Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16710 and PS417_16715 are separated by 18 nucleotides PS417_16715 and PS417_16720 overlap by 4 nucleotides PS417_16720 and PS417_16725 are separated by 129 nucleotides PS417_16725 and PS417_16730 are separated by 137 nucleotides
PS417_16710: PS417_16710 - hydrolase, at 3,646,680 to 3,647,441
_16710
PS417_16715: PS417_16715 - DoxX family protein, at 3,647,460 to 3,647,867
_16715
PS417_16720: PS417_16720 - membrane protein, at 3,647,864 to 3,648,136
_16720
PS417_16725: PS417_16725 - hypothetical protein, at 3,648,266 to 3,648,466
_16725
PS417_16730: PS417_16730 - peroxidase, at 3,648,604 to 3,649,464
_16730
Position (kb)
3647
3648
3649 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3646.881 kb on + strand, within PS417_16710 at 3647.269 kb on + strand at 3647.269 kb on + strand, within PS417_16710 at 3647.435 kb on + strand at 3647.435 kb on + strand at 3647.435 kb on + strand at 3647.436 kb on - strand at 3647.436 kb on - strand at 3647.572 kb on + strand, within PS417_16715 at 3647.709 kb on + strand, within PS417_16715 at 3647.722 kb on + strand, within PS417_16715 at 3647.955 kb on + strand, within PS417_16720 at 3648.163 kb on + strand at 3648.275 kb on + strand at 3648.276 kb on - strand at 3648.312 kb on + strand, within PS417_16725 at 3648.364 kb on - strand at 3648.367 kb on + strand, within PS417_16725 at 3648.367 kb on + strand, within PS417_16725 at 3648.367 kb on + strand, within PS417_16725 at 3648.367 kb on + strand, within PS417_16725 at 3648.368 kb on - strand, within PS417_16725 at 3648.368 kb on - strand, within PS417_16725 at 3648.368 kb on - strand, within PS417_16725 at 3648.368 kb on - strand, within PS417_16725 at 3648.368 kb on - strand, within PS417_16725 at 3648.371 kb on + strand, within PS417_16725 at 3648.372 kb on - strand, within PS417_16725 at 3648.372 kb on - strand, within PS417_16725 at 3648.373 kb on + strand, within PS417_16725 at 3648.461 kb on - strand at 3648.581 kb on - strand at 3648.659 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 2 remove 3,646,881 + PS417_16710 0.26 -0.2 3,647,269 + -0.6 3,647,269 + PS417_16710 0.77 +0.1 3,647,435 + -0.2 3,647,435 + -0.2 3,647,435 + -1.4 3,647,436 - -0.1 3,647,436 - +0.1 3,647,572 + PS417_16715 0.27 +0.2 3,647,709 + PS417_16715 0.61 +0.2 3,647,722 + PS417_16715 0.64 +0.1 3,647,955 + PS417_16720 0.33 +0.5 3,648,163 + +0.2 3,648,275 + -0.3 3,648,276 - -0.3 3,648,312 + PS417_16725 0.23 +0.9 3,648,364 - +2.8 3,648,367 + PS417_16725 0.50 -0.3 3,648,367 + PS417_16725 0.50 -0.2 3,648,367 + PS417_16725 0.50 -0.0 3,648,367 + PS417_16725 0.50 +0.1 3,648,368 - PS417_16725 0.51 +0.3 3,648,368 - PS417_16725 0.51 +0.4 3,648,368 - PS417_16725 0.51 +0.7 3,648,368 - PS417_16725 0.51 +0.5 3,648,368 - PS417_16725 0.51 +0.2 3,648,371 + PS417_16725 0.52 -0.0 3,648,372 - PS417_16725 0.53 +0.0 3,648,372 - PS417_16725 0.53 -0.1 3,648,373 + PS417_16725 0.53 +0.4 3,648,461 - +0.2 3,648,581 - +0.1 3,648,659 + -0.0
Or see this region's nucleotide sequence