Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_04080 and PS417_04085 are separated by 258 nucleotides PS417_04085 and PS417_04090 are separated by 90 nucleotides
PS417_04080: PS417_04080 - peptide ABC transporter substrate-binding protein, at 916,371 to 917,999
_04080
PS417_04085: PS417_04085 - peptide ABC transporter substrate-binding protein, at 918,258 to 919,859
_04085
PS417_04090: PS417_04090 - hypothetical protein, at 919,950 to 920,663
_04090
Position (kb)
918
919
920 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 917.270 kb on + strand, within PS417_04080 at 917.656 kb on - strand, within PS417_04080 at 917.656 kb on - strand, within PS417_04080 at 917.869 kb on + strand at 917.993 kb on + strand at 917.994 kb on - strand at 918.068 kb on - strand at 918.068 kb on - strand at 918.068 kb on - strand at 918.166 kb on - strand at 918.232 kb on + strand at 918.259 kb on + strand at 918.259 kb on + strand at 918.263 kb on + strand at 918.263 kb on + strand at 918.263 kb on + strand at 918.264 kb on - strand at 918.264 kb on - strand at 918.264 kb on - strand at 918.268 kb on - strand at 918.371 kb on + strand at 918.372 kb on - strand at 918.523 kb on + strand, within PS417_04085 at 918.523 kb on + strand, within PS417_04085 at 918.575 kb on - strand, within PS417_04085 at 918.575 kb on - strand, within PS417_04085 at 918.617 kb on - strand, within PS417_04085 at 918.631 kb on + strand, within PS417_04085 at 918.631 kb on + strand, within PS417_04085 at 918.632 kb on - strand, within PS417_04085 at 918.778 kb on - strand, within PS417_04085 at 918.805 kb on + strand at 918.805 kb on + strand, within PS417_04085 at 918.832 kb on + strand, within PS417_04085 at 918.833 kb on - strand, within PS417_04085 at 918.833 kb on - strand, within PS417_04085 at 918.976 kb on + strand, within PS417_04085 at 918.977 kb on - strand, within PS417_04085 at 918.977 kb on - strand, within PS417_04085 at 918.977 kb on - strand, within PS417_04085 at 919.050 kb on - strand, within PS417_04085 at 919.174 kb on + strand, within PS417_04085 at 919.193 kb on - strand, within PS417_04085 at 919.301 kb on - strand, within PS417_04085 at 919.445 kb on - strand, within PS417_04085 at 919.462 kb on + strand, within PS417_04085 at 919.597 kb on + strand, within PS417_04085 at 919.772 kb on - strand at 919.772 kb on - strand at 919.883 kb on + strand at 919.883 kb on + strand at 919.883 kb on + strand at 919.883 kb on + strand at 919.883 kb on + strand at 919.883 kb on + strand at 919.884 kb on - strand at 919.884 kb on - strand at 919.884 kb on - strand at 919.884 kb on - strand at 919.884 kb on - strand at 919.884 kb on - strand at 919.887 kb on + strand at 919.887 kb on + strand at 919.887 kb on + strand at 919.887 kb on + strand at 919.887 kb on + strand at 919.888 kb on - strand at 919.888 kb on - strand at 919.888 kb on - strand at 919.888 kb on - strand at 919.888 kb on - strand at 919.888 kb on - strand at 920.075 kb on - strand, within PS417_04090 at 920.122 kb on + strand, within PS417_04090 at 920.122 kb on + strand, within PS417_04090 at 920.213 kb on + strand, within PS417_04090 at 920.572 kb on + strand, within PS417_04090 at 920.572 kb on + strand, within PS417_04090 at 920.573 kb on - strand, within PS417_04090 at 920.679 kb on + strand at 920.700 kb on + strand at 920.701 kb on - strand at 920.826 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 917,270 + PS417_04080 0.55 +0.3 917,656 - PS417_04080 0.79 +0.5 917,656 - PS417_04080 0.79 +0.4 917,869 + -1.4 917,993 + +0.1 917,994 - +0.1 918,068 - +0.2 918,068 - -0.4 918,068 - -0.2 918,166 - +0.2 918,232 + -0.2 918,259 + +0.3 918,259 + -1.0 918,263 + -1.7 918,263 + +0.4 918,263 + -0.2 918,264 - +0.4 918,264 - -0.8 918,264 - +0.3 918,268 - -1.3 918,371 + -0.1 918,372 - +1.1 918,523 + PS417_04085 0.17 -0.2 918,523 + PS417_04085 0.17 -0.7 918,575 - PS417_04085 0.20 -0.3 918,575 - PS417_04085 0.20 +0.1 918,617 - PS417_04085 0.22 +0.2 918,631 + PS417_04085 0.23 -2.1 918,631 + PS417_04085 0.23 -0.4 918,632 - PS417_04085 0.23 -0.1 918,778 - PS417_04085 0.32 +0.2 918,805 + +0.9 918,805 + PS417_04085 0.34 -0.3 918,832 + PS417_04085 0.36 -0.2 918,833 - PS417_04085 0.36 +0.3 918,833 - PS417_04085 0.36 -0.5 918,976 + PS417_04085 0.45 +0.1 918,977 - PS417_04085 0.45 +0.7 918,977 - PS417_04085 0.45 -0.0 918,977 - PS417_04085 0.45 +0.0 919,050 - PS417_04085 0.49 +0.3 919,174 + PS417_04085 0.57 +0.0 919,193 - PS417_04085 0.58 +0.2 919,301 - PS417_04085 0.65 +0.7 919,445 - PS417_04085 0.74 +0.3 919,462 + PS417_04085 0.75 -0.1 919,597 + PS417_04085 0.84 -0.1 919,772 - +0.2 919,772 - -1.7 919,883 + -0.7 919,883 + +0.0 919,883 + -0.7 919,883 + -0.0 919,883 + -1.8 919,883 + -0.4 919,884 - +0.1 919,884 - +0.7 919,884 - -1.8 919,884 - -0.3 919,884 - +0.2 919,884 - +0.7 919,887 + -1.4 919,887 + +0.0 919,887 + +0.6 919,887 + +0.7 919,887 + -0.2 919,888 - -0.1 919,888 - +2.2 919,888 - -0.1 919,888 - +0.6 919,888 - -0.4 919,888 - +0.7 920,075 - PS417_04090 0.18 -0.2 920,122 + PS417_04090 0.24 +0.7 920,122 + PS417_04090 0.24 -0.7 920,213 + PS417_04090 0.37 -0.2 920,572 + PS417_04090 0.87 +0.0 920,572 + PS417_04090 0.87 -1.3 920,573 - PS417_04090 0.87 +0.5 920,679 + -0.0 920,700 + -0.0 920,701 - +0.1 920,826 + -0.2
Or see this region's nucleotide sequence