Experiment: TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt PS417_24770 and PS417_24775 are separated by 177 nucleotides PS417_24775 and PS417_24780 are separated by 105 nucleotides PS417_24780 and PS417_24785 are separated by 29 nucleotides PS417_24785 and PS417_24790 are separated by 138 nucleotides  
        
        PS417_24770: PS417_24770 - murein hydrolase transporter LrgA, at 5,372,714 to 5,373,067 
         
        _24770 
         
        
        PS417_24775: PS417_24775 - dehydratase, at 5,373,245 to 5,373,700 
         
        _24775 
         
        
        PS417_24780: PS417_24780 - peptidase C13, at 5,373,806 to 5,375,524 
         
        _24780 
         
        
        PS417_24785: PS417_24785 - nucleoside-diphosphate sugar epimerase, at 5,375,554 to 5,376,195 
         
        _24785 
         
        
        PS417_24790: PS417_24790 - hypothetical protein, at 5,376,334 to 5,376,618 
         
        _24790 
         Position (kb)  
5373 
 
5374 
 
5375 
 
5376 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.060 kb on - strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.100 kb on + strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.101 kb on - strand at 5373.116 kb on - strand at 5373.175 kb on + strand at 5373.175 kb on + strand at 5373.176 kb on - strand at 5373.176 kb on - strand at 5373.190 kb on + strand at 5373.214 kb on + strand at 5373.214 kb on + strand at 5373.251 kb on + strand at 5373.252 kb on - strand at 5373.252 kb on - strand at 5373.255 kb on + strand at 5373.255 kb on + strand at 5373.255 kb on + strand at 5373.256 kb on - strand at 5373.256 kb on - strand at 5373.331 kb on + strand, within PS417_24775 at 5373.331 kb on + strand, within PS417_24775 at 5373.510 kb on - strand, within PS417_24775 at 5373.510 kb on - strand, within PS417_24775 at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.665 kb on + strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.666 kb on - strand at 5373.689 kb on + strand at 5373.723 kb on + strand at 5373.740 kb on + strand at 5373.740 kb on + strand at 5373.741 kb on - strand at 5373.741 kb on - strand at 5373.741 kb on - strand at 5373.744 kb on + strand at 5373.745 kb on - strand at 5373.745 kb on - strand at 5373.751 kb on - strand at 5373.754 kb on + strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5373.755 kb on - strand at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.385 kb on + strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.386 kb on - strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.523 kb on + strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.524 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780 at 5374.593 kb on - strand, within PS417_24780 at 5374.811 kb on + strand, within PS417_24780 at 5374.811 kb on + strand, within PS417_24780 at 5374.811 kb on + strand, within PS417_24780 at 5374.812 kb on - strand, within PS417_24780 at 5375.030 kb on - strand, within PS417_24780 at 5375.030 kb on - strand, within PS417_24780 at 5375.033 kb on + strand, within PS417_24780 at 5375.033 kb on + strand, within PS417_24780 at 5375.033 kb on + strand, within PS417_24780 at 5375.034 kb on - strand, within PS417_24780 at 5375.034 kb on - strand at 5375.034 kb on - strand, within PS417_24780 at 5375.285 kb on + strand, within PS417_24780 at 5375.285 kb on + strand at 5375.451 kb on + strand at 5375.500 kb on + strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.523 kb on - strand at 5375.537 kb on - strand at 5375.557 kb on + strand at 5375.558 kb on - strand at 5375.558 kb on - strand at 5375.574 kb on + strand at 5375.575 kb on - strand at 5375.926 kb on + strand, within PS417_24785 at 5375.927 kb on - strand at 5375.927 kb on - strand, within PS417_24785 at 5376.082 kb on + strand, within PS417_24785 at 5376.193 kb on + strand at 5376.193 kb on + strand at 5376.194 kb on - strand at 5376.194 kb on - strand at 5376.203 kb on + strand at 5376.204 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.272 kb on - strand at 5376.274 kb on + strand at 5376.274 kb on + strand at 5376.274 kb on + strand at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.521 kb on + strand, within PS417_24790 at 5376.522 kb on - strand, within PS417_24790  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   TSB 0.5x with Dimethyl Sulfoxide 0.5 vol%; timepoint 1             remove   5,373,060   -        -0.4   5,373,060   -        +0.3   5,373,060   -        -0.3   5,373,060   -        +0.2   5,373,100   +        -0.0   5,373,100   +        -1.0   5,373,100   +        -0.0   5,373,100   +        -1.2   5,373,101   -        -0.8   5,373,101   -        -0.2   5,373,101   -        -0.3   5,373,101   -        -0.2   5,373,116   -        +0.4   5,373,175   +        +0.5   5,373,175   +        +0.1   5,373,176   -        -0.3   5,373,176   -        -0.4   5,373,190   +        -0.2   5,373,214   +        -1.6   5,373,214   +        -0.3   5,373,251   +        -0.0   5,373,252   -        +0.6   5,373,252   -        -0.1   5,373,255   +        -0.2   5,373,255   +        -0.4   5,373,255   +        -0.0   5,373,256   -        -0.1   5,373,256   -        +1.5   5,373,331   +    PS417_24775   0.19  -0.6   5,373,331   +    PS417_24775   0.19  -0.5   5,373,510   -    PS417_24775   0.58  -0.1   5,373,510   -    PS417_24775   0.58  -0.3   5,373,665   +        +1.1   5,373,665   +        -0.6   5,373,665   +        -0.5   5,373,665   +        -2.1   5,373,665   +        -0.3   5,373,666   -        -0.3   5,373,666   -        -0.2   5,373,666   -        -0.0   5,373,666   -        +0.6   5,373,689   +        -0.4   5,373,723   +        -0.5   5,373,740   +        -0.5   5,373,740   +        -0.3   5,373,741   -        -0.5   5,373,741   -        -1.1   5,373,741   -        -0.1   5,373,744   +        -0.2   5,373,745   -        -0.4   5,373,745   -        -0.0   5,373,751   -        +0.3   5,373,754   +        +0.1   5,373,755   -        +0.0   5,373,755   -        -0.4   5,373,755   -        +0.3   5,373,755   -        -0.5   5,374,385   +    PS417_24780   0.34  -0.6   5,374,385   +    PS417_24780   0.34  -0.3   5,374,385   +    PS417_24780   0.34  +0.2   5,374,385   +    PS417_24780   0.34  -1.0   5,374,385   +    PS417_24780   0.34  -0.5   5,374,386   -    PS417_24780   0.34  -0.6   5,374,386   -    PS417_24780   0.34  -0.2   5,374,386   -    PS417_24780   0.34  -0.6   5,374,386   -    PS417_24780   0.34  -0.7   5,374,523   +    PS417_24780   0.42  -0.4   5,374,523   +    PS417_24780   0.42  -0.5   5,374,523   +    PS417_24780   0.42  -0.3   5,374,524   -    PS417_24780   0.42  -0.8   5,374,524   -    PS417_24780   0.42  -1.1   5,374,524   -    PS417_24780   0.42  -0.6   5,374,593   -    PS417_24780   0.46  -0.5   5,374,593   -    PS417_24780   0.46  -0.5   5,374,593   -    PS417_24780   0.46  -0.3   5,374,811   +    PS417_24780   0.58  -0.0   5,374,811   +    PS417_24780   0.58  +0.3   5,374,811   +    PS417_24780   0.58  -0.4   5,374,812   -    PS417_24780   0.59  -0.8   5,375,030   -    PS417_24780   0.71  -0.7   5,375,030   -    PS417_24780   0.71  +0.0   5,375,033   +    PS417_24780   0.71  -0.3   5,375,033   +    PS417_24780   0.71  +1.2   5,375,033   +    PS417_24780   0.71  -0.8   5,375,034   -    PS417_24780   0.71  -0.1   5,375,034   -        +1.8   5,375,034   -    PS417_24780   0.71  +0.1   5,375,285   +    PS417_24780   0.86  -0.0   5,375,285   +        +1.6   5,375,451   +        -0.2   5,375,500   +        +0.5   5,375,523   -        -0.2   5,375,523   -        -0.5   5,375,523   -        +0.1   5,375,523   -        -0.6   5,375,537   -        -0.1   5,375,557   +        +0.4   5,375,558   -        +0.4   5,375,558   -        +0.1   5,375,574   +        -0.1   5,375,575   -        -2.3   5,375,926   +    PS417_24785   0.58  -0.6   5,375,927   -        +0.9   5,375,927   -    PS417_24785   0.58  -0.1   5,376,082   +    PS417_24785   0.82  -0.3   5,376,193   +        +0.6   5,376,193   +        +0.1   5,376,194   -        -0.3   5,376,194   -        -0.1   5,376,203   +        -0.1   5,376,204   -        -0.2   5,376,272   -        -0.7   5,376,272   -        -0.8   5,376,272   -        -0.4   5,376,272   -        -0.1   5,376,274   +        -0.3   5,376,274   +        -0.7   5,376,274   +        -0.4   5,376,521   +    PS417_24790   0.66  -0.8   5,376,521   +    PS417_24790   0.66  -0.1   5,376,521   +    PS417_24790   0.66  +0.4   5,376,521   +    PS417_24790   0.66  -0.2   5,376,521   +    PS417_24790   0.66  -0.0   5,376,522   -    PS417_24790   0.66  +0.0 
 
Or see this region's nucleotide sequence