Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02100 and PS417_02105 overlap by 4 nucleotides PS417_02105 and PS417_02110 are separated by 9 nucleotides PS417_02110 and PS417_02115 overlap by 23 nucleotides PS417_02115 and PS417_02120 overlap by 25 nucleotides PS417_02120 and PS417_02125 are separated by 304 nucleotides
PS417_02100: PS417_02100 - membrane protein, at 470,726 to 471,271
_02100
PS417_02105: PS417_02105 - acyl carrier protein, at 471,268 to 471,522
_02105
PS417_02110: PS417_02110 - acyl carrier protein, at 471,532 to 471,801
_02110
PS417_02115: PS417_02115 - acyltransferase, at 471,779 to 472,591
_02115
PS417_02120: PS417_02120 - 3-oxoacyl-ACP synthase, at 472,567 to 473,286
_02120
PS417_02125: PS417_02125 - cobalamin biosynthesis protein CobQ, at 473,591 to 474,361
_02125
Position (kb) 471
472
473 Strain fitness (log2 ratio) -2
-1
0
1 at 470.811 kb on + strand, within PS417_02100 at 470.811 kb on + strand, within PS417_02100 at 470.913 kb on + strand, within PS417_02100 at 470.913 kb on + strand, within PS417_02100 at 470.914 kb on - strand, within PS417_02100 at 470.983 kb on - strand, within PS417_02100 at 471.210 kb on + strand, within PS417_02100 at 471.210 kb on + strand, within PS417_02100 at 471.211 kb on - strand, within PS417_02100 at 471.211 kb on - strand, within PS417_02100 at 471.294 kb on - strand, within PS417_02105 at 471.375 kb on + strand, within PS417_02105 at 471.375 kb on + strand, within PS417_02105 at 471.504 kb on + strand at 471.769 kb on + strand, within PS417_02110 at 471.825 kb on + strand at 471.899 kb on + strand, within PS417_02115 at 471.899 kb on + strand, within PS417_02115 at 471.899 kb on + strand, within PS417_02115 at 471.900 kb on - strand, within PS417_02115 at 471.900 kb on - strand, within PS417_02115 at 471.901 kb on + strand, within PS417_02115 at 472.172 kb on - strand, within PS417_02115 at 472.174 kb on - strand, within PS417_02115 at 472.409 kb on - strand, within PS417_02115 at 472.409 kb on - strand, within PS417_02115 at 472.546 kb on - strand at 472.546 kb on - strand at 473.296 kb on - strand at 473.397 kb on - strand at 473.403 kb on + strand at 473.403 kb on + strand at 473.448 kb on + strand at 473.449 kb on - strand at 473.554 kb on + strand at 473.575 kb on - strand at 473.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 470,811 + PS417_02100 0.16 -0.7 470,811 + PS417_02100 0.16 -0.2 470,913 + PS417_02100 0.34 -0.2 470,913 + PS417_02100 0.34 +0.5 470,914 - PS417_02100 0.34 +0.1 470,983 - PS417_02100 0.47 -0.3 471,210 + PS417_02100 0.89 -0.0 471,210 + PS417_02100 0.89 -0.2 471,211 - PS417_02100 0.89 -0.8 471,211 - PS417_02100 0.89 -0.2 471,294 - PS417_02105 0.10 -0.2 471,375 + PS417_02105 0.42 +0.4 471,375 + PS417_02105 0.42 -0.1 471,504 + -0.3 471,769 + PS417_02110 0.88 +0.2 471,825 + -0.2 471,899 + PS417_02115 0.15 +0.3 471,899 + PS417_02115 0.15 -0.3 471,899 + PS417_02115 0.15 -0.9 471,900 - PS417_02115 0.15 -0.0 471,900 - PS417_02115 0.15 -0.1 471,901 + PS417_02115 0.15 -0.2 472,172 - PS417_02115 0.48 -0.6 472,174 - PS417_02115 0.49 -0.5 472,409 - PS417_02115 0.77 +0.1 472,409 - PS417_02115 0.77 -2.2 472,546 - +1.1 472,546 - +0.3 473,296 - -0.3 473,397 - -0.6 473,403 + +0.6 473,403 + -0.2 473,448 + +0.3 473,449 - +0.2 473,554 + -0.0 473,575 - +1.0 473,575 - -0.1
Or see this region's nucleotide sequence