Strain Fitness in Pseudomonas simiae WCS417 around PS417_16975
Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1 |
|---|---|---|---|---|---|
| remove | |||||
| 3,707,164 | - | PS417_16970 | 0.49 | -0.4 | |
| 3,707,508 | + | PS417_16970 | 0.68 | +0.8 | |
| 3,707,831 | + | PS417_16970 | 0.87 | +0.3 | |
| 3,707,832 | - | PS417_16970 | 0.87 | -0.1 | |
| 3,708,032 | - | -1.7 | |||
| 3,708,065 | + | +0.1 | |||
| 3,708,066 | - | +0.2 | |||
| 3,708,115 | + | +0.5 | |||
| 3,708,115 | + | -0.2 | |||
| 3,708,116 | - | -0.2 | |||
| 3,708,118 | + | -0.0 | |||
| 3,708,118 | + | +0.1 | |||
| 3,708,118 | + | +0.1 | |||
| 3,708,135 | + | -0.6 | |||
| 3,708,223 | + | PS417_16975 | 0.16 | -0.2 | |
| 3,708,223 | + | PS417_16975 | 0.16 | +0.3 | |
| 3,708,224 | - | PS417_16975 | 0.16 | -0.2 | |
| 3,708,224 | - | PS417_16975 | 0.16 | -1.1 | |
| 3,708,629 | - | PS417_16975 | 0.79 | -0.8 | |
| 3,708,689 | - | PS417_16975 | 0.88 | -3.1 | |
| 3,708,809 | + | +0.2 | |||
| 3,708,822 | + | -0.4 | |||
| 3,708,823 | - | -0.1 | |||
| 3,708,850 | - | +0.1 | |||
| 3,708,900 | + | +0.5 | |||
| 3,709,076 | - | PS417_16980 | 0.17 | -0.6 | |
| 3,709,076 | - | PS417_16980 | 0.17 | +0.4 | |
| 3,709,101 | + | PS417_16980 | 0.20 | -1.0 | |
| 3,709,102 | - | PS417_16980 | 0.20 | -0.1 | |
| 3,709,102 | - | PS417_16980 | 0.20 | -0.0 | |
| 3,709,102 | - | PS417_16980 | 0.20 | -0.2 | |
| 3,709,209 | - | PS417_16980 | 0.34 | +0.4 | |
| 3,709,209 | - | +2.0 | |||
| 3,709,323 | + | PS417_16980 | 0.48 | +0.7 |
Or see this region's nucleotide sequence