Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25925 and PS417_25930 are separated by 11 nucleotides PS417_25930 and PS417_25935 overlap by 4 nucleotides PS417_25935 and PS417_25940 overlap by 29 nucleotides
PS417_25925: PS417_25925 - pyrroloquinoline quinone biosynthesis protein PqqB, at 5,601,366 to 5,602,277
_25925
PS417_25930: PS417_25930 - pyrroloquinoline quinone biosynthesis protein PqqC, at 5,602,289 to 5,603,041
_25930
PS417_25935: PS417_25935 - pyrroloquinoline quinone biosynthesis protein PqqD, at 5,603,038 to 5,603,313
_25935
PS417_25940: PS417_25940 - pyrroloquinoline quinone biosynthesis protein PqqE, at 5,603,285 to 5,604,436
_25940
Position (kb) 5602
5603
5604 Strain fitness (log2 ratio) -1
0
1
2 at 5601.313 kb on - strand at 5601.313 kb on - strand at 5601.382 kb on + strand at 5601.382 kb on + strand at 5602.166 kb on + strand, within PS417_25925 at 5602.166 kb on + strand, within PS417_25925 at 5602.352 kb on + strand at 5602.352 kb on + strand at 5602.353 kb on - strand at 5602.353 kb on - strand at 5602.353 kb on - strand at 5602.357 kb on + strand at 5602.358 kb on - strand at 5602.385 kb on + strand, within PS417_25930 at 5602.385 kb on + strand, within PS417_25930 at 5602.386 kb on - strand, within PS417_25930 at 5602.386 kb on - strand, within PS417_25930 at 5602.386 kb on - strand, within PS417_25930 at 5602.439 kb on + strand, within PS417_25930 at 5602.440 kb on - strand, within PS417_25930 at 5602.443 kb on - strand, within PS417_25930 at 5602.668 kb on - strand, within PS417_25930 at 5602.808 kb on + strand, within PS417_25930 at 5602.808 kb on + strand, within PS417_25930 at 5602.808 kb on + strand, within PS417_25930 at 5602.809 kb on - strand, within PS417_25930 at 5602.809 kb on - strand, within PS417_25930 at 5602.877 kb on + strand, within PS417_25930 at 5602.877 kb on + strand, within PS417_25930 at 5602.878 kb on - strand, within PS417_25930 at 5602.991 kb on + strand at 5603.024 kb on - strand at 5603.037 kb on + strand at 5603.038 kb on - strand at 5603.361 kb on - strand at 5603.564 kb on + strand, within PS417_25940 at 5603.564 kb on + strand, within PS417_25940 at 5603.564 kb on + strand, within PS417_25940 at 5603.565 kb on - strand, within PS417_25940 at 5603.565 kb on - strand, within PS417_25940 at 5603.565 kb on - strand, within PS417_25940 at 5603.565 kb on - strand, within PS417_25940 at 5603.565 kb on - strand, within PS417_25940 at 5603.566 kb on + strand, within PS417_25940 at 5603.871 kb on - strand, within PS417_25940 at 5604.014 kb on + strand, within PS417_25940 at 5604.014 kb on + strand, within PS417_25940
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 5,601,313 - -0.0 5,601,313 - -1.0 5,601,382 + -0.4 5,601,382 + -0.0 5,602,166 + PS417_25925 0.88 +0.6 5,602,166 + PS417_25925 0.88 -0.8 5,602,352 + -0.3 5,602,352 + +0.1 5,602,353 - -0.3 5,602,353 - +0.0 5,602,353 - +0.5 5,602,357 + +0.0 5,602,358 - +0.4 5,602,385 + PS417_25930 0.13 -0.5 5,602,385 + PS417_25930 0.13 +0.6 5,602,386 - PS417_25930 0.13 -0.4 5,602,386 - PS417_25930 0.13 -0.1 5,602,386 - PS417_25930 0.13 +1.8 5,602,439 + PS417_25930 0.20 -0.2 5,602,440 - PS417_25930 0.20 -0.3 5,602,443 - PS417_25930 0.20 -0.1 5,602,668 - PS417_25930 0.50 +0.2 5,602,808 + PS417_25930 0.69 +0.0 5,602,808 + PS417_25930 0.69 -0.3 5,602,808 + PS417_25930 0.69 +1.0 5,602,809 - PS417_25930 0.69 -0.1 5,602,809 - PS417_25930 0.69 -0.5 5,602,877 + PS417_25930 0.78 +0.1 5,602,877 + PS417_25930 0.78 -0.1 5,602,878 - PS417_25930 0.78 +1.6 5,602,991 + -0.5 5,603,024 - -0.3 5,603,037 + +0.4 5,603,038 - -0.1 5,603,361 - -0.5 5,603,564 + PS417_25940 0.24 -0.4 5,603,564 + PS417_25940 0.24 -0.1 5,603,564 + PS417_25940 0.24 -0.2 5,603,565 - PS417_25940 0.24 +0.1 5,603,565 - PS417_25940 0.24 +0.3 5,603,565 - PS417_25940 0.24 +0.6 5,603,565 - PS417_25940 0.24 +0.3 5,603,565 - PS417_25940 0.24 -0.8 5,603,566 + PS417_25940 0.24 -0.5 5,603,871 - PS417_25940 0.51 -0.1 5,604,014 + PS417_25940 0.63 +0.2 5,604,014 + PS417_25940 0.63 -1.4
Or see this region's nucleotide sequence