Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11785 and PS417_11790 are separated by 24 nucleotides PS417_11790 and PS417_11795 overlap by 4 nucleotides
PS417_11785: PS417_11785 - glutamine synthetase, at 2,564,521 to 2,565,879
_11785
PS417_11790: PS417_11790 - ABC transporter substrate-binding protein, at 2,565,904 to 2,566,992
_11790
PS417_11795: PS417_11795 - FAD-dependent oxidoreductase, at 2,566,989 to 2,568,281
_11795
Position (kb)
2565
2566
2567 Strain fitness (log2 ratio)
-1
0
1 at 2564.950 kb on + strand, within PS417_11785 at 2565.410 kb on - strand, within PS417_11785 at 2565.616 kb on - strand, within PS417_11785 at 2565.632 kb on - strand, within PS417_11785 at 2566.003 kb on + strand at 2566.003 kb on + strand at 2566.005 kb on + strand at 2566.283 kb on - strand, within PS417_11790 at 2566.310 kb on - strand, within PS417_11790 at 2566.460 kb on - strand, within PS417_11790 at 2566.460 kb on - strand, within PS417_11790 at 2566.499 kb on - strand, within PS417_11790 at 2566.499 kb on - strand, within PS417_11790 at 2566.537 kb on + strand at 2566.547 kb on - strand, within PS417_11790 at 2566.590 kb on + strand at 2566.590 kb on + strand, within PS417_11790 at 2566.915 kb on + strand at 2567.019 kb on + strand at 2567.019 kb on + strand at 2567.020 kb on - strand at 2567.479 kb on + strand, within PS417_11795 at 2567.479 kb on + strand, within PS417_11795 at 2567.479 kb on + strand, within PS417_11795 at 2567.505 kb on + strand, within PS417_11795 at 2567.763 kb on + strand, within PS417_11795 at 2567.764 kb on - strand, within PS417_11795 at 2567.857 kb on - strand, within PS417_11795 at 2567.940 kb on + strand, within PS417_11795 at 2567.941 kb on - strand, within PS417_11795 at 2567.941 kb on - strand, within PS417_11795
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 2,564,950 + PS417_11785 0.32 +0.1 2,565,410 - PS417_11785 0.65 +0.9 2,565,616 - PS417_11785 0.81 -0.0 2,565,632 - PS417_11785 0.82 +0.5 2,566,003 + -1.4 2,566,003 + -0.4 2,566,005 + +0.1 2,566,283 - PS417_11790 0.35 -0.1 2,566,310 - PS417_11790 0.37 -0.4 2,566,460 - PS417_11790 0.51 -0.3 2,566,460 - PS417_11790 0.51 -0.3 2,566,499 - PS417_11790 0.55 -0.2 2,566,499 - PS417_11790 0.55 -0.7 2,566,537 + +0.0 2,566,547 - PS417_11790 0.59 -0.7 2,566,590 + +0.9 2,566,590 + PS417_11790 0.63 -1.0 2,566,915 + -0.2 2,567,019 + -0.1 2,567,019 + -0.0 2,567,020 - +0.5 2,567,479 + PS417_11795 0.38 +0.3 2,567,479 + PS417_11795 0.38 -1.5 2,567,479 + PS417_11795 0.38 +0.7 2,567,505 + PS417_11795 0.40 +0.1 2,567,763 + PS417_11795 0.60 +0.4 2,567,764 - PS417_11795 0.60 -0.2 2,567,857 - PS417_11795 0.67 -0.5 2,567,940 + PS417_11795 0.74 +1.0 2,567,941 - PS417_11795 0.74 +0.7 2,567,941 - PS417_11795 0.74 +0.1
Or see this region's nucleotide sequence