Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_26170 and PS417_26175 are separated by 10 nucleotides PS417_26175 and PS417_26180 are separated by 123 nucleotides PS417_26180 and PS417_26185 overlap by 4 nucleotides PS417_26185 and PS417_26190 are separated by 15 nucleotides
PS417_26170: PS417_26170 - formyltetrahydrofolate deformylase, at 5,654,431 to 5,655,288
_26170
PS417_26175: PS417_26175 - sarcosine oxidase subunit gamma, at 5,655,299 to 5,655,931
_26175
PS417_26180: PS417_26180 - sarcosine oxidase subunit alpha, at 5,656,055 to 5,659,072
_26180
PS417_26185: PS417_26185 - sarcosine oxidase subunit delta, at 5,659,069 to 5,659,371
_26185
PS417_26190: PS417_26190 - sarcosine oxidase subunit beta, at 5,659,387 to 5,660,637
_26190
Position (kb)
5656
5657
5658
5659
5660 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5655.184 kb on - strand, within PS417_26170 at 5655.188 kb on + strand, within PS417_26170 at 5655.188 kb on + strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.189 kb on - strand, within PS417_26170 at 5655.293 kb on + strand at 5655.486 kb on + strand, within PS417_26175 at 5655.486 kb on + strand, within PS417_26175 at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.914 kb on + strand at 5655.915 kb on - strand at 5655.915 kb on - strand at 5655.915 kb on - strand at 5655.932 kb on - strand at 5656.342 kb on + strand at 5656.409 kb on - strand, within PS417_26180 at 5656.502 kb on + strand, within PS417_26180 at 5656.503 kb on - strand, within PS417_26180 at 5656.571 kb on + strand, within PS417_26180 at 5656.585 kb on - strand, within PS417_26180 at 5656.640 kb on + strand, within PS417_26180 at 5656.671 kb on - strand, within PS417_26180 at 5656.797 kb on + strand, within PS417_26180 at 5656.798 kb on - strand, within PS417_26180 at 5656.830 kb on + strand at 5657.002 kb on - strand, within PS417_26180 at 5657.002 kb on - strand, within PS417_26180 at 5657.148 kb on + strand, within PS417_26180 at 5657.149 kb on - strand, within PS417_26180 at 5657.544 kb on + strand, within PS417_26180 at 5657.544 kb on + strand, within PS417_26180 at 5658.126 kb on + strand, within PS417_26180 at 5658.127 kb on - strand, within PS417_26180 at 5658.583 kb on - strand, within PS417_26180 at 5658.639 kb on + strand, within PS417_26180 at 5658.640 kb on - strand, within PS417_26180 at 5658.690 kb on + strand, within PS417_26180 at 5658.690 kb on + strand, within PS417_26180 at 5658.795 kb on + strand at 5658.796 kb on - strand at 5659.025 kb on - strand at 5659.124 kb on + strand, within PS417_26185 at 5659.124 kb on + strand, within PS417_26185 at 5659.143 kb on - strand, within PS417_26185 at 5659.172 kb on + strand, within PS417_26185 at 5659.172 kb on + strand, within PS417_26185 at 5659.172 kb on + strand, within PS417_26185 at 5659.244 kb on + strand, within PS417_26185 at 5659.245 kb on - strand, within PS417_26185 at 5659.245 kb on - strand, within PS417_26185 at 5659.362 kb on + strand at 5659.362 kb on + strand at 5659.363 kb on - strand at 5659.371 kb on + strand at 5659.737 kb on - strand, within PS417_26190 at 5659.790 kb on + strand, within PS417_26190 at 5659.791 kb on - strand, within PS417_26190
Per-strain Table
Position Strand Gene LocusTag Fraction TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 remove 5,655,184 - PS417_26170 0.88 +0.1 5,655,188 + PS417_26170 0.88 -0.5 5,655,188 + PS417_26170 0.88 -0.0 5,655,189 - PS417_26170 0.88 -0.6 5,655,189 - PS417_26170 0.88 -0.2 5,655,189 - PS417_26170 0.88 -0.2 5,655,293 + -1.9 5,655,486 + PS417_26175 0.30 +0.2 5,655,486 + PS417_26175 0.30 -0.6 5,655,914 + -0.3 5,655,914 + -0.6 5,655,914 + -0.3 5,655,914 + +0.5 5,655,914 + -0.1 5,655,915 - -0.1 5,655,915 - -0.3 5,655,915 - +0.2 5,655,932 - -0.1 5,656,342 + +0.3 5,656,409 - PS417_26180 0.12 -0.2 5,656,502 + PS417_26180 0.15 +0.0 5,656,503 - PS417_26180 0.15 -0.5 5,656,571 + PS417_26180 0.17 +0.1 5,656,585 - PS417_26180 0.18 -0.6 5,656,640 + PS417_26180 0.19 -0.1 5,656,671 - PS417_26180 0.20 +0.7 5,656,797 + PS417_26180 0.25 -0.2 5,656,798 - PS417_26180 0.25 -0.8 5,656,830 + +1.5 5,657,002 - PS417_26180 0.31 +0.1 5,657,002 - PS417_26180 0.31 -0.1 5,657,148 + PS417_26180 0.36 +0.6 5,657,149 - PS417_26180 0.36 -0.1 5,657,544 + PS417_26180 0.49 +1.0 5,657,544 + PS417_26180 0.49 -0.4 5,658,126 + PS417_26180 0.69 +0.2 5,658,127 - PS417_26180 0.69 +0.2 5,658,583 - PS417_26180 0.84 -0.0 5,658,639 + PS417_26180 0.86 +3.3 5,658,640 - PS417_26180 0.86 +0.2 5,658,690 + PS417_26180 0.87 -0.1 5,658,690 + PS417_26180 0.87 -1.7 5,658,795 + -0.4 5,658,796 - -0.3 5,659,025 - +0.2 5,659,124 + PS417_26185 0.18 +0.2 5,659,124 + PS417_26185 0.18 -0.3 5,659,143 - PS417_26185 0.24 +0.1 5,659,172 + PS417_26185 0.34 -1.0 5,659,172 + PS417_26185 0.34 +0.3 5,659,172 + PS417_26185 0.34 +0.2 5,659,244 + PS417_26185 0.58 -0.6 5,659,245 - PS417_26185 0.58 -0.2 5,659,245 - PS417_26185 0.58 +0.6 5,659,362 + -0.7 5,659,362 + +0.5 5,659,363 - +0.3 5,659,371 + +0.0 5,659,737 - PS417_26190 0.28 +0.2 5,659,790 + PS417_26190 0.32 +0.1 5,659,791 - PS417_26190 0.32 +0.1
Or see this region's nucleotide sequence