Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22340 and PS417_22345 are separated by 18 nucleotides PS417_22345 and PS417_22350 are separated by 173 nucleotides PS417_22350 and PS417_22355 are separated by 405 nucleotides PS417_22355 and PS417_22360 are separated by 10 nucleotides
PS417_22340: PS417_22340 - carbamate kinase, at 4,839,738 to 4,840,667
_22340
PS417_22345: PS417_22345 - acetyl-CoA carboxylase, at 4,840,686 to 4,841,048
_22345
PS417_22350: PS417_22350 - Fis family transcriptional regulator, at 4,841,222 to 4,842,730
_22350
PS417_22355: PS417_22355 - glycine cleavage system protein H, at 4,843,136 to 4,843,519
_22355
PS417_22360: PS417_22360 - Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data) , at 4,843,530 to 4,846,370
_22360
Position (kb)
4841
4842
4843 Strain fitness (log2 ratio)
-1
0
1 at 4840.308 kb on + strand, within PS417_22340 at 4840.308 kb on + strand, within PS417_22340 at 4840.309 kb on - strand, within PS417_22340 at 4840.434 kb on - strand, within PS417_22340 at 4840.659 kb on + strand at 4840.660 kb on - strand at 4840.660 kb on - strand at 4840.670 kb on + strand at 4840.671 kb on - strand at 4840.685 kb on + strand at 4840.686 kb on - strand at 4840.747 kb on - strand, within PS417_22345 at 4840.752 kb on + strand, within PS417_22345 at 4840.752 kb on + strand, within PS417_22345 at 4840.752 kb on + strand, within PS417_22345 at 4840.753 kb on - strand, within PS417_22345 at 4840.753 kb on - strand, within PS417_22345 at 4840.753 kb on - strand, within PS417_22345 at 4841.008 kb on + strand, within PS417_22345 at 4841.008 kb on + strand, within PS417_22345 at 4841.047 kb on - strand at 4841.111 kb on + strand at 4841.111 kb on + strand at 4841.112 kb on - strand at 4841.113 kb on + strand at 4841.114 kb on - strand at 4841.114 kb on - strand at 4841.183 kb on - strand at 4841.183 kb on - strand at 4841.337 kb on - strand at 4841.337 kb on - strand at 4841.556 kb on - strand, within PS417_22350 at 4842.292 kb on - strand, within PS417_22350 at 4842.626 kb on + strand at 4842.626 kb on + strand at 4842.881 kb on - strand at 4843.015 kb on + strand at 4843.311 kb on + strand, within PS417_22355 at 4843.312 kb on - strand, within PS417_22355 at 4843.331 kb on + strand, within PS417_22355 at 4843.332 kb on - strand, within PS417_22355 at 4843.332 kb on - strand, within PS417_22355 at 4843.436 kb on - strand, within PS417_22355 at 4843.485 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 4,840,308 + PS417_22340 0.61 -0.3 4,840,308 + PS417_22340 0.61 -0.3 4,840,309 - PS417_22340 0.61 -0.0 4,840,434 - PS417_22340 0.75 +0.3 4,840,659 + +0.1 4,840,660 - +0.1 4,840,660 - -0.7 4,840,670 + +0.3 4,840,671 - -0.0 4,840,685 + +0.0 4,840,686 - +0.2 4,840,747 - PS417_22345 0.17 -0.1 4,840,752 + PS417_22345 0.18 +0.0 4,840,752 + PS417_22345 0.18 -0.0 4,840,752 + PS417_22345 0.18 +0.7 4,840,753 - PS417_22345 0.18 -0.1 4,840,753 - PS417_22345 0.18 +0.9 4,840,753 - PS417_22345 0.18 -0.8 4,841,008 + PS417_22345 0.89 -0.0 4,841,008 + PS417_22345 0.89 -0.6 4,841,047 - -0.4 4,841,111 + +0.0 4,841,111 + +0.2 4,841,112 - +0.0 4,841,113 + -0.0 4,841,114 - -0.1 4,841,114 - +0.2 4,841,183 - +0.4 4,841,183 - +0.6 4,841,337 - -0.6 4,841,337 - -1.1 4,841,556 - PS417_22350 0.22 -0.4 4,842,292 - PS417_22350 0.71 -0.5 4,842,626 + +0.6 4,842,626 + +0.3 4,842,881 - -0.1 4,843,015 + -0.0 4,843,311 + PS417_22355 0.46 +0.1 4,843,312 - PS417_22355 0.46 -0.7 4,843,331 + PS417_22355 0.51 -0.2 4,843,332 - PS417_22355 0.51 -0.2 4,843,332 - PS417_22355 0.51 -0.1 4,843,436 - PS417_22355 0.78 -0.3 4,843,485 - -0.2
Or see this region's nucleotide sequence