Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22775 and PS417_22780 overlap by 11 nucleotides PS417_22780 and PS417_22785 are separated by 367 nucleotides
PS417_22775: PS417_22775 - histidine kinase, at 4,935,084 to 4,936,364
_22775
PS417_22780: PS417_22780 - XRE family transcriptional regulator, at 4,936,354 to 4,937,037
_22780
PS417_22785: PS417_22785 - molecular chaperone GroEL, at 4,937,405 to 4,939,051
_22785
Position (kb)
4936
4937
4938 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4935.395 kb on + strand, within PS417_22775 at 4935.401 kb on + strand, within PS417_22775 at 4935.403 kb on + strand, within PS417_22775 at 4935.404 kb on - strand, within PS417_22775 at 4935.433 kb on + strand, within PS417_22775 at 4935.434 kb on - strand, within PS417_22775 at 4935.434 kb on - strand, within PS417_22775 at 4935.434 kb on - strand, within PS417_22775 at 4935.921 kb on + strand, within PS417_22775 at 4936.019 kb on - strand, within PS417_22775 at 4936.055 kb on - strand, within PS417_22775 at 4936.104 kb on + strand, within PS417_22775 at 4936.105 kb on - strand, within PS417_22775 at 4936.147 kb on + strand, within PS417_22775 at 4936.147 kb on + strand at 4936.635 kb on - strand, within PS417_22780 at 4936.742 kb on + strand, within PS417_22780 at 4936.905 kb on - strand, within PS417_22780 at 4936.962 kb on + strand, within PS417_22780 at 4936.962 kb on + strand, within PS417_22780 at 4936.963 kb on - strand, within PS417_22780 at 4936.964 kb on + strand at 4936.982 kb on + strand at 4936.983 kb on - strand at 4937.004 kb on + strand at 4937.086 kb on - strand at 4937.132 kb on - strand at 4937.150 kb on - strand at 4937.190 kb on + strand at 4937.190 kb on + strand at 4937.191 kb on - strand at 4937.300 kb on + strand at 4937.301 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 4,935,395 + PS417_22775 0.24 +0.1 4,935,401 + PS417_22775 0.25 -0.2 4,935,403 + PS417_22775 0.25 -0.6 4,935,404 - PS417_22775 0.25 -0.2 4,935,433 + PS417_22775 0.27 -0.4 4,935,434 - PS417_22775 0.27 +0.5 4,935,434 - PS417_22775 0.27 -0.2 4,935,434 - PS417_22775 0.27 +0.5 4,935,921 + PS417_22775 0.65 +0.1 4,936,019 - PS417_22775 0.73 +0.1 4,936,055 - PS417_22775 0.76 +0.7 4,936,104 + PS417_22775 0.80 +0.0 4,936,105 - PS417_22775 0.80 -0.1 4,936,147 + PS417_22775 0.83 -0.5 4,936,147 + -1.8 4,936,635 - PS417_22780 0.41 -0.2 4,936,742 + PS417_22780 0.57 -0.5 4,936,905 - PS417_22780 0.81 -0.3 4,936,962 + PS417_22780 0.89 -0.8 4,936,962 + PS417_22780 0.89 -0.2 4,936,963 - PS417_22780 0.89 -0.1 4,936,964 + +0.3 4,936,982 + -0.1 4,936,983 - +2.0 4,937,004 + +0.3 4,937,086 - -0.1 4,937,132 - +0.2 4,937,150 - -0.8 4,937,190 + +0.7 4,937,190 + +0.1 4,937,191 - +0.6 4,937,300 + +1.0 4,937,301 - +0.1
Or see this region's nucleotide sequence