Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09300 and PS417_09305 are separated by 319 nucleotides PS417_09305 and PS417_09310 are separated by 94 nucleotides PS417_09310 and PS417_09315 overlap by 4 nucleotides PS417_09315 and PS417_09320 overlap by 4 nucleotides
PS417_09300: PS417_09300 - peptidylprolyl isomerase, at 2,018,579 to 2,019,310
_09300
PS417_09305: PS417_09305 - hypothetical protein, at 2,019,630 to 2,020,088
_09305
PS417_09310: PS417_09310 - Cro/Cl family transcriptional regulator, at 2,020,183 to 2,020,524
_09310
PS417_09315: PS417_09315 - hypothetical protein, at 2,020,521 to 2,020,892
_09315
PS417_09320: PS417_09320 - diguanylate phosphodiesterase, at 2,020,889 to 2,022,658
_09320
Position (kb)
2019
2020
2021 Strain fitness (log2 ratio)
-1
0
1 at 2018.960 kb on + strand, within PS417_09300 at 2019.164 kb on + strand, within PS417_09300 at 2019.164 kb on + strand, within PS417_09300 at 2019.165 kb on - strand, within PS417_09300 at 2019.271 kb on + strand at 2019.309 kb on - strand at 2019.325 kb on + strand at 2019.325 kb on + strand at 2019.326 kb on - strand at 2019.326 kb on - strand at 2019.326 kb on - strand at 2019.326 kb on - strand at 2019.326 kb on - strand at 2019.375 kb on + strand at 2019.376 kb on - strand at 2019.420 kb on - strand at 2019.420 kb on - strand at 2019.431 kb on - strand at 2019.431 kb on - strand at 2019.431 kb on - strand at 2019.431 kb on - strand at 2019.433 kb on - strand at 2019.433 kb on - strand at 2019.433 kb on - strand at 2019.532 kb on + strand at 2019.801 kb on + strand, within PS417_09305 at 2019.802 kb on - strand, within PS417_09305 at 2019.867 kb on + strand, within PS417_09305 at 2019.868 kb on - strand, within PS417_09305 at 2020.094 kb on + strand at 2020.094 kb on + strand at 2020.094 kb on + strand at 2020.094 kb on + strand at 2020.095 kb on - strand at 2020.096 kb on + strand at 2020.149 kb on + strand at 2020.156 kb on + strand at 2020.156 kb on + strand at 2020.157 kb on - strand at 2020.166 kb on + strand at 2020.528 kb on + strand at 2020.532 kb on + strand at 2020.533 kb on - strand at 2020.890 kb on + strand at 2020.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 2,018,960 + PS417_09300 0.52 -0.6 2,019,164 + PS417_09300 0.80 +0.0 2,019,164 + PS417_09300 0.80 -0.2 2,019,165 - PS417_09300 0.80 -0.9 2,019,271 + +0.3 2,019,309 - -0.4 2,019,325 + +0.3 2,019,325 + +0.2 2,019,326 - -0.2 2,019,326 - -0.5 2,019,326 - -0.2 2,019,326 - +0.3 2,019,326 - +0.2 2,019,375 + -0.3 2,019,376 - -0.2 2,019,420 - -0.1 2,019,420 - -0.2 2,019,431 - -0.1 2,019,431 - -0.3 2,019,431 - -0.5 2,019,431 - -0.2 2,019,433 - -0.5 2,019,433 - -0.2 2,019,433 - -0.2 2,019,532 + +0.3 2,019,801 + PS417_09305 0.37 -0.3 2,019,802 - PS417_09305 0.37 -0.3 2,019,867 + PS417_09305 0.52 -0.5 2,019,868 - PS417_09305 0.52 -0.4 2,020,094 + +0.4 2,020,094 + +0.0 2,020,094 + -0.5 2,020,094 + -0.3 2,020,095 - -0.1 2,020,096 + +0.1 2,020,149 + +0.3 2,020,156 + -0.2 2,020,156 + -0.1 2,020,157 - +0.3 2,020,166 + +0.3 2,020,528 + -0.7 2,020,532 + +0.4 2,020,533 - -0.1 2,020,890 + -0.2 2,020,937 - -0.1
Or see this region's nucleotide sequence