Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24705 and PS417_24710 are separated by 180 nucleotides PS417_24710 and PS417_24715 overlap by 1 nucleotides
PS417_24705: PS417_24705 - D-alanyl-D-alanine carboxypeptidase, at 5,359,703 to 5,360,860
_24705
PS417_24710: PS417_24710 - lipoprotein, at 5,361,041 to 5,362,045
_24710
PS417_24715: PS417_24715 - lytic transglycosylase, at 5,362,045 to 5,363,055
_24715
Position (kb) 5361
5362
5363 Strain fitness (log2 ratio) -1
0
1
2 at 5360.279 kb on - strand, within PS417_24705 at 5360.283 kb on + strand, within PS417_24705 at 5360.283 kb on + strand, within PS417_24705 at 5360.650 kb on - strand, within PS417_24705 at 5360.880 kb on - strand at 5360.909 kb on - strand at 5360.977 kb on - strand at 5361.038 kb on + strand at 5361.038 kb on + strand at 5361.039 kb on - strand at 5361.039 kb on - strand at 5361.043 kb on - strand at 5361.086 kb on + strand at 5361.086 kb on + strand at 5361.087 kb on - strand at 5361.087 kb on - strand at 5361.140 kb on + strand at 5361.141 kb on - strand at 5361.182 kb on - strand, within PS417_24710 at 5361.203 kb on - strand, within PS417_24710 at 5361.319 kb on + strand, within PS417_24710 at 5361.622 kb on - strand, within PS417_24710 at 5361.622 kb on - strand, within PS417_24710 at 5361.673 kb on - strand, within PS417_24710 at 5361.673 kb on - strand, within PS417_24710 at 5361.745 kb on - strand, within PS417_24710 at 5361.745 kb on - strand, within PS417_24710 at 5361.772 kb on - strand, within PS417_24710 at 5361.793 kb on - strand, within PS417_24710 at 5362.027 kb on - strand at 5362.046 kb on + strand at 5362.104 kb on - strand at 5362.104 kb on - strand at 5362.104 kb on - strand at 5362.104 kb on - strand at 5362.120 kb on - strand at 5362.122 kb on - strand at 5362.122 kb on - strand at 5362.206 kb on + strand, within PS417_24715 at 5362.207 kb on - strand, within PS417_24715 at 5362.207 kb on - strand, within PS417_24715 at 5362.207 kb on - strand, within PS417_24715 at 5362.370 kb on + strand, within PS417_24715 at 5362.370 kb on + strand, within PS417_24715 at 5362.413 kb on + strand, within PS417_24715 at 5362.413 kb on + strand, within PS417_24715 at 5362.414 kb on - strand at 5362.414 kb on - strand, within PS417_24715 at 5362.414 kb on - strand, within PS417_24715 at 5362.419 kb on + strand, within PS417_24715 at 5362.420 kb on - strand, within PS417_24715 at 5362.420 kb on - strand, within PS417_24715 at 5362.800 kb on - strand, within PS417_24715 at 5362.961 kb on + strand at 5362.961 kb on + strand at 5362.962 kb on - strand at 5362.962 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 5,360,279 - PS417_24705 0.50 -0.1 5,360,283 + PS417_24705 0.50 -0.2 5,360,283 + PS417_24705 0.50 +0.9 5,360,650 - PS417_24705 0.82 +1.8 5,360,880 - -0.1 5,360,909 - -0.2 5,360,977 - -0.2 5,361,038 + +1.1 5,361,038 + +2.2 5,361,039 - -0.3 5,361,039 - -0.1 5,361,043 - +0.1 5,361,086 + +0.8 5,361,086 + +1.0 5,361,087 - +0.3 5,361,087 - +0.1 5,361,140 + +1.8 5,361,141 - +0.4 5,361,182 - PS417_24710 0.14 +0.1 5,361,203 - PS417_24710 0.16 +0.3 5,361,319 + PS417_24710 0.28 +0.3 5,361,622 - PS417_24710 0.58 +0.7 5,361,622 - PS417_24710 0.58 +0.2 5,361,673 - PS417_24710 0.63 +0.6 5,361,673 - PS417_24710 0.63 +0.6 5,361,745 - PS417_24710 0.70 +0.2 5,361,745 - PS417_24710 0.70 +0.0 5,361,772 - PS417_24710 0.73 +0.3 5,361,793 - PS417_24710 0.75 +0.7 5,362,027 - +0.7 5,362,046 + +2.1 5,362,104 - -0.1 5,362,104 - +0.5 5,362,104 - -0.1 5,362,104 - -0.1 5,362,120 - -0.2 5,362,122 - -0.2 5,362,122 - -0.1 5,362,206 + PS417_24715 0.16 +0.3 5,362,207 - PS417_24715 0.16 -0.0 5,362,207 - PS417_24715 0.16 -0.1 5,362,207 - PS417_24715 0.16 -0.2 5,362,370 + PS417_24715 0.32 +0.8 5,362,370 + PS417_24715 0.32 +0.1 5,362,413 + PS417_24715 0.36 +0.2 5,362,413 + PS417_24715 0.36 +0.1 5,362,414 - +0.8 5,362,414 - PS417_24715 0.36 -0.1 5,362,414 - PS417_24715 0.36 -0.2 5,362,419 + PS417_24715 0.37 -0.7 5,362,420 - PS417_24715 0.37 -0.3 5,362,420 - PS417_24715 0.37 -0.1 5,362,800 - PS417_24715 0.75 +0.0 5,362,961 + +0.6 5,362,961 + -0.1 5,362,962 - -0.4 5,362,962 - -0.1
Or see this region's nucleotide sequence