Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04405 and PS417_04410 are separated by 25 nucleotides PS417_04410 and PS417_04415 are separated by 8 nucleotides PS417_04415 and PS417_04420 are separated by 128 nucleotides
PS417_04405: PS417_04405 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 979,775 to 981,040
_04405
PS417_04410: PS417_04410 - hypothetical protein, at 981,066 to 981,245
_04410
PS417_04415: PS417_04415 - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 981,254 to 981,889
_04415
PS417_04420: PS417_04420 - histidinol dehydrogenase, at 982,018 to 983,340
_04420
Position (kb)
981
982 Strain fitness (log2 ratio)
-1
0
1 at 981.056 kb on - strand at 981.056 kb on - strand at 981.149 kb on + strand, within PS417_04410 at 981.150 kb on - strand, within PS417_04410 at 981.182 kb on - strand, within PS417_04410 at 981.227 kb on + strand, within PS417_04410 at 981.425 kb on + strand, within PS417_04415 at 981.425 kb on + strand, within PS417_04415 at 981.464 kb on - strand, within PS417_04415 at 981.483 kb on - strand, within PS417_04415 at 981.500 kb on + strand, within PS417_04415 at 981.501 kb on - strand, within PS417_04415 at 981.501 kb on - strand, within PS417_04415 at 981.501 kb on - strand, within PS417_04415 at 981.623 kb on + strand, within PS417_04415 at 981.624 kb on - strand, within PS417_04415 at 981.650 kb on - strand, within PS417_04415 at 981.972 kb on - strand at 982.002 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 981,056 - -0.7 981,056 - -0.1 981,149 + PS417_04410 0.46 +0.0 981,150 - PS417_04410 0.47 -1.3 981,182 - PS417_04410 0.64 -0.7 981,227 + PS417_04410 0.89 -0.7 981,425 + PS417_04415 0.27 +0.1 981,425 + PS417_04415 0.27 +0.5 981,464 - PS417_04415 0.33 -0.1 981,483 - PS417_04415 0.36 -0.4 981,500 + PS417_04415 0.39 -0.5 981,501 - PS417_04415 0.39 -0.1 981,501 - PS417_04415 0.39 -0.3 981,501 - PS417_04415 0.39 -0.7 981,623 + PS417_04415 0.58 -0.3 981,624 - PS417_04415 0.58 -0.2 981,650 - PS417_04415 0.62 +0.2 981,972 - -0.2 982,002 + +0.5
Or see this region's nucleotide sequence