Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00375 and PS417_00380 overlap by 8 nucleotides PS417_00380 and PS417_00385 overlap by 1 nucleotides PS417_00385 and PS417_00390 are separated by 67 nucleotides
PS417_00375: PS417_00375 - membrane protein, at 73,464 to 74,252
_00375
PS417_00380: PS417_00380 - zinc ABC transporter ATP-binding protein, at 74,245 to 75,030
_00380
PS417_00385: PS417_00385 - Fur family transcriptional regulator, at 75,030 to 75,512
_00385
PS417_00390: PS417_00390 - ABC transporter substrate-binding protein, at 75,580 to 76,500
_00390
Position (kb) 75
76 Strain fitness (log2 ratio) -2
-1
0
1
2 at 74.155 kb on + strand, within PS417_00375 at 74.262 kb on + strand at 74.348 kb on + strand, within PS417_00380 at 74.550 kb on - strand at 74.550 kb on - strand, within PS417_00380 at 75.347 kb on - strand, within PS417_00385 at 75.543 kb on - strand at 75.545 kb on + strand at 75.546 kb on - strand at 75.660 kb on - strand at 75.756 kb on + strand, within PS417_00390 at 75.756 kb on + strand, within PS417_00390 at 75.757 kb on - strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.760 kb on + strand, within PS417_00390 at 75.761 kb on - strand, within PS417_00390 at 76.099 kb on - strand, within PS417_00390 at 76.174 kb on + strand, within PS417_00390 at 76.259 kb on + strand, within PS417_00390
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 74,155 + PS417_00375 0.88 -0.1 74,262 + -0.3 74,348 + PS417_00380 0.13 -0.0 74,550 - +2.2 74,550 - PS417_00380 0.39 -0.2 75,347 - PS417_00385 0.66 -0.6 75,543 - +0.1 75,545 + -1.3 75,546 - -0.4 75,660 - -1.3 75,756 + PS417_00390 0.19 -2.6 75,756 + PS417_00390 0.19 -0.3 75,757 - PS417_00390 0.19 -0.5 75,760 + PS417_00390 0.20 -0.3 75,760 + PS417_00390 0.20 +0.8 75,761 - PS417_00390 0.20 +0.0 76,099 - PS417_00390 0.56 -0.3 76,174 + PS417_00390 0.64 -0.2 76,259 + PS417_00390 0.74 -0.2
Or see this region's nucleotide sequence