 at 810.757 kb on - strand, within PS417_03555 at 810.905 kb on + strand, within PS417_03555 at 810.905 kb on + strand, within PS417_03555 at 810.906 kb on - strand at 810.939 kb on + strand, within PS417_03555 at 810.939 kb on + strand, within PS417_03555 at 810.939 kb on + strand, within PS417_03555 at 810.940 kb on - strand, within PS417_03555 at 810.940 kb on - strand, within PS417_03555 at 810.940 kb on - strand, within PS417_03555 at 810.940 kb on - strand, within PS417_03555 at 810.942 kb on - strand, within PS417_03555 at 810.942 kb on - strand, within PS417_03555 at 810.942 kb on - strand, within PS417_03555 at 811.099 kb on - strand, within PS417_03555 at 811.099 kb on - strand, within PS417_03555 at 811.105 kb on + strand, within PS417_03555 at 811.106 kb on - strand, within PS417_03555 at 811.120 kb on + strand, within PS417_03555 at 811.121 kb on - strand, within PS417_03555 at 811.161 kb on - strand, within PS417_03555 at 811.298 kb on - strand at 811.437 kb on + strand at 811.922 kb on + strand, within PS417_03560 at 812.135 kb on + strand, within PS417_03560 at 812.135 kb on + strand, within PS417_03560 at 812.135 kb on + strand, within PS417_03560
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 809,339 + PS417_03545 0.82 -0.1 809,374 - PS417_03545 0.86 -1.5 809,376 + PS417_03545 0.86 -0.1 809,401 - PS417_03545 0.89 +0.9 809,401 - PS417_03545 0.89 -0.3 809,429 + +0.4 809,457 + -0.3 809,457 + -0.1 809,457 + +0.1 809,458 - -0.6 809,458 - +0.3 809,458 - -0.3 809,655 - PS417_03550 0.44 +0.8 809,673 + PS417_03550 0.49 -0.3 809,717 + PS417_03550 0.61 +0.4 809,718 - PS417_03550 0.61 -0.3 809,828 + +0.1 809,829 - -0.4 810,029 + +0.3 810,168 - -1.6 810,168 - +0.4 810,187 - +0.3 810,192 + -0.2 810,294 + -0.0 810,294 + +0.1 810,295 - +0.6 810,295 - -0.1 810,311 - -0.2 810,424 + +0.1 810,494 + PS417_03555 0.17 +0.3 810,495 - PS417_03555 0.18 -0.1 810,542 + PS417_03555 0.23 -0.5 810,543 - PS417_03555 0.23 +0.4 810,688 + PS417_03555 0.38 -0.4 810,757 - PS417_03555 0.46 -0.5 810,905 + PS417_03555 0.62 -0.2 810,905 + PS417_03555 0.62 +0.4 810,906 - -0.5 810,939 + PS417_03555 0.65 +0.1 810,939 + PS417_03555 0.65 +0.0 810,939 + PS417_03555 0.65 -0.0 810,940 - PS417_03555 0.66 -0.5 810,940 - PS417_03555 0.66 +0.3 810,940 - PS417_03555 0.66 -0.2 810,940 - PS417_03555 0.66 -0.4 810,942 - PS417_03555 0.66 -0.1 810,942 - PS417_03555 0.66 -0.1 810,942 - PS417_03555 0.66 -0.6 811,099 - PS417_03555 0.83 +0.2 811,099 - PS417_03555 0.83 -0.0 811,105 + PS417_03555 0.83 -0.3 811,106 - PS417_03555 0.83 -0.9 811,120 + PS417_03555 0.85 -1.2 811,121 - PS417_03555 0.85 +0.1 811,161 - PS417_03555 0.89 -0.3 811,298 - -0.6 811,437 + +1.3 811,922 + PS417_03560 0.25 +2.0 812,135 + PS417_03560 0.44 -1.7 812,135 + PS417_03560 0.44 -0.3 812,135 + PS417_03560 0.44 -1.5
Or see this region's nucleotide sequence