Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05205 and PS417_05210 are separated by 277 nucleotides PS417_05210 and PS417_05215 overlap by 1 nucleotides PS417_05215 and PS417_05220 are separated by 79 nucleotides
PS417_05205: PS417_05205 - pyridoxine 5'-phosphate synthase, at 1,155,078 to 1,155,821
_05205
PS417_05210: PS417_05210 - copper oxidase, at 1,156,099 to 1,157,475
_05210
PS417_05215: PS417_05215 - zinc-binding protein, at 1,157,475 to 1,157,972
_05215
PS417_05220: PS417_05220 - tRNA methyltransferase, at 1,158,052 to 1,159,008
_05220
Position (kb)
1156
1157
1158 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1155.820 kb on - strand at 1155.820 kb on - strand at 1155.835 kb on - strand at 1155.835 kb on - strand at 1155.904 kb on - strand at 1155.904 kb on - strand at 1155.904 kb on - strand at 1155.904 kb on - strand at 1155.904 kb on - strand at 1155.980 kb on + strand at 1155.981 kb on - strand at 1155.981 kb on - strand at 1155.985 kb on - strand at 1155.985 kb on - strand at 1156.050 kb on + strand at 1156.050 kb on + strand at 1156.085 kb on + strand at 1156.435 kb on + strand, within PS417_05210 at 1156.435 kb on + strand, within PS417_05210 at 1156.435 kb on + strand, within PS417_05210 at 1156.436 kb on - strand, within PS417_05210 at 1156.468 kb on + strand, within PS417_05210 at 1156.507 kb on + strand, within PS417_05210 at 1156.508 kb on - strand, within PS417_05210 at 1156.513 kb on + strand, within PS417_05210 at 1156.514 kb on - strand, within PS417_05210 at 1156.604 kb on - strand, within PS417_05210 at 1156.804 kb on + strand, within PS417_05210 at 1156.805 kb on - strand, within PS417_05210 at 1156.840 kb on + strand, within PS417_05210 at 1156.888 kb on + strand, within PS417_05210 at 1157.230 kb on + strand, within PS417_05210 at 1157.231 kb on - strand, within PS417_05210 at 1157.233 kb on - strand, within PS417_05210 at 1157.347 kb on + strand at 1157.347 kb on + strand at 1157.347 kb on + strand at 1157.348 kb on - strand at 1158.149 kb on + strand, within PS417_05220 at 1158.149 kb on + strand, within PS417_05220 at 1158.212 kb on - strand, within PS417_05220 at 1158.275 kb on - strand, within PS417_05220 at 1158.275 kb on - strand, within PS417_05220 at 1158.280 kb on - strand, within PS417_05220 at 1158.280 kb on - strand, within PS417_05220 at 1158.423 kb on - strand, within PS417_05220
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 2 remove 1,155,820 - +0.0 1,155,820 - -0.0 1,155,835 - +0.3 1,155,835 - -0.7 1,155,904 - -0.0 1,155,904 - +0.1 1,155,904 - -0.2 1,155,904 - +1.5 1,155,904 - +0.5 1,155,980 + -0.1 1,155,981 - +0.5 1,155,981 - +0.8 1,155,985 - -0.1 1,155,985 - -0.1 1,156,050 + +0.4 1,156,050 + -0.2 1,156,085 + -0.3 1,156,435 + PS417_05210 0.24 -0.4 1,156,435 + PS417_05210 0.24 +1.0 1,156,435 + PS417_05210 0.24 -0.3 1,156,436 - PS417_05210 0.24 -1.0 1,156,468 + PS417_05210 0.27 -1.0 1,156,507 + PS417_05210 0.30 +0.5 1,156,508 - PS417_05210 0.30 +0.6 1,156,513 + PS417_05210 0.30 -1.2 1,156,514 - PS417_05210 0.30 -3.1 1,156,604 - PS417_05210 0.37 -1.4 1,156,804 + PS417_05210 0.51 -0.1 1,156,805 - PS417_05210 0.51 -1.3 1,156,840 + PS417_05210 0.54 -0.2 1,156,888 + PS417_05210 0.57 -0.9 1,157,230 + PS417_05210 0.82 +0.2 1,157,231 - PS417_05210 0.82 -0.4 1,157,233 - PS417_05210 0.82 +0.3 1,157,347 + -0.3 1,157,347 + -0.3 1,157,347 + +0.3 1,157,348 - +0.2 1,158,149 + PS417_05220 0.10 +0.8 1,158,149 + PS417_05220 0.10 +0.4 1,158,212 - PS417_05220 0.17 +0.4 1,158,275 - PS417_05220 0.23 -0.1 1,158,275 - PS417_05220 0.23 +0.3 1,158,280 - PS417_05220 0.24 -0.1 1,158,280 - PS417_05220 0.24 +0.5 1,158,423 - PS417_05220 0.39 +0.7
Or see this region's nucleotide sequence