Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_27060 and PS417_27065 are separated by 131 nucleotides PS417_27065 and PS417_27070 are separated by 20 nucleotides PS417_27070 and PS417_27075 are separated by 99 nucleotides PS417_27075 and PS417_27080 are separated by 30 nucleotides
PS417_27060: PS417_27060 - 2OG-Fe(II) oxygenase, at 5,842,187 to 5,842,819
_27060
PS417_27065: PS417_27065 - hypothetical protein, at 5,842,951 to 5,843,703
_27065
PS417_27070: PS417_27070 - alpha/beta hydrolase, at 5,843,724 to 5,844,650
_27070
PS417_27075: PS417_27075 - thiol-disulfide isomerase, at 5,844,750 to 5,845,325
_27075
PS417_27080: PS417_27080 - penicillin amidase, at 5,845,356 to 5,847,710
_27080
Position (kb)
5843
5844
5845 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5842.774 kb on + strand at 5842.774 kb on + strand at 5842.774 kb on + strand at 5842.774 kb on + strand at 5842.775 kb on - strand at 5842.775 kb on - strand at 5842.775 kb on - strand at 5842.802 kb on + strand at 5842.803 kb on - strand at 5842.819 kb on + strand at 5842.819 kb on + strand at 5842.820 kb on - strand at 5842.822 kb on + strand at 5842.823 kb on - strand at 5842.823 kb on - strand at 5842.823 kb on - strand at 5842.828 kb on + strand at 5842.866 kb on + strand at 5842.874 kb on - strand at 5842.874 kb on - strand at 5842.927 kb on - strand at 5843.023 kb on + strand at 5843.024 kb on - strand at 5843.024 kb on - strand at 5843.120 kb on - strand, within PS417_27065 at 5843.120 kb on - strand, within PS417_27065 at 5843.554 kb on + strand, within PS417_27065 at 5843.555 kb on - strand, within PS417_27065 at 5843.555 kb on - strand, within PS417_27065 at 5843.630 kb on - strand at 5843.632 kb on + strand at 5843.633 kb on - strand at 5843.633 kb on - strand at 5843.633 kb on - strand at 5843.718 kb on + strand at 5843.719 kb on - strand at 5843.725 kb on + strand at 5843.725 kb on + strand at 5843.725 kb on + strand at 5843.726 kb on - strand at 5843.726 kb on - strand at 5843.753 kb on + strand at 5843.753 kb on + strand at 5843.753 kb on + strand at 5843.753 kb on + strand at 5843.753 kb on + strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.754 kb on - strand at 5843.759 kb on - strand at 5843.759 kb on - strand at 5844.091 kb on + strand, within PS417_27070 at 5844.091 kb on + strand, within PS417_27070 at 5844.092 kb on - strand, within PS417_27070 at 5844.094 kb on + strand, within PS417_27070 at 5844.094 kb on + strand, within PS417_27070 at 5844.094 kb on + strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.095 kb on - strand, within PS417_27070 at 5844.298 kb on + strand, within PS417_27070 at 5844.431 kb on - strand, within PS417_27070 at 5844.650 kb on + strand at 5844.651 kb on - strand at 5844.724 kb on + strand at 5844.725 kb on - strand at 5844.725 kb on - strand at 5844.725 kb on - strand at 5844.741 kb on + strand at 5844.742 kb on - strand at 5844.742 kb on - strand at 5844.826 kb on - strand, within PS417_27075 at 5844.826 kb on - strand, within PS417_27075 at 5845.035 kb on + strand, within PS417_27075 at 5845.035 kb on + strand, within PS417_27075 at 5845.188 kb on + strand, within PS417_27075 at 5845.208 kb on + strand, within PS417_27075 at 5845.209 kb on - strand, within PS417_27075 at 5845.226 kb on + strand, within PS417_27075 at 5845.267 kb on + strand, within PS417_27075 at 5845.268 kb on - strand at 5845.419 kb on + strand at 5845.419 kb on + strand at 5845.419 kb on + strand at 5845.420 kb on - strand at 5845.425 kb on + strand at 5845.425 kb on + strand at 5845.560 kb on + strand at 5845.560 kb on + strand at 5845.560 kb on + strand at 5845.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 5,842,774 + -0.1 5,842,774 + -0.1 5,842,774 + +0.3 5,842,774 + +0.7 5,842,775 - -0.1 5,842,775 - -0.0 5,842,775 - -0.8 5,842,802 + -0.2 5,842,803 - -1.5 5,842,819 + +1.2 5,842,819 + +1.0 5,842,820 - -0.4 5,842,822 + -0.3 5,842,823 - -0.6 5,842,823 - +0.1 5,842,823 - -0.3 5,842,828 + -0.5 5,842,866 + -0.1 5,842,874 - -0.1 5,842,874 - +0.5 5,842,927 - +0.5 5,843,023 + -0.0 5,843,024 - -0.1 5,843,024 - +1.1 5,843,120 - PS417_27065 0.22 +0.4 5,843,120 - PS417_27065 0.22 +0.4 5,843,554 + PS417_27065 0.80 +0.0 5,843,555 - PS417_27065 0.80 +1.3 5,843,555 - PS417_27065 0.80 -0.3 5,843,630 - +0.1 5,843,632 + -0.7 5,843,633 - -0.6 5,843,633 - +0.2 5,843,633 - +0.5 5,843,718 + -0.3 5,843,719 - +0.3 5,843,725 + +0.4 5,843,725 + +0.1 5,843,725 + +0.2 5,843,726 - +0.2 5,843,726 - +0.0 5,843,753 + -0.6 5,843,753 + -0.3 5,843,753 + -0.5 5,843,753 + -1.1 5,843,753 + -1.3 5,843,754 - +0.9 5,843,754 - -0.2 5,843,754 - -0.2 5,843,754 - -0.1 5,843,754 - +0.1 5,843,754 - -0.2 5,843,759 - -0.4 5,843,759 - -0.0 5,844,091 + PS417_27070 0.40 -0.4 5,844,091 + PS417_27070 0.40 +0.0 5,844,092 - PS417_27070 0.40 -0.8 5,844,094 + PS417_27070 0.40 -0.3 5,844,094 + PS417_27070 0.40 -0.2 5,844,094 + PS417_27070 0.40 -1.0 5,844,095 - PS417_27070 0.40 -0.4 5,844,095 - PS417_27070 0.40 -0.0 5,844,095 - PS417_27070 0.40 -0.5 5,844,095 - PS417_27070 0.40 -0.2 5,844,095 - PS417_27070 0.40 -0.0 5,844,298 + PS417_27070 0.62 -0.4 5,844,431 - PS417_27070 0.76 -2.1 5,844,650 + +0.6 5,844,651 - -0.2 5,844,724 + +0.1 5,844,725 - +0.2 5,844,725 - -0.2 5,844,725 - -0.1 5,844,741 + -0.4 5,844,742 - +0.1 5,844,742 - +0.7 5,844,826 - PS417_27075 0.13 +0.2 5,844,826 - PS417_27075 0.13 -0.6 5,845,035 + PS417_27075 0.49 -0.3 5,845,035 + PS417_27075 0.49 +3.1 5,845,188 + PS417_27075 0.76 +0.2 5,845,208 + PS417_27075 0.80 +2.1 5,845,209 - PS417_27075 0.80 +1.1 5,845,226 + PS417_27075 0.83 +0.5 5,845,267 + PS417_27075 0.90 +2.1 5,845,268 - -0.3 5,845,419 + -2.1 5,845,419 + -0.1 5,845,419 + -0.2 5,845,420 - -0.1 5,845,425 + -0.1 5,845,425 + -0.1 5,845,560 + +0.5 5,845,560 + +1.3 5,845,560 + +0.6 5,845,561 - -0.0
Or see this region's nucleotide sequence