Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00530 and PS417_00535 are separated by 26 nucleotides PS417_00535 and PS417_00540 are separated by 80 nucleotides PS417_00540 and PS417_00545 are separated by 136 nucleotides PS417_00545 and PS417_00550 are separated by 263 nucleotides PS417_00550 and PS417_00555 are separated by 317 nucleotides
PS417_00530: PS417_00530 - hypothetical protein, at 107,882 to 108,202
_00530
PS417_00535: PS417_00535 - Holliday junction resolvase, at 108,229 to 108,537
_00535
PS417_00540: PS417_00540 - hypothetical protein, at 108,618 to 108,938
_00540
PS417_00545: PS417_00545 - hypothetical protein, at 109,075 to 109,260
_00545
PS417_00550: PS417_00550 - hypothetical protein, at 109,524 to 109,739
_00550
PS417_00555: PS417_00555 - acetyl-CoA hydrolase, at 110,057 to 111,550
_00555
Position (kb)
109
110 Strain fitness (log2 ratio)
-1
0
1 at 108.196 kb on + strand at 108.197 kb on - strand at 108.197 kb on - strand at 108.197 kb on - strand at 108.300 kb on - strand, within PS417_00535 at 108.300 kb on - strand, within PS417_00535 at 108.429 kb on - strand, within PS417_00535 at 108.514 kb on + strand at 108.576 kb on - strand at 108.623 kb on + strand at 108.953 kb on - strand at 108.953 kb on - strand at 108.953 kb on - strand at 108.965 kb on - strand at 109.003 kb on + strand at 109.003 kb on + strand at 109.004 kb on - strand at 109.006 kb on + strand at 109.006 kb on + strand at 109.007 kb on - strand at 109.033 kb on + strand at 109.237 kb on - strand, within PS417_00545 at 109.239 kb on - strand, within PS417_00545 at 109.239 kb on - strand, within PS417_00545 at 109.324 kb on + strand at 109.324 kb on + strand at 109.324 kb on + strand at 109.325 kb on - strand at 109.349 kb on + strand at 109.372 kb on + strand at 109.373 kb on - strand at 109.693 kb on - strand, within PS417_00550 at 109.730 kb on + strand at 109.731 kb on - strand at 109.768 kb on + strand at 109.773 kb on - strand at 109.773 kb on - strand at 109.781 kb on - strand at 109.823 kb on + strand at 109.823 kb on + strand at 109.861 kb on + strand at 109.863 kb on + strand at 109.872 kb on + strand at 109.873 kb on - strand at 109.942 kb on + strand at 109.942 kb on + strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 109.943 kb on - strand at 110.051 kb on + strand at 110.052 kb on - strand at 110.052 kb on - strand at 110.163 kb on + strand at 110.203 kb on + strand at 110.204 kb on - strand at 110.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 108,196 + +0.5 108,197 - +0.3 108,197 - +0.0 108,197 - +0.0 108,300 - PS417_00535 0.23 -1.6 108,300 - PS417_00535 0.23 -0.1 108,429 - PS417_00535 0.65 -0.0 108,514 + -0.2 108,576 - -0.3 108,623 + +0.2 108,953 - +0.2 108,953 - -0.2 108,953 - -0.3 108,965 - +0.0 109,003 + +0.0 109,003 + +0.5 109,004 - +0.6 109,006 + +0.0 109,006 + -0.4 109,007 - +0.2 109,033 + -0.2 109,237 - PS417_00545 0.87 +0.5 109,239 - PS417_00545 0.88 +0.4 109,239 - PS417_00545 0.88 -0.1 109,324 + +0.8 109,324 + -0.3 109,324 + +0.3 109,325 - -0.5 109,349 + +0.1 109,372 + +0.3 109,373 - +0.3 109,693 - PS417_00550 0.78 +0.1 109,730 + +0.3 109,731 - -0.5 109,768 + +0.1 109,773 - -0.0 109,773 - -0.2 109,781 - -0.1 109,823 + +0.1 109,823 + +0.2 109,861 + +0.5 109,863 + +0.4 109,872 + +0.2 109,873 - -0.1 109,942 + -0.1 109,942 + -0.4 109,943 - -0.2 109,943 - -0.2 109,943 - -0.5 109,943 - +0.4 109,943 - -0.5 109,943 - -0.1 109,943 - -0.4 109,943 - +0.3 109,943 - +0.7 109,943 - -0.2 110,051 + +0.2 110,052 - +0.8 110,052 - +0.1 110,163 + -0.0 110,203 + +0.3 110,204 - +0.3 110,204 - +1.3
Or see this region's nucleotide sequence