Experiment: TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02365 and PS417_02370 are separated by 193 nucleotides PS417_02370 and PS417_02375 overlap by 10 nucleotides PS417_02375 and PS417_02380 are separated by 97 nucleotides
PS417_02365: PS417_02365 - hypothetical protein, at 541,695 to 542,441
_02365
PS417_02370: PS417_02370 - ADP-ribose pyrophosphatase, at 542,635 to 543,252
_02370
PS417_02375: PS417_02375 - hypothetical protein, at 543,243 to 543,692
_02375
PS417_02380: PS417_02380 - serine/threonine protein phosphatase, at 543,790 to 544,605
_02380
Position (kb) 543
544 Strain fitness (log2 ratio) -1
0
1 at 542.363 kb on - strand, within PS417_02365 at 542.363 kb on - strand, within PS417_02365 at 542.463 kb on - strand at 542.516 kb on - strand at 542.635 kb on - strand at 542.707 kb on + strand, within PS417_02370 at 542.708 kb on - strand, within PS417_02370 at 543.220 kb on - strand at 543.251 kb on - strand at 543.312 kb on + strand, within PS417_02375 at 543.312 kb on + strand, within PS417_02375 at 543.313 kb on - strand, within PS417_02375 at 543.435 kb on + strand, within PS417_02375 at 543.511 kb on - strand, within PS417_02375 at 543.511 kb on - strand, within PS417_02375 at 543.573 kb on + strand, within PS417_02375 at 543.573 kb on + strand, within PS417_02375 at 543.574 kb on - strand, within PS417_02375 at 543.690 kb on + strand at 543.715 kb on - strand at 544.575 kb on + strand at 544.603 kb on + strand at 544.606 kb on + strand at 544.606 kb on + strand at 544.606 kb on + strand at 544.607 kb on - strand at 544.607 kb on - strand at 544.607 kb on - strand at 544.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 remove 542,363 - PS417_02365 0.89 -1.5 542,363 - PS417_02365 0.89 +0.7 542,463 - +0.3 542,516 - +0.2 542,635 - -0.3 542,707 + PS417_02370 0.12 -0.6 542,708 - PS417_02370 0.12 -0.3 543,220 - -0.5 543,251 - +0.8 543,312 + PS417_02375 0.15 -0.1 543,312 + PS417_02375 0.15 -0.1 543,313 - PS417_02375 0.16 +0.6 543,435 + PS417_02375 0.43 +0.5 543,511 - PS417_02375 0.60 +0.1 543,511 - PS417_02375 0.60 -0.2 543,573 + PS417_02375 0.73 +0.6 543,573 + PS417_02375 0.73 +0.2 543,574 - PS417_02375 0.74 +0.2 543,690 + -0.1 543,715 - -0.1 544,575 + +0.1 544,603 + +0.3 544,606 + +0.7 544,606 + -1.1 544,606 + +0.8 544,607 - -1.1 544,607 - -0.3 544,607 - +0.1 544,613 - -0.2
Or see this region's nucleotide sequence