Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07135 and PS417_07140 are separated by 65 nucleotides PS417_07140 and PS417_07145 are separated by 94 nucleotides PS417_07145 and PS417_07150 are separated by 30 nucleotides
PS417_07135: PS417_07135 - serine peptidase, at 1,577,421 to 1,578,860
_07135
PS417_07140: PS417_07140 - peptidase M48, at 1,578,926 to 1,580,359
_07140
PS417_07145: PS417_07145 - response regulator SirA, at 1,580,454 to 1,580,693
_07145
PS417_07150: PS417_07150 - permase, at 1,580,724 to 1,581,794
_07150
Position (kb)
1578
1579
1580
1581 Strain fitness (log2 ratio)
-1
0
1 at 1578.859 kb on - strand at 1578.927 kb on + strand at 1578.928 kb on - strand at 1578.928 kb on - strand at 1578.928 kb on - strand at 1579.146 kb on + strand, within PS417_07140 at 1579.357 kb on - strand, within PS417_07140 at 1579.357 kb on - strand, within PS417_07140 at 1579.357 kb on - strand, within PS417_07140 at 1579.849 kb on + strand, within PS417_07140 at 1580.145 kb on + strand, within PS417_07140 at 1580.239 kb on - strand at 1580.385 kb on + strand at 1580.385 kb on + strand at 1580.385 kb on + strand at 1580.386 kb on - strand at 1580.418 kb on - strand at 1580.422 kb on + strand at 1580.737 kb on + strand at 1580.757 kb on + strand at 1580.758 kb on - strand at 1580.872 kb on - strand, within PS417_07150 at 1580.875 kb on + strand, within PS417_07150 at 1580.875 kb on + strand, within PS417_07150 at 1580.875 kb on + strand, within PS417_07150 at 1581.216 kb on + strand, within PS417_07150 at 1581.216 kb on + strand, within PS417_07150 at 1581.217 kb on - strand, within PS417_07150 at 1581.357 kb on + strand, within PS417_07150 at 1581.358 kb on - strand, within PS417_07150 at 1581.358 kb on - strand, within PS417_07150
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 1,578,859 - -0.2 1,578,927 + -0.1 1,578,928 - -1.1 1,578,928 - +0.5 1,578,928 - +0.7 1,579,146 + PS417_07140 0.15 -0.3 1,579,357 - PS417_07140 0.30 -0.0 1,579,357 - PS417_07140 0.30 -1.0 1,579,357 - PS417_07140 0.30 +0.0 1,579,849 + PS417_07140 0.64 +0.7 1,580,145 + PS417_07140 0.85 -0.3 1,580,239 - -0.6 1,580,385 + +0.0 1,580,385 + +0.2 1,580,385 + +0.0 1,580,386 - -0.8 1,580,418 - +0.4 1,580,422 + -0.5 1,580,737 + -0.3 1,580,757 + -1.6 1,580,758 - +0.5 1,580,872 - PS417_07150 0.14 -0.2 1,580,875 + PS417_07150 0.14 +0.7 1,580,875 + PS417_07150 0.14 -0.1 1,580,875 + PS417_07150 0.14 +0.2 1,581,216 + PS417_07150 0.46 +0.1 1,581,216 + PS417_07150 0.46 -0.2 1,581,217 - PS417_07150 0.46 -0.1 1,581,357 + PS417_07150 0.59 +0.2 1,581,358 - PS417_07150 0.59 +0.2 1,581,358 - PS417_07150 0.59 -0.2
Or see this region's nucleotide sequence