Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24830 and PS417_24835 are separated by 305 nucleotides PS417_24835 and PS417_24840 are separated by 6 nucleotides PS417_24840 and PS417_24845 are separated by 109 nucleotides PS417_24845 and PS417_24850 are separated by 214 nucleotides
PS417_24830: PS417_24830 - GNAT family acetyltransferase, at 5,386,297 to 5,386,755
_24830
PS417_24835: PS417_24835 - hypothetical protein, at 5,387,061 to 5,387,717
_24835
PS417_24840: PS417_24840 - hypothetical protein, at 5,387,724 to 5,388,539
_24840
PS417_24845: PS417_24845 - inorganic pyrophosphatase, at 5,388,649 to 5,389,176
_24845
PS417_24850: PS417_24850 - transcriptional regulator, at 5,389,391 to 5,390,167
_24850
Position (kb)
5387
5388
5389 Strain fitness (log2 ratio)
-2
-1
0
1 at 5386.729 kb on + strand at 5386.730 kb on - strand at 5386.887 kb on + strand at 5386.887 kb on + strand at 5386.887 kb on + strand at 5386.888 kb on - strand at 5386.954 kb on - strand at 5386.975 kb on + strand at 5386.990 kb on - strand at 5386.990 kb on - strand at 5386.990 kb on - strand at 5386.990 kb on - strand at 5386.998 kb on - strand at 5387.103 kb on + strand at 5387.120 kb on - strand at 5387.127 kb on + strand, within PS417_24835 at 5387.143 kb on - strand at 5387.316 kb on + strand, within PS417_24835 at 5387.316 kb on + strand, within PS417_24835 at 5387.316 kb on + strand, within PS417_24835 at 5387.317 kb on - strand, within PS417_24835 at 5387.317 kb on - strand, within PS417_24835 at 5387.317 kb on - strand, within PS417_24835 at 5388.168 kb on + strand, within PS417_24840 at 5388.291 kb on + strand, within PS417_24840 at 5388.292 kb on - strand, within PS417_24840 at 5388.466 kb on - strand at 5388.544 kb on - strand at 5388.582 kb on + strand at 5389.310 kb on + strand at 5389.316 kb on + strand at 5389.317 kb on - strand at 5389.317 kb on - strand at 5389.317 kb on - strand at 5389.328 kb on + strand at 5389.329 kb on - strand at 5389.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 5,386,729 + +0.3 5,386,730 - +0.5 5,386,887 + +0.1 5,386,887 + +0.6 5,386,887 + +0.7 5,386,888 - +0.1 5,386,954 - +0.1 5,386,975 + +0.3 5,386,990 - +0.1 5,386,990 - -0.1 5,386,990 - -0.3 5,386,990 - +0.3 5,386,998 - -0.2 5,387,103 + +0.3 5,387,120 - -0.7 5,387,127 + PS417_24835 0.10 -0.4 5,387,143 - -0.1 5,387,316 + PS417_24835 0.39 -0.7 5,387,316 + PS417_24835 0.39 -0.5 5,387,316 + PS417_24835 0.39 -0.7 5,387,317 - PS417_24835 0.39 -0.8 5,387,317 - PS417_24835 0.39 -0.9 5,387,317 - PS417_24835 0.39 -0.4 5,388,168 + PS417_24840 0.54 -0.4 5,388,291 + PS417_24840 0.69 -0.9 5,388,292 - PS417_24840 0.70 -0.1 5,388,466 - -0.2 5,388,544 - -0.0 5,388,582 + -0.2 5,389,310 + -0.5 5,389,316 + +0.5 5,389,317 - -0.7 5,389,317 - +0.4 5,389,317 - -0.0 5,389,328 + -1.8 5,389,329 - +0.0 5,389,397 - -0.3
Or see this region's nucleotide sequence