Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11255 and PS417_11260 overlap by 4 nucleotides PS417_11260 and PS417_11265 are separated by 93 nucleotides PS417_11265 and PS417_11270 overlap by 14 nucleotides PS417_11270 and PS417_11275 are separated by 148 nucleotides PS417_11275 and PS417_11280 are separated by 8 nucleotides
PS417_11255: PS417_11255 - general secretion pathway protein GspJ, at 2,411,841 to 2,412,452
_11255
PS417_11260: PS417_11260 - general secretion pathway protein GspH, at 2,412,449 to 2,412,901
_11260
PS417_11265: PS417_11265 - general secretion pathway protein GspC, at 2,412,995 to 2,413,426
_11265
PS417_11270: PS417_11270 - general secretion pathway protein GspI, at 2,413,413 to 2,413,778
_11270
PS417_11275: PS417_11275 - general secretion pathway protein GspG, at 2,413,927 to 2,414,376
_11275
PS417_11280: PS417_11280 - HxcX atypical pseudopilin, at 2,414,385 to 2,415,254
_11280
Position (kb)
2412
2413
2414 Strain fitness (log2 ratio)
-1
0
1 at 2412.420 kb on + strand at 2412.421 kb on - strand at 2412.861 kb on - strand at 2413.748 kb on + strand at 2413.774 kb on + strand at 2413.775 kb on - strand at 2413.863 kb on + strand at 2414.129 kb on - strand, within PS417_11275 at 2414.269 kb on + strand, within PS417_11275 at 2414.269 kb on + strand at 2414.345 kb on + strand at 2414.345 kb on + strand at 2414.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 2,412,420 + +0.0 2,412,421 - +0.2 2,412,861 - +0.9 2,413,748 + -0.2 2,413,774 + +0.5 2,413,775 - +0.2 2,413,863 + -0.5 2,414,129 - PS417_11275 0.45 +1.6 2,414,269 + PS417_11275 0.76 -0.1 2,414,269 + +0.9 2,414,345 + -0.3 2,414,345 + -0.1 2,414,375 - -0.2
Or see this region's nucleotide sequence