Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01020 and PS417_01025 overlap by 4 nucleotides PS417_01025 and PS417_01030 overlap by 4 nucleotides PS417_01030 and PS417_01035 are separated by 99 nucleotides
PS417_01020: PS417_01020 - glutamine ABC transporter permease, at 222,515 to 223,174
_01020
PS417_01025: PS417_01025 - glutamine ABC transporter ATP-binding protein, at 223,171 to 223,947
_01025
PS417_01030: PS417_01030 - glutamine ABC transporter substrate-binding protein, at 223,944 to 224,798
_01030
PS417_01035: PS417_01035 - ATP-binding protein, at 224,898 to 226,058
_01035
Position (kb) 223
224
225 Strain fitness (log2 ratio) -2
-1
0
1 at 223.067 kb on + strand, within PS417_01020 at 223.067 kb on + strand, within PS417_01020 at 223.068 kb on - strand, within PS417_01020 at 223.068 kb on - strand, within PS417_01020 at 223.086 kb on - strand, within PS417_01020 at 223.114 kb on + strand at 223.115 kb on - strand at 223.435 kb on + strand, within PS417_01025 at 223.944 kb on - strand at 224.134 kb on - strand, within PS417_01030 at 224.433 kb on - strand, within PS417_01030 at 224.724 kb on + strand at 224.724 kb on + strand at 224.725 kb on - strand at 224.725 kb on - strand at 224.838 kb on + strand at 224.838 kb on + strand at 224.838 kb on + strand at 224.839 kb on - strand at 224.839 kb on - strand at 224.887 kb on - strand at 224.899 kb on + strand at 224.900 kb on - strand at 224.988 kb on - strand at 225.184 kb on + strand, within PS417_01035 at 225.553 kb on + strand, within PS417_01035 at 225.553 kb on + strand, within PS417_01035 at 225.554 kb on - strand, within PS417_01035 at 225.554 kb on - strand, within PS417_01035
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 2 remove 223,067 + PS417_01020 0.84 -0.6 223,067 + PS417_01020 0.84 +0.0 223,068 - PS417_01020 0.84 -0.2 223,068 - PS417_01020 0.84 +0.8 223,086 - PS417_01020 0.87 +0.4 223,114 + -0.2 223,115 - -0.1 223,435 + PS417_01025 0.34 +0.7 223,944 - -0.1 224,134 - PS417_01030 0.22 -1.8 224,433 - PS417_01030 0.57 -0.4 224,724 + -0.1 224,724 + -0.2 224,725 - -0.3 224,725 - +1.0 224,838 + +0.8 224,838 + -0.1 224,838 + +0.3 224,839 - +0.1 224,839 - +0.7 224,887 - -1.1 224,899 + -0.5 224,900 - -0.2 224,988 - -0.2 225,184 + PS417_01035 0.25 -0.5 225,553 + PS417_01035 0.56 -0.6 225,553 + PS417_01035 0.56 -0.0 225,554 - PS417_01035 0.57 -0.0 225,554 - PS417_01035 0.57 -0.1
Or see this region's nucleotide sequence