Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10515 and PS417_10520 are separated by 11 nucleotides PS417_10520 and PS417_10525 are separated by 34 nucleotides PS417_10525 and PS417_10530 are separated by 133 nucleotides
PS417_10515: PS417_10515 - acetyl-CoA acetyltransferase, at 2,243,843 to 2,245,024
_10515
PS417_10520: PS417_10520 - succinyl-CoA:3-ketoacid-CoA transferase, at 2,245,036 to 2,245,695
_10520
PS417_10525: PS417_10525 - succinyl-CoA:3-ketoacid-CoA transferase, at 2,245,730 to 2,246,428
_10525
PS417_10530: PS417_10530 - LysR family transcriptional regulator, at 2,246,562 to 2,247,467
_10530
Position (kb)
2245
2246 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2244.174 kb on - strand, within PS417_10515 at 2244.174 kb on - strand, within PS417_10515 at 2244.657 kb on + strand, within PS417_10515 at 2244.657 kb on + strand, within PS417_10515 at 2244.657 kb on + strand, within PS417_10515 at 2244.657 kb on + strand, within PS417_10515 at 2244.858 kb on - strand, within PS417_10515 at 2245.184 kb on - strand, within PS417_10520 at 2245.370 kb on - strand, within PS417_10520 at 2245.370 kb on - strand, within PS417_10520 at 2245.370 kb on - strand, within PS417_10520 at 2245.394 kb on - strand, within PS417_10520 at 2245.708 kb on + strand at 2245.731 kb on + strand at 2245.732 kb on - strand at 2245.794 kb on + strand at 2246.489 kb on - strand at 2246.571 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1 remove 2,244,174 - PS417_10515 0.28 -0.3 2,244,174 - PS417_10515 0.28 +0.7 2,244,657 + PS417_10515 0.69 +0.0 2,244,657 + PS417_10515 0.69 -1.0 2,244,657 + PS417_10515 0.69 +0.2 2,244,657 + PS417_10515 0.69 -0.4 2,244,858 - PS417_10515 0.86 -0.3 2,245,184 - PS417_10520 0.22 -0.7 2,245,370 - PS417_10520 0.51 -0.6 2,245,370 - PS417_10520 0.51 -1.5 2,245,370 - PS417_10520 0.51 -0.3 2,245,394 - PS417_10520 0.54 -0.4 2,245,708 + +0.1 2,245,731 + -1.6 2,245,732 - +0.3 2,245,794 + +3.0 2,246,489 - -0.4 2,246,571 - -0.3
Or see this region's nucleotide sequence