Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08735 and PS417_08740 are separated by 278 nucleotides PS417_08740 and PS417_08745 are separated by 24 nucleotides PS417_08745 and PS417_08750 are separated by 319 nucleotides PS417_08750 and PS417_08755 are separated by 11 nucleotides
PS417_08735: PS417_08735 - hypothetical protein, at 1,902,276 to 1,902,671
_08735
PS417_08740: PS417_08740 - ribose-phosphate pyrophosphokinase, at 1,902,950 to 1,903,522
_08740
PS417_08745: PS417_08745 - hypothetical protein, at 1,903,547 to 1,904,023
_08745
PS417_08750: PS417_08750 - hypothetical protein, at 1,904,343 to 1,904,531
_08750
PS417_08755: PS417_08755 - CopG family transcriptional regulator, at 1,904,543 to 1,904,947
_08755
Position (kb) 1903
1904
1905 Strain fitness (log2 ratio) -1
0
1 at 1902.547 kb on - strand, within PS417_08735 at 1902.547 kb on - strand, within PS417_08735 at 1902.561 kb on + strand, within PS417_08735 at 1902.561 kb on + strand, within PS417_08735 at 1902.562 kb on - strand, within PS417_08735 at 1902.562 kb on - strand, within PS417_08735 at 1902.843 kb on - strand at 1902.843 kb on - strand at 1902.856 kb on + strand at 1902.857 kb on - strand at 1902.968 kb on + strand at 1903.323 kb on + strand, within PS417_08740 at 1903.324 kb on - strand, within PS417_08740 at 1903.468 kb on + strand at 1903.468 kb on + strand at 1903.468 kb on + strand at 1903.468 kb on + strand at 1903.468 kb on + strand at 1903.469 kb on - strand at 1903.469 kb on - strand at 1903.539 kb on + strand at 1903.540 kb on - strand at 1903.540 kb on - strand at 1903.540 kb on - strand at 1903.550 kb on + strand at 1903.551 kb on - strand at 1903.557 kb on - strand at 1903.635 kb on + strand, within PS417_08745 at 1903.635 kb on + strand, within PS417_08745 at 1903.636 kb on - strand, within PS417_08745 at 1903.636 kb on - strand, within PS417_08745 at 1903.648 kb on - strand, within PS417_08745 at 1903.651 kb on + strand, within PS417_08745 at 1903.881 kb on - strand, within PS417_08745 at 1903.924 kb on - strand, within PS417_08745 at 1903.924 kb on - strand at 1904.009 kb on + strand at 1904.010 kb on - strand at 1904.067 kb on + strand at 1904.090 kb on + strand at 1904.090 kb on + strand at 1904.090 kb on + strand at 1904.090 kb on + strand at 1904.091 kb on - strand at 1904.102 kb on - strand at 1904.110 kb on - strand at 1904.130 kb on - strand at 1904.130 kb on - strand at 1904.130 kb on - strand at 1904.130 kb on - strand at 1904.252 kb on - strand at 1904.417 kb on + strand, within PS417_08750 at 1904.846 kb on + strand, within PS417_08755 at 1904.957 kb on - strand at 1904.964 kb on - strand at 1904.985 kb on + strand at 1904.985 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 1,902,547 - PS417_08735 0.68 -0.3 1,902,547 - PS417_08735 0.68 -0.1 1,902,561 + PS417_08735 0.72 -0.4 1,902,561 + PS417_08735 0.72 -0.6 1,902,562 - PS417_08735 0.72 -0.5 1,902,562 - PS417_08735 0.72 -0.1 1,902,843 - +0.6 1,902,843 - +0.1 1,902,856 + +0.0 1,902,857 - -0.4 1,902,968 + -0.6 1,903,323 + PS417_08740 0.65 -0.2 1,903,324 - PS417_08740 0.65 +0.6 1,903,468 + +0.0 1,903,468 + -0.0 1,903,468 + -0.1 1,903,468 + -0.0 1,903,468 + +0.4 1,903,469 - +0.1 1,903,469 - +0.2 1,903,539 + -0.0 1,903,540 - +1.3 1,903,540 - -0.1 1,903,540 - -0.1 1,903,550 + -0.2 1,903,551 - -0.3 1,903,557 - -0.7 1,903,635 + PS417_08745 0.18 -0.3 1,903,635 + PS417_08745 0.18 -0.5 1,903,636 - PS417_08745 0.19 +0.0 1,903,636 - PS417_08745 0.19 -1.4 1,903,648 - PS417_08745 0.21 +0.0 1,903,651 + PS417_08745 0.22 -0.3 1,903,881 - PS417_08745 0.70 -0.1 1,903,924 - PS417_08745 0.79 -1.4 1,903,924 - -0.1 1,904,009 + -0.1 1,904,010 - -0.3 1,904,067 + +0.3 1,904,090 + +0.9 1,904,090 + -0.6 1,904,090 + -0.2 1,904,090 + -0.3 1,904,091 - -0.3 1,904,102 - +0.0 1,904,110 - -0.3 1,904,130 - -0.5 1,904,130 - -0.2 1,904,130 - -0.1 1,904,130 - +0.0 1,904,252 - -0.5 1,904,417 + PS417_08750 0.39 +0.7 1,904,846 + PS417_08755 0.75 -0.6 1,904,957 - +0.0 1,904,964 - -0.1 1,904,985 + +0.0 1,904,985 + +0.2
Or see this region's nucleotide sequence