Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00435 and PS417_00440 are separated by 88 nucleotides PS417_00440 and PS417_00445 overlap by 4 nucleotides PS417_00445 and PS417_00450 are separated by 10 nucleotides
PS417_00435: PS417_00435 - DeoR faimly transcriptional regulator, at 84,512 to 86,566
_00435
PS417_00440: PS417_00440 - N-acetylmuramoyl-L-alanine amidase, at 86,655 to 87,431
_00440
PS417_00445: PS417_00445 - histidine kinase, at 87,428 to 89,215
_00445
PS417_00450: PS417_00450 - chemotaxis protein CheY, at 89,226 to 90,572
_00450
Position (kb) 87
88
89
90 Strain fitness (log2 ratio) -2
-1
0
1
2 at 86.522 kb on + strand at 86.522 kb on + strand at 86.523 kb on - strand at 86.523 kb on - strand at 86.564 kb on + strand at 86.641 kb on + strand at 86.642 kb on - strand at 86.642 kb on - strand at 86.642 kb on - strand at 86.667 kb on + strand at 86.667 kb on + strand at 86.874 kb on + strand, within PS417_00440 at 86.874 kb on + strand, within PS417_00440 at 86.874 kb on + strand, within PS417_00440 at 86.874 kb on + strand, within PS417_00440 at 86.875 kb on - strand, within PS417_00440 at 86.875 kb on - strand, within PS417_00440 at 87.016 kb on - strand, within PS417_00440 at 87.039 kb on - strand, within PS417_00440 at 87.039 kb on - strand, within PS417_00440 at 87.190 kb on + strand, within PS417_00440 at 87.231 kb on + strand, within PS417_00440 at 87.231 kb on + strand, within PS417_00440 at 87.232 kb on - strand, within PS417_00440 at 87.232 kb on - strand, within PS417_00440 at 87.447 kb on + strand at 87.447 kb on + strand at 87.447 kb on + strand at 87.448 kb on - strand at 87.448 kb on - strand at 87.448 kb on - strand at 87.448 kb on - strand at 87.478 kb on - strand at 87.600 kb on + strand at 87.753 kb on + strand, within PS417_00445 at 87.754 kb on - strand, within PS417_00445 at 87.783 kb on - strand, within PS417_00445 at 87.783 kb on - strand, within PS417_00445 at 87.783 kb on - strand, within PS417_00445 at 87.783 kb on - strand, within PS417_00445 at 88.073 kb on + strand, within PS417_00445 at 88.076 kb on + strand, within PS417_00445 at 88.150 kb on + strand, within PS417_00445 at 88.151 kb on - strand, within PS417_00445 at 88.186 kb on - strand, within PS417_00445 at 88.186 kb on - strand, within PS417_00445 at 88.296 kb on - strand, within PS417_00445 at 88.428 kb on - strand, within PS417_00445 at 88.428 kb on - strand, within PS417_00445 at 88.962 kb on + strand, within PS417_00445 at 88.962 kb on + strand, within PS417_00445 at 88.963 kb on - strand at 89.068 kb on + strand at 89.173 kb on + strand at 89.174 kb on - strand at 89.216 kb on - strand at 89.216 kb on - strand at 89.220 kb on + strand at 89.220 kb on + strand at 89.221 kb on - strand at 89.221 kb on - strand at 89.221 kb on - strand at 89.236 kb on + strand at 89.237 kb on - strand at 89.319 kb on + strand at 89.469 kb on - strand, within PS417_00450 at 89.501 kb on - strand, within PS417_00450 at 89.554 kb on + strand, within PS417_00450 at 89.554 kb on + strand, within PS417_00450 at 89.555 kb on - strand, within PS417_00450 at 90.153 kb on + strand, within PS417_00450 at 90.154 kb on - strand, within PS417_00450
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 86,522 + -0.7 86,522 + +0.1 86,523 - +0.7 86,523 - +0.4 86,564 + -0.1 86,641 + +2.3 86,642 - -0.0 86,642 - +0.1 86,642 - -0.1 86,667 + +0.0 86,667 + +0.5 86,874 + PS417_00440 0.28 +0.3 86,874 + PS417_00440 0.28 +1.0 86,874 + PS417_00440 0.28 +0.6 86,874 + PS417_00440 0.28 +0.5 86,875 - PS417_00440 0.28 +0.5 86,875 - PS417_00440 0.28 +0.1 87,016 - PS417_00440 0.46 +0.3 87,039 - PS417_00440 0.49 +0.2 87,039 - PS417_00440 0.49 +0.6 87,190 + PS417_00440 0.69 +0.5 87,231 + PS417_00440 0.74 -0.0 87,231 + PS417_00440 0.74 +0.7 87,232 - PS417_00440 0.74 -0.0 87,232 - PS417_00440 0.74 -0.2 87,447 + -0.7 87,447 + -1.9 87,447 + -0.8 87,448 - -1.3 87,448 - -0.7 87,448 - -1.8 87,448 - -1.1 87,478 - -1.7 87,600 + +0.9 87,753 + PS417_00445 0.18 -1.7 87,754 - PS417_00445 0.18 -1.0 87,783 - PS417_00445 0.20 -0.4 87,783 - PS417_00445 0.20 -0.6 87,783 - PS417_00445 0.20 +0.4 87,783 - PS417_00445 0.20 -1.6 88,073 + PS417_00445 0.36 -0.0 88,076 + PS417_00445 0.36 -0.2 88,150 + PS417_00445 0.40 -0.0 88,151 - PS417_00445 0.40 -1.0 88,186 - PS417_00445 0.42 -0.3 88,186 - PS417_00445 0.42 -0.5 88,296 - PS417_00445 0.49 +0.1 88,428 - PS417_00445 0.56 -0.1 88,428 - PS417_00445 0.56 -0.3 88,962 + PS417_00445 0.86 +0.1 88,962 + PS417_00445 0.86 -0.0 88,963 - -1.0 89,068 + +0.1 89,173 + +0.1 89,174 - +0.2 89,216 - -0.1 89,216 - +0.6 89,220 + +0.0 89,220 + +0.3 89,221 - +0.3 89,221 - +0.6 89,221 - -0.0 89,236 + +1.5 89,237 - -0.7 89,319 + +0.4 89,469 - PS417_00450 0.18 +0.2 89,501 - PS417_00450 0.20 -1.4 89,554 + PS417_00450 0.24 -0.0 89,554 + PS417_00450 0.24 +0.2 89,555 - PS417_00450 0.24 -0.1 90,153 + PS417_00450 0.69 +0.5 90,154 - PS417_00450 0.69 +0.7
Or see this region's nucleotide sequence