Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_20785 and PS417_20790 are separated by 80 nucleotides PS417_20790 and PS417_20795 are separated by 134 nucleotides PS417_20795 and PS417_20800 are separated by 132 nucleotides
PS417_20785: PS417_20785 - transcriptional regulator, at 4,515,588 to 4,516,322
_20785
PS417_20790: PS417_20790 - adenine phosphoribosyltransferase, at 4,516,403 to 4,516,951
_20790
PS417_20795: PS417_20795 - acyl-CoA dehydrogenase, at 4,517,086 to 4,518,234
_20795
PS417_20800: PS417_20800 - recombination protein RecR, at 4,518,367 to 4,518,969
_20800
Position (kb) 4517
4518
4519 Strain fitness (log2 ratio) -2
-1
0
1 at 4516.180 kb on - strand, within PS417_20785 at 4516.269 kb on - strand at 4516.363 kb on + strand at 4516.366 kb on - strand at 4516.486 kb on + strand, within PS417_20790 at 4516.487 kb on - strand, within PS417_20790 at 4516.487 kb on - strand, within PS417_20790 at 4516.487 kb on - strand, within PS417_20790 at 4516.547 kb on + strand, within PS417_20790 at 4516.547 kb on + strand, within PS417_20790 at 4516.548 kb on - strand, within PS417_20790 at 4516.619 kb on + strand, within PS417_20790 at 4516.620 kb on - strand, within PS417_20790 at 4517.018 kb on + strand at 4517.018 kb on + strand at 4517.018 kb on + strand at 4517.018 kb on + strand at 4517.155 kb on - strand at 4517.168 kb on + strand at 4517.198 kb on + strand at 4517.198 kb on + strand at 4517.199 kb on - strand at 4517.199 kb on - strand at 4517.231 kb on + strand, within PS417_20795 at 4517.231 kb on + strand, within PS417_20795 at 4517.232 kb on - strand, within PS417_20795 at 4517.647 kb on - strand, within PS417_20795 at 4517.726 kb on + strand, within PS417_20795 at 4518.101 kb on + strand, within PS417_20795 at 4518.215 kb on + strand at 4518.215 kb on + strand at 4518.216 kb on - strand at 4518.369 kb on - strand at 4518.369 kb on - strand at 4518.506 kb on + strand, within PS417_20800 at 4518.506 kb on + strand, within PS417_20800 at 4518.506 kb on + strand, within PS417_20800 at 4518.506 kb on + strand, within PS417_20800 at 4518.506 kb on + strand, within PS417_20800 at 4518.506 kb on + strand, within PS417_20800 at 4518.507 kb on - strand, within PS417_20800 at 4518.507 kb on - strand, within PS417_20800 at 4518.507 kb on - strand, within PS417_20800 at 4518.507 kb on - strand, within PS417_20800 at 4518.562 kb on - strand, within PS417_20800 at 4518.662 kb on + strand, within PS417_20800 at 4518.695 kb on + strand, within PS417_20800 at 4518.696 kb on - strand, within PS417_20800 at 4519.070 kb on + strand at 4519.071 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 4,516,180 - PS417_20785 0.81 +0.9 4,516,269 - +0.9 4,516,363 + -0.8 4,516,366 - +0.1 4,516,486 + PS417_20790 0.15 +0.1 4,516,487 - PS417_20790 0.15 +0.7 4,516,487 - PS417_20790 0.15 +0.5 4,516,487 - PS417_20790 0.15 -0.1 4,516,547 + PS417_20790 0.26 +0.1 4,516,547 + PS417_20790 0.26 -0.1 4,516,548 - PS417_20790 0.26 +0.1 4,516,619 + PS417_20790 0.39 +0.3 4,516,620 - PS417_20790 0.40 -0.5 4,517,018 + -0.9 4,517,018 + +1.0 4,517,018 + -0.1 4,517,018 + -0.5 4,517,155 - +0.6 4,517,168 + -0.0 4,517,198 + -2.3 4,517,198 + +0.9 4,517,199 - -0.0 4,517,199 - -0.2 4,517,231 + PS417_20795 0.13 -0.8 4,517,231 + PS417_20795 0.13 +0.1 4,517,232 - PS417_20795 0.13 -0.5 4,517,647 - PS417_20795 0.49 +0.1 4,517,726 + PS417_20795 0.56 -0.3 4,518,101 + PS417_20795 0.88 -1.4 4,518,215 + -0.2 4,518,215 + +0.0 4,518,216 - -0.4 4,518,369 - +0.6 4,518,369 - -0.4 4,518,506 + PS417_20800 0.23 +0.0 4,518,506 + PS417_20800 0.23 +0.2 4,518,506 + PS417_20800 0.23 -1.1 4,518,506 + PS417_20800 0.23 +0.6 4,518,506 + PS417_20800 0.23 +0.2 4,518,506 + PS417_20800 0.23 +0.5 4,518,507 - PS417_20800 0.23 +0.4 4,518,507 - PS417_20800 0.23 +1.1 4,518,507 - PS417_20800 0.23 -0.4 4,518,507 - PS417_20800 0.23 +0.4 4,518,562 - PS417_20800 0.32 -0.2 4,518,662 + PS417_20800 0.49 -0.1 4,518,695 + PS417_20800 0.54 -0.0 4,518,696 - PS417_20800 0.55 -0.1 4,519,070 + +0.1 4,519,071 - +0.0
Or see this region's nucleotide sequence