Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17430 and PS417_17435 are separated by 121 nucleotides PS417_17435 and PS417_17440 are separated by 126 nucleotides
PS417_17430: PS417_17430 - aldehyde dehydrogenase, at 3,807,258 to 3,808,778
_17430
PS417_17435: PS417_17435 - dehydrogenase, at 3,808,900 to 3,810,675
_17435
PS417_17440: PS417_17440 - beta-lactamase, at 3,810,802 to 3,811,734
_17440
Position (kb)
3808
3809
3810
3811 Strain fitness (log2 ratio)
-1
0
1 at 3808.400 kb on - strand, within PS417_17430 at 3808.675 kb on + strand at 3808.714 kb on + strand at 3808.715 kb on - strand at 3808.715 kb on - strand at 3808.715 kb on - strand at 3808.718 kb on + strand at 3808.719 kb on - strand at 3808.771 kb on + strand at 3808.772 kb on - strand at 3808.834 kb on + strand at 3808.835 kb on - strand at 3808.835 kb on - strand at 3808.835 kb on - strand at 3808.895 kb on + strand at 3809.037 kb on - strand at 3809.273 kb on + strand, within PS417_17435 at 3809.273 kb on + strand, within PS417_17435 at 3809.274 kb on - strand, within PS417_17435 at 3809.334 kb on - strand, within PS417_17435 at 3809.408 kb on + strand, within PS417_17435 at 3809.408 kb on + strand, within PS417_17435 at 3809.409 kb on - strand, within PS417_17435 at 3809.672 kb on + strand, within PS417_17435 at 3809.706 kb on - strand, within PS417_17435 at 3809.873 kb on + strand, within PS417_17435 at 3809.874 kb on - strand, within PS417_17435 at 3809.874 kb on - strand, within PS417_17435 at 3809.876 kb on + strand at 3809.984 kb on + strand, within PS417_17435 at 3809.984 kb on + strand at 3809.985 kb on - strand, within PS417_17435 at 3809.991 kb on - strand at 3810.050 kb on + strand, within PS417_17435 at 3810.050 kb on + strand at 3810.050 kb on + strand, within PS417_17435 at 3810.051 kb on - strand, within PS417_17435 at 3810.383 kb on + strand, within PS417_17435 at 3810.383 kb on + strand, within PS417_17435 at 3810.383 kb on + strand, within PS417_17435 at 3810.785 kb on - strand at 3810.785 kb on - strand at 3810.995 kb on + strand, within PS417_17440 at 3810.996 kb on - strand, within PS417_17440
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 3,808,400 - PS417_17430 0.75 +0.0 3,808,675 + +0.6 3,808,714 + -0.1 3,808,715 - +0.1 3,808,715 - -0.5 3,808,715 - -0.1 3,808,718 + -0.3 3,808,719 - +0.3 3,808,771 + -0.2 3,808,772 - -0.1 3,808,834 + +0.0 3,808,835 - +0.0 3,808,835 - +0.6 3,808,835 - -0.6 3,808,895 + +1.3 3,809,037 - -0.2 3,809,273 + PS417_17435 0.21 -0.2 3,809,273 + PS417_17435 0.21 +0.2 3,809,274 - PS417_17435 0.21 -0.2 3,809,334 - PS417_17435 0.24 +0.1 3,809,408 + PS417_17435 0.29 -0.7 3,809,408 + PS417_17435 0.29 +0.6 3,809,409 - PS417_17435 0.29 -0.1 3,809,672 + PS417_17435 0.43 -0.4 3,809,706 - PS417_17435 0.45 +0.4 3,809,873 + PS417_17435 0.55 -0.3 3,809,874 - PS417_17435 0.55 +0.3 3,809,874 - PS417_17435 0.55 -0.3 3,809,876 + +0.4 3,809,984 + PS417_17435 0.61 -0.4 3,809,984 + -0.5 3,809,985 - PS417_17435 0.61 +0.3 3,809,991 - +0.5 3,810,050 + PS417_17435 0.65 -0.5 3,810,050 + -0.0 3,810,050 + PS417_17435 0.65 +0.1 3,810,051 - PS417_17435 0.65 +0.3 3,810,383 + PS417_17435 0.84 -0.1 3,810,383 + PS417_17435 0.84 +0.1 3,810,383 + PS417_17435 0.84 -0.1 3,810,785 - -0.9 3,810,785 - +0.2 3,810,995 + PS417_17440 0.21 -0.5 3,810,996 - PS417_17440 0.21 -0.2
Or see this region's nucleotide sequence